Ameliorative effect of rhizobacteria Bradyrhizobium japonicum on antioxidant enzymes, cell viability and biochemistry in tomato plant under nematode stress
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: March 7, 2025
Root
Knot
Nematode
(Meloidogyne
incognita)
is
a
major
agricultural
pest
that
significantly
reduces
crop
yield.
This
study
investigates
the
nematicidal
potential
of
Bradyrhizobium
japonicum
strain
11477
against
M.
incognita
to
regulate
its
pathogenicity
in
Solanum
lycopersicum.
Tomato
seeds
were
treated
with
bacterial
cells
and
supernatant,
grown
under
controlled
conditions
later
infested
nematode
juveniles
(5J2/seedling).
After
10
days,
infestation
led
reduced
seedling
growth,
lower
root
shoot
biomass
decreased
photosynthetic
pigments.
It
also
triggered
oxidative
stress,
as
indicated
by
elevated
stress
markers.
Enzymatic
non-enzymatic
antioxidants
along
phenolic
compounds
showed
increased
activity
response
nematode-induced
stress.
However,
B.
treatment
gall
formation,
improved
plant
growth
enhanced
biochemical
histochemical
attributes.
Rhizobacteria
alleviated
indices,
strengthened
antioxidant
defenses
metabolite
production.
Confocal
microscopy
revealed
hydrogen
peroxide
localization,
glutathione
content
nuclear
membrane
damage
apices,
correlating
defense
responses.
highlights
potent
biocontrol
agent
enhances
resilience
incognita.
Notably,
this
first
report
on
impact
leguminous
rhizobacterium
non-leguminous
tomato
plant,
providing
new
insights
into
for
sustainable
management.
Language: Английский
Population Dynamics and Virulence Patterns of Root‐Knot Nematodes (Meloidogyne spp.) on Tomato in Poonch Highlands, Azad Jammu and Kashmir, Pakistan
Journal of Phytopathology,
Journal Year:
2025,
Volume and Issue:
173(2)
Published: March 1, 2025
ABSTRACT
Root‐knot
nematodes
(RKNs)
are
root
parasites
of
tomatoes.
The
population
dynamics
RKNs,
including
their
incidence
and
prevalence,
need
to
be
explored
in
tomato
crops
from
the
Poonch
Highlands
Azad
Jammu
Kashmir.
Overall,
50.6%
surveyed
crop
was
found
infested,
with
disease
severity
(galling
index)
ranging
1
9.
highest
RKN
prevalence
(77.8%)
recorded
Haveli,
followed
by
(64.2%),
Sudhnuti
(47.6%)
lowest
Bagh
(41.6%).
index
2–9)
observed
district,
district
(1–9),
while
it
Haveli
(4–6),
(2–7).
Morphological
molecular
diagnostics
confirmed
presence
tropical
species
affecting
tomato.
Three
major
were
identified.
Meloidogyne
javanica
most
prevalent,
occurring
at
27.6%
sites,
M
eloidogyne
incognita
(23.5%)
arenaria
(18.8%).
Mixed
populations
fewer
than
5%
sites
for
each
combination.
M.
(66.7%)
(9.5%)
Sudhnuti.
exhibited
similar
distribution
patterns
across
all
districts,
20.2%
33.3%
17.0%
44.4%,
respectively.
Mixed‐population
infestations
relatively
low
compared
Haveli.
Ecological
diversity
influence
virulence
patterns.
This
study
highlights
species,
likely
representing
an
indigenous
fauna
impacting
under
field
conditions
temperate
highlands.
Language: Английский