Impact of ZnO nanoparticles on MagA gene expression in Klebsiella pneumoniae isolated from diabetic foot ulcers DOI Open Access

Mustafa Muhammed Jadooa,

Haider Turky Mousa Al-Mousawi,

Nadhim Mushtaq Hashim Al-bderee

et al.

Asia-Pacific Journal of Molecular Biology and Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 248 - 256

Published: Sept. 26, 2024

Diabetes mellitus (DM) is an accumulation of glucose in the blood. Diabetic divided into gestational diabetes, type 1 and 2. Complications diabetes include diabetic foot ulcers. The most important complication ulcers bacterial infection, which leads to gangrene when various types antibiotics fail prevent infections. To explore effect synthetic zinc oxide nanoparticles (ZnONPs) on expression MagA gene Klebsiella pneumoniae isolated from ulcer cases. ZnONPs were biosynthesized by Aspergillus niger characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), UV-visible spectroscopy. inhibitory K. isolates was examined determining minimum concentration (MIC) ZnONPs. Besides, values before after exposure would be RT-qPCR, with 16SrRNA as reference gene. According SEM XRD results, are form nanocrystals have small spherical crystals average size 13.5 nm. highest optical density synthesized obtained at 390 exhibit a strong inhibition zone against corresponding 125 μg/mL. fold ranged 0.042 0.118. research reveals that antibacterial potential affect strains. results present study indicate can indeed used treatment infectious bacteria.

Language: Английский

Effect of Biosynthesized Silver Nanoparticle Size on Antibacterial and Anti-Biofilm Activity against Pathogenic Multi-Drug Resistant Bacteria DOI Creative Commons

Anas Yahya Ali,

Abd-Alrahman Khalid Alani,

Barakat O. Ahmed

et al.

OpenNano, Journal Year: 2024, Volume and Issue: 20, P. 100213 - 100213

Published: Aug. 23, 2024

Language: Английский

Citations

14
Insecticidal Activity of Green Synthesized Silver Nanoparticles from Pelargonium Citronellum against Citrus Mealybug, Planococcus Citri DOI Open Access
Thamer Y. Mutter, Omar M. Hassan

Plant Protection, Journal Year: 2024, Volume and Issue: 8(1), P. 69 - 78

Published: March 27, 2024

Planococcus citri is a common insect pest that poses serious threat to citrus crops. Treatment of with chemical pesticides causes harm humans, animals, and the environment. This study aimed use silver nanoparticles as potential alternatives control this agricultural pest. Silver (AgNPs) have been identified using X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM). An absorbance peak at 425 nm in UV-Vis spectrum showed existence AgNPs due plasmon resonance. XRD patterns verified possess highly crystalline structure. SEM imaging were spherical shape an average size 12.4 nm. Toxicological tests prepared they are effective insecticidal activities different stages mealybug. Using synthesized for more than 72 hours treatment concentration 100 ppm resulted highest killing rates 40.0, 68.0, 70.0% development three mealybug, including eggs, first age nymphs, adult females, respectively. The lowest percentage efficacy was recorded 5 ppm, reaching 62.75 4.6 % after 3 7 days, To our knowledge, it noteworthy report successfully from extract Pelargonium citronellum time. results demonstrated natural extracts source manage mealybugs.

Language: Английский

Citations

9
Construction and Characterization of 5,6-O-Isopropylidene-L-Ascorbic Acid-Loaded Silver and Manganese Dioxide Nanoparticles: Cytotoxicity Study, Antibacterial, and Antioxidant Activities DOI
Mouhaned Y. Al-darwesh,

Layth L. Hamid,

Sattar S. Ibrahim

et al.

Materials Chemistry and Physics, Journal Year: 2025, Volume and Issue: unknown, P. 130517 - 130517

Published: Feb. 1, 2025

Language: Английский

Citations

1
Date Palm Pollen: A Novel Medium for Bacterial Culture and Biosynthesis of Antibacterial Chromium Oxide Nanoparticles DOI Creative Commons

Layth L. Hamid,

Thamer Y. Mutter, Asmiet Ramizy

et al.

OpenNano, Journal Year: 2024, Volume and Issue: 19, P. 100212 - 100212

Published: Aug. 15, 2024

Medical companies and research centers seek cost-effective alternative culture media containing the necessary nutritional requirements for bacterial cultivation because of high manufacturing production costs. Date palm pollen (DPP) is an inexpensive rich natural plant resource that can be easily collected in large quantities. In this study, DPP was used to prepare a novel enriched medium successfully cultivated pathogenic bacteria Streptomyces spp. without any additives at low concentration 10 g per 1 L distilled water (DW). Active compounds were detected by GCMS–, results show highest ratio urea (45 %) among other components. For first time, synthesize Cr2O3 NPs environment-friendly green method. plays important role as reducing stabilizing agent biosynthesis NPs. The obtained characterized using UV–Vis, FTIR, XRD SEM. agar disk diffusion ranging between 12 – 20 mm while MIC 1.56 25 U/mL indicated effectiveness antibacterial activity against wide range bacteria. showed inhibition reach 78 % biofilms produced P. aeruginosa. later studies we expect selective after adding certain materials or antibiotics. anti-biofilm could promising medical pharmaceutical applications.

Language: Английский

Citations

7
Antibacterial, Antibiofilm, and Quorum Quenching Properties of Biogenic Chitosan Silver Nanoparticles Against Staphylococcus aureus DOI

Reem Mahdi Saleh,

Omar M. Hassan

BioNanoScience, Journal Year: 2024, Volume and Issue: 14(4), P. 4456 - 4468

Published: July 31, 2024

Language: Английский

Citations

5
Construction and Characterization of Polyethylene Glycol/Sodium Alginate Hydrogel Loaded With Zirconia Nanoparticles: Potential Antibacterial and Antibiofilm Agent to Inhibit Dental Caries In Vitro and In Vivo DOI Creative Commons

Layth L. Hamid,

Roqaya S. Zakir,

Nur indah noviyanti Rahman

et al.

Microscopy Research and Technique, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Biofilm formation on tooth surfaces is a primary contributor to dental caries and periodontal diseases. Streptococcus mutans recognized for its role in biofilm production, significantly influencing the development of caries. Key virulence factors associated with S. biofilms include acid tolerance, synthesis exopolysaccharides (EPS). This study presents novel approach by focusing loading biosynthesized zirconia nanoparticles (ZrO2 NPs) onto polyethylene glycol/sodium alginate (PEG/SA) hydrogel nanocomposite, evaluating their effects biofilm-forming ability both vivo vitro. ZrO2 NPs were using Citrus aurantifolia (C. aurantifolia) extract incorporated into PEG/SA beads through sol-gel process. The PEG/SA/ZrO2 nanocomposite was confirmed diverse analyzes, including UV-visible spectroscopy, particle size measurement, morphology examination, spectral analysis, thermal gravimetric analysis (TGA) hemolysis studies. average approximately 26 nm, while exhibited highly porous, sheet-like surface structure. In vitro results demonstrated inhibition zones 30 28 mm against mutans, respectively, minimum inhibitory concentration (MIC) 12.5 mg/mL. growth curve indicated complete decline an 87% reduction when treated beads. SEM revealed that cells appeared lysed or crumpled, losing characteristic coccal shape after exposure Additionally, images effective prevention attachment teeth encapsulated hydrogel, altering mature developed treatment. Finally, incorporation hydrogels demonstrates significant potential as strategy inhibiting may serve promising topical agent reducing Further studies could explore long-term efficacy clinical applications this oral health care.

Language: Английский

Citations

0
A comparative study on the efficacy of nano-formulated Melia azedarach (L.) ethanolic extract and cyantraniliprole in controlling greenhouse whitefly ( Trialeurodes vaporariorum Westwood) populations DOI
Kimia Zeinali, Kamal Ahmadi

Archives of Phytopathology and Plant Protection, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 17

Published: April 1, 2025

Language: Английский

Citations

0
Silver Nanoparticles Synthesized from Acacia glauca Leaves: A Promising Agent Targeting Virulent Genes of Staphylococcus aureus DOI Creative Commons

Reem Mahdi Saleh,

Omar M. Hassan

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 25, 2024

Abstract Nowadays, antibiotics are the only therapeutic agents against Staphylococcus aureus infection. Widespread antibiotic resistance poses a threat to world. Several studies have attempted test whether alternative acting alone or in synergy with can overcome this problem. For many years, silver nanoparticles demonstrated multi-level action targeting physiological activities of bacteria. The study aimed synthesize by green method from Acacia glauca leaves stabilized chitosan (Ch-AgNPs) and evaluate their effect expression set quorum sensing virulent genes MDR S. aureus. Ch-AgNPs were characterized physicochemical tests including UV, X-ray, FTIR, TEM, FESEM, XRD, zeta potential. Minimum inhibition concentration (MIC) was used detect antibacterial activity Ch-AgNPs. After MIC treatment, growth curve aureus plotted. (gyrb, AgrA, AgrB, RNAIII, mecA, rot, spa, hla, coa, psm) evaluated before after exposure quantitative real-time PCR. dark brown color primary indicator formation. showed absorption at 430 nm. particles had round regular shape an average size 8 synthesized high degree crystallinity, thin peaks 2θ° 38.200 44.250. At +33 mV, potential confirmed colloidal stability. 2.1 μg/mL, inhibiting All identified significant decrease RT-PCR subMIC Ch-AgNPs, except for gyrb gene, which is housekeeping gene. Silver made Acacia glauca be effective agent. They exert effects molecular level virulence

Language: Английский

Citations

0
Impact of ZnO nanoparticles on MagA gene expression in Klebsiella pneumoniae isolated from diabetic foot ulcers DOI Open Access

Mustafa Muhammed Jadooa,

Haider Turky Mousa Al-Mousawi,

Nadhim Mushtaq Hashim Al-bderee

et al.

Asia-Pacific Journal of Molecular Biology and Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 248 - 256

Published: Sept. 26, 2024

Diabetes mellitus (DM) is an accumulation of glucose in the blood. Diabetic divided into gestational diabetes, type 1 and 2. Complications diabetes include diabetic foot ulcers. The most important complication ulcers bacterial infection, which leads to gangrene when various types antibiotics fail prevent infections. To explore effect synthetic zinc oxide nanoparticles (ZnONPs) on expression MagA gene Klebsiella pneumoniae isolated from ulcer cases. ZnONPs were biosynthesized by Aspergillus niger characterized using scanning electron microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), UV-visible spectroscopy. inhibitory K. isolates was examined determining minimum concentration (MIC) ZnONPs. Besides, values before after exposure would be RT-qPCR, with 16SrRNA as reference gene. According SEM XRD results, are form nanocrystals have small spherical crystals average size 13.5 nm. highest optical density synthesized obtained at 390 exhibit a strong inhibition zone against corresponding 125 μg/mL. fold ranged 0.042 0.118. research reveals that antibacterial potential affect strains. results present study indicate can indeed used treatment infectious bacteria.

Language: Английский

Citations

0