Comparative Assessment of sEV Biogenesis Regulator Proteins in MSCs and HeLa Cells for Elucidating Novel Therapeutic Strategies DOI Creative Commons
Yashvi Sharma, Sujata Mohanty

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract In the intricate landscape of cellular communication, small extracellular vesicles (sEV) originating from endosomes play crucial roles as mediators and have garnered significant attention in theranostics. Our understanding sEV biogenesis largely stems studies on cancer cells, which are vital for diagnostics. However, therapeutics, where mesenchymal stem cell (MSC)-derived emerging investigational new drugs, their pathways remain unexplored. This article explores parallel narratives cells specifically using HeLa MSCs model lines. study investigated key proteins—HRS, STAM, TSG101, ALIX—as identified context MSC-sEV biogenesis. While these proteins show similarities across types, a discernible difference arises primary functions regulating The critical role ALIX MSC biogenesis, particular, underscores its potential target modulating yield regenerative therapies. Through this comparative analysis, we shared molecular signatures, offering insights to guide therapeutic interventions unlock cells.

Language: Английский

Plant-Derived Exosome-like Nanoparticles for Biomedical Applications and Regenerative Therapy DOI Creative Commons
Andari Sarasati, Muhammad Hidayat Syahruddin, Archadian Nuryanti

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(4), P. 1053 - 1053

Published: March 29, 2023

Plant-derived exosome-like nanoparticles (PDENs) comprise various bioactive biomolecules. As an alternative cell-free therapeutic approach, they have the potential to deliver nano-bioactive compounds human body, and thus lead anti-inflammatory, antioxidant, anti-tumor benefits. Moreover, it is known that Indonesia one of herbal centers world, with abundance unexplored sources PDENs. This encouraged further research in biomedical science develop natural richness plants as a source for welfare. study aims verify PDENs purposes, especially regenerative therapy applications, by collecting analyzing data from latest relevant developments.

Language: Английский

Citations

44
The role of exosomes in diagnosis, pathophysiology, and management of Alzheimer's Disease DOI Creative Commons

Rajni Daksh,

Meby Susan Mathew,

Aan Mery Bosco

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: 754, P. 151526 - 151526

Published: Feb. 22, 2025

Language: Английский

Citations

2
Extracellular vesicles and exosome-like nanovesicles as pioneering oral drug delivery systems DOI Creative Commons
Jagannath Mondal, Shameer Pillarisetti, Vijayabhaskarreddy Junnuthula

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 8, 2024

As extracellular vesicle (EV)-based nanotechnology has developed rapidly, it made unprecedented opportunities for nanomedicine possible. EVs and exosome-like nanovesicles (ELNVs) are natural nanocarriers with unique structural, compositional, morphological characteristics that provide excellent physical, chemical, biochemical properties. In this literature, we examine the of EVs, including how they administered orally their therapeutic activity. According to current examples ELNVs oral delivery, milk plant can exert effects through protein, nucleic acid, lipid components. Furthermore, several methods loading drugs into exosomes targeting have been employed investigate capability. Moreover, discuss as potential drug carriers role disease prevention treatment or in future. conclusion, issues associated development from sources such plants, well concerns standardized applications these discussed.

Language: Английский

Citations

14
Unseen Weapons: Bacterial Extracellular Vesicles and the Spread of Antibiotic Resistance in Aquatic Environments DOI Open Access
Muttiah Barathan, Sook Luan Ng, Yogeswaran Lokanathan

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3080 - 3080

Published: March 7, 2024

This paper sheds light on the alarming issue of antibiotic resistance (ABR) in aquatic environments, exploring its detrimental effects ecosystems and public health. It examines multifaceted role use aquaculture, agricultural runoff, industrial waste fostering development dissemination resistant bacteria. The intricate interplay between various environmental factors, horizontal gene transfer, bacterial extracellular vesicles (BEVs) accelerating spread ABR is comprehensively discussed. Various BEVs carrying genes like blaCTX-M, tetA, floR, sul/I, as well their contribution to dominance multidrug-resistant bacteria, are highlighted. potential both a threat tool combating explored, with promising strategies targeted antimicrobial delivery systems probiotic-derived EVs holding significant promise. underscores urgency understanding environments. By unraveling these unseen weapons, we pave way for developing effective mitigate ABR, advocating multidisciplinary approach that includes stringent regulations, enhanced wastewater treatment, adoption sustainable practices aquaculture.

Language: Английский

Citations

14
Brain-Homing Peptide Expression on the Membrane Enhances the Delivery of Exosomes to Neural Cells and Tissue DOI Creative Commons

Jonhoi Smith,

Melvin Field, Kimio Sugaya

et al.

Neuroglia, Journal Year: 2025, Volume and Issue: 6(1), P. 3 - 3

Published: Jan. 4, 2025

Background/Objectives: Glioblastoma (GBM), a highly aggressive grade IV astrocytoma, poses major therapeutic challenge due to the resistance of cancer stem cells (CSCs) existing within its cell population conventional therapies. Recently, we reported that RNA interference targeting CSC protection mechanism significantly improved efficacy. However, challenges remain, including limited transfection efficiency in neural and difficulty crossing blood–brain barrier (BBB). Methods: In this study, investigated potential exosome-mediated delivery cargo GBM by engineering exosomes carry green fluorescent protein (GFP) expressing brain-homing peptide (BHP) on their surface, which has high affinity cells. Results: We found BHP-modified doubled GFP efficacy from 20% 40%, outperforming traditional methods like lipofection vitro. vivo, demonstrated an ability cross BBB targeted brain regions following intranasal subcutaneous administration. Conclusions: These results underscore engineered for efficient enhance against tumors suggest novel avenues delivering biomolecules treatment neurological disorders.

Language: Английский

Citations

1
Momordica charantia-Derived Extracellular Vesicles Provide Antioxidant Protection in Ulcerative Colitis DOI Creative Commons
Feng Wang, Meng Yuan,

Chenqi Shao

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(17), P. 6182 - 6182

Published: Aug. 22, 2023

Plant-derived extracellular vesicles are functional nanovesicles that have significant applications in both disease prevention and treatment, as well for use drug carriers. Momordica charantia is a widely consumed food has medicinal nutritional properties shown intervention diabetes inflammation caused by oxidative damage. In this study, charantia-derived (MCEVs) were extracted demonstrated to excellent antioxidant activity characterization, lipid composition analysis, protein domain vitro measurement. addition, vivo studies indicated the MCEVs could restore ulcerative colitis regulating oxidation inflammatory factors. Therefore, of may be important protecting colon from inflammation, which provides new insights into application drugs or vectors colitis.

Language: Английский

Citations

21
Exosomes: Diagnostic and Therapeutic Implications in Cancer DOI Creative Commons

Hyein Jo,

Kyeonghee Shim,

Dooil Jeoung

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(5), P. 1465 - 1465

Published: May 11, 2023

Exosomes are a subset of extracellular vesicles produced by all cells, and they present in various body fluids. play crucial roles tumor initiation/progression, immune suppression, surveillance, metabolic reprogramming, angiogenesis, the polarization macrophages. In this work, we summarize mechanisms exosome biogenesis secretion. Since exosomes may be increased cancer cells fluids patients, exosomal contents can used as diagnostic prognostic markers. contain proteins, lipids, nucleic acids. These transferred into recipient cells. Therefore, work details intercellular communications. mediate cellular interactions, targeted for developing anticancer therapy. This review summarizes current studies on effects inhibitors initiation progression. transferred, modified to deliver molecular cargo such drugs, small interfering RNAs (siRNAs), micro (miRNAs). Thus, also recent advances drug delivery platforms. display low toxicity, biodegradability, efficient tissue targeting, which make them reliable vehicles. We discuss applications challenges vehicles tumors, along with clinical values exosomes. review, aim highlight biogenesis, functions, therapeutic implications cancer.

Language: Английский

Citations

16
Standardising the preanalytical reporting of biospecimens to improve reproducibility in extracellular vesicle research – A GEIVEX study DOI Creative Commons
José Antonio López‐Guerrero, Mar Valés‐Gómez, Francesc E. Borràs

et al.

Journal of Extracellular Biology, Journal Year: 2023, Volume and Issue: 2(4)

Published: March 31, 2023

Abstract The standardization of clinical studies using extracellular vesicles (EVs) has mainly focused on the procedures employed for their isolation and characterization; however, preanalytical aspects sample collection, handling storage also significantly impact reproducibility results. We conducted an online survey based SPREC (Standard PREanalytical Code) among members GEIVEX (Grupo Español de Investigación en Vesiculas Extracelulares) to explore how different laboratories handled fluid biospecimens destined EV analyses. received 70 surveys from forty‐three laboratories: 44% plasma, 9% serum 16% urine. indicated that variability in approaches reaches 94%. Moreover, some cases, researchers had no access all relevant details samples, with potential isolation/characterisation not coded within current version SPREC. Our study highlights importance working common standard operating (SOP) control conditions. application represents a suitable approach codify register Integrating into SOPs laboratories/biobanks will provide valuable source information constitute advance research by improving credibility.

Language: Английский

Citations

14
Extracellular vesicles: powerful candidates in nano-drug delivery systems DOI
Xiaofei Liu,

Yinfang Cao,

Shuming Wang

et al.

Drug Delivery and Translational Research, Journal Year: 2023, Volume and Issue: 14(2), P. 295 - 311

Published: Aug. 15, 2023

Language: Английский

Citations

12
Revolutionizing cancer treatment via bioengineered extracellular vesicles: Exploring nanovesicles to fully synthetic solutions DOI Creative Commons

Minmin Shao,

Juliana Rodrigues, Inês Sousa‐Oliveira

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 40, P. 102395 - 102395

Published: Aug. 28, 2024

The prevalence of cancer on a global scale has necessitated the development various therapeutic approaches. However, effectiveness and safety existing methods often face some limitations. Nanotechnology emerged as solution, enabling design nanosystems that can effectively deliver drugs. These address challenges such low drug stability solubility, well lack tumor targetability. While nanomaterials offer advantages, certain nanoparticles have their own limitations, which prompted researchers to explore innovative delivery systems. One promising avenue is use extracellular vesicles (EVs), are natural secreted by cells. EVs possess biocompatibility, stability, targeting capabilities, making them ideal candidates for carriers. still remain in this field. include limited production yield, complexity, inefficient cargo loading, controlled release To overcome these challenges, been employing engineering techniques modify structures EVs, enhancing intrinsic properties. Surface modification hybrid systems combine with other shown potential addressing This review focuses landscape crucial role therapeutics diagnosis. It begins exploring biological functions properties EVs. Additionally, introduces strategies isolating shedding light methodologies used harvest minute entities. Finally, highlights biomedical applications bioengineering treatment.

Language: Английский

Citations

4