Streptomyces: The biofactory of secondary metabolites

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 29, 2022

Natural products derived from microorganisms serve as a vital resource of valuable pharmaceuticals and therapeutic agents. Streptomyces is the most ubiquitous bacterial genus in environments with prolific capability to produce diverse natural significant biological activities medicine, environments, food industries, agronomy sectors. However, many remain unexplored among . It exigent develop novel antibiotics, agrochemicals, anticancer medicines, etc., due fast growth resistance cancer chemotherapeutics, pesticides. This review article focused secreted by their function importance curing diseases agriculture. Moreover, it discussed genomic-driven drug discovery strategies also gave future perspective for development

Language: Английский

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Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 21, 2024

Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a major class of natural products with diverse chemical structures potent biological activities. A vast majority RiPP gene clusters remain unexplored in microbial genomes, which is partially due to the lack rapid efficient heterologous expression systems for characterization biosynthesis. Here, we report unified biocatalysis (UniBioCat) system based on cell-free biosynthesis engineering RiPPs. We demonstrate UniBioCat by reconstituting full biosynthetic pathway de novo salivaricin B, lanthipeptide RiPP. Next, delete several protease/peptidase genes from source strain enhance performance UniBioCat, then can synthesize screen B variants enhanced antimicrobial activity. Finally, show that generalizable synthesizing evaluating bioactivity ten uncharacterized lanthipeptides. expect accelerate discovery, characterization, synthesis

Language: Английский

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A review of current antibiotic resistance and promising antibiotics with novel modes of action to combat antibiotic resistance

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(11)

Published: Oct. 20, 2023

Language: Английский

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Ribosomally synthesized peptides, foreground players in microbial interactions: recent developments and unanswered questions

Natural Product Reports, Journal Year: 2021, Volume and Issue: 39(2), P. 273 - 310

Published: Nov. 10, 2021

It is currently well established that multicellular organisms live in tight association with complex communities of microorganisms including a large number bacteria. These are immersed interaction networks reflecting the relationships between them and host organisms; yet, little known about molecules mechanisms involved these mutual interactions. Ribosomally synthesized peptides, among which bacterial antimicrobial peptides called bacteriocins microcins have been identified as contributing to host-microbe interplays, either unmodified or post-translationally modified peptides. This review will unveil current knowledge on ribosomal peptide-based natural products, their interplay immune system, roles microbial interactions symbioses. include major structural characteristics post-translational modifications, main rules maturation pathways, principal ecological functions they ensure (communication, signalization, competition), especially symbiosis, taking select examples various organisms. Finally, we address unanswered questions provide framework for deciphering big issues inspiring future directions field.

Language: Английский

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The Rare Actinobacterium Crossiella sp. Is a Potential Source of New Bioactive Compounds with Activity against Bacteria and Fungi

Microorganisms, Journal Year: 2022, Volume and Issue: 10(8), P. 1575 - 1575

Published: Aug. 5, 2022

Antimicrobial resistance has become a global problem in recent decades. A gradual reduction drug discoveries led to the current antimicrobial crisis. Caves and other subsurface environments are underexplored thus far, they represent indispensable ecological niches that could offer new molecules of interest medicine biotechnology. We explored Spanish show caves test bioactivity bacteria dwelling walls ceilings, as well airborne bacteria. reported isolation two strains genus Crossiella, likely representing species, isolated from Altamira Cave, Spain. In vitro silico analyses showed inhibition pathogenic Gram-positive Gram-negative bacteria, fungi, taxonomical distance both their closest relative, Crossiella cryophila. The presence an exclusive combination gene clusters involved synthesis lanthipeptides, lasso peptides, nonribosomal peptides polyketides indicates species this source compounds. Overall, there is promising evidence for discovery subterranean environments, which increases possibility identifying bioactive molecules.

Language: Английский

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Discovery of phosphorylated lantibiotics with proimmune activity that regulate the oral microbiome

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(22)

Published: May 22, 2023

Lantibiotics are ribosomally synthesized and posttranslationally modified peptides (RiPPs) that produced by bacteria. Interest in this group of natural products is increasing rapidly as alternatives to conventional antibiotics. Some human microbiome–derived commensals produce lantibiotics impair pathogens’ colonization promote healthy microbiomes. Streptococcus salivarius one the first commensal microbes colonize oral cavity gastrointestinal tract, its biosynthesis RiPPs, called salivaricins, has been shown inhibit growth pathogens. Herein, we report on a phosphorylated class three related collectively referred salivaricin 10, exhibit proimmune activity targeted antimicrobial properties against known pathogens multispecies biofilms. Strikingly, immunomodulatory activities observed include upregulation neutrophil-mediated phagocytosis, promotion antiinflammatory M2 macrophage polarization, stimulation neutrophil chemotaxis—these have attributed phosphorylation site identified N-terminal region peptides. Salivaricin 10 were determined be S. strains found subjects, their dual bactericidal/antibiofilm immunoregulatory may provide new means effectively target infectious while maintaining important microbiota.

Language: Английский

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Discovery and engineering of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products

RSC Chemical Biology, Journal Year: 2023, Volume and Issue: 5(2), P. 90 - 108

Published: Nov. 21, 2023

The major approaches ( i.e. bioactivity-guided screening, genome mining, and biosynthetic engineering) employed in the exploration of RiPP (ribosomally synthesized post-translationally modified peptides) natural products are discussed this review.

Language: Английский

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Enabling Access to Novel Bacterial Biosynthetic Potential From ONT Draft Genomic Data

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(3)

Published: March 1, 2025

Natural products comprise a wide diversity of compounds with range biological activities, including antibiotics, anti-inflammatory and anti-tumoral molecules. However, we can only access small portion these due to various technical difficulties. We have herein developed novel efficient approach for accessing biosynthetic gene clusters (BGCs) that encode natural from soil bacteria. The pipeline uses combination long-read sequencing, antiSMASH BGC identification Transformation-associated recombination (TAR) cloning the BGCs. hypothesized genome assembly using Oxford Nanopore Technology (ONT) sequencing could facilitate detection large BGCs at relatively fast low-cost DNA sequencing. Despite relative low accuracy sequence mistakes high GC content repetitions frequently found in containing bacteria, demonstrate ONT are effective identifying enabling TAR isolate desired vector. applied this on previously non-sequenced myxobacteria Aetherobacter fasciculatus SBSr002. Our enabled us clone unknown into engineering-ready vector, illustrating capabilities powerful cost-effective strategy.

Language: Английский

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Genome mining reveals the biosynthetic potential of a novel Lysinibacillus zambalensis sp. nov., isolated from a hyperalkaline spring

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(5)

Published: April 2, 2025

Language: Английский

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Heterologous Production and Structure Determination of a New Lanthipeptide Sinosporapeptin Using a Cryptic Gene Cluster in an Actinobacterium Sinosporangium siamense

The Journal of Microbiology, Journal Year: 2023, Volume and Issue: 61(6), P. 641 - 648

Published: June 1, 2023

Language: Английский

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