Comparative Assessment of sEV Biogenesis Regulator Proteins in MSCs and HeLa Cells for Elucidating Novel Therapeutic Strategies DOI Creative Commons
Yashvi Sharma, Sujata Mohanty

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract In the intricate landscape of cellular communication, small extracellular vesicles (sEV) originating from endosomes play crucial roles as mediators and have garnered significant attention in theranostics. Our understanding sEV biogenesis largely stems studies on cancer cells, which are vital for diagnostics. However, therapeutics, where mesenchymal stem cell (MSC)-derived emerging investigational new drugs, their pathways remain unexplored. This article explores parallel narratives cells specifically using HeLa MSCs model lines. study investigated key proteins—HRS, STAM, TSG101, ALIX—as identified context MSC-sEV biogenesis. While these proteins show similarities across types, a discernible difference arises primary functions regulating The critical role ALIX MSC biogenesis, particular, underscores its potential target modulating yield regenerative therapies. Through this comparative analysis, we shared molecular signatures, offering insights to guide therapeutic interventions unlock cells.

Language: Английский

Harnessing Nature’s Defence: The Antimicrobial Efficacy of Pasteurised Cattle Milk-Derived Extracellular Vesicles on Staphylococcus aureus ATCC 25923 DOI Open Access
Dulmini Nanayakkara Sapugahawatte,

Kasun Godakumara,

Mihkel Mäesaar

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4759 - 4759

Published: April 26, 2024

Increasing antimicrobial resistance (AMR) challenges conventional antibiotics, prompting the search for alternatives. Extracellular vesicles (EVs) from pasteurised cattle milk offer promise, due to their unique properties. This study investigates efficacy against five pathogenic bacteria, including Staphylococcus aureus ATCC 25923, aiming combat AMR and develop new therapies. EVs were characterised tested using various methods. Co-culture experiments with S. showed significant growth inhibition, colony-forming units decreasing 2.4 × 105 CFU/mL (single dose) 7.4 104 (triple doses) after 12 h. Milk extended lag time (6 9 h) increased generation (2.8 4.8 dose-dependently, compared controls. In conclusion, exhibit dose-dependent inhibition aureus, prolonging times. Despite limitations, this suggests potential in addressing AMR.

Language: Английский

Citations

1
Metabolic profiling of urinary exosomes for systemic lupus erythematosus discrimination based on HPL-SEC/MALDI-TOF MS DOI

Shaohan Yan,

Zhongzhou Huang, Xiaofei Chen

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2023, Volume and Issue: 415(26), P. 6411 - 6420

Published: Aug. 30, 2023

Language: Английский

Citations

2
Effect of homotypicvs. heterotypic interactions on the cellular uptake of extracellular vesicles DOI Open Access

Jhanvi R. Jhaveri,

Purva Khare,

Paromita Paul Pinky

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 25, 2023

Abstract Extracellular vehicles ( EVs ) are an emerging class of drug carriers and primarily reported to be internalized into recipient cells via a combination endocytic routes such as clathrin-mediated, caveolae-mediated macropinocytosis pathways. In this work, 1 we investigated potential effects homotypic vs . heterotypic interactions by studying the cellular uptake homologous (EV donor same type) heterologous different types) 2 determined route EV internalization low pinocytic/hard-to-deliver cell models brain endothelial BECs ). We used macrophages low-pinocytic phagocytic models, respectively, study effect on in cells. Homotypic led greater extent compared interactions. However, did not see complete reduction when pathways were blocked using pharmacological inhibitors. Our results suggest that use membrane fusion enter instead relying endocytosis. Lipophilic PKH67 dye-labeled but intravesicular esterase-activated calcein ester-labeled severely reduced particle while both types EV-labeled particles comparable extents. also highlight importance carefully choosing labeling dye chemistry uptake, especially case pinocytic BECs. Graphical abstract

Language: Английский

Citations

1
Comparative Assessment of sEV Biogenesis Regulator Proteins in MSCs and HeLa Cells for Elucidating Novel Therapeutic Strategies DOI Creative Commons
Yashvi Sharma, Sujata Mohanty

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract In the intricate landscape of cellular communication, small extracellular vesicles (sEV) originating from endosomes play crucial roles as mediators and have garnered significant attention in theranostics. Our understanding sEV biogenesis largely stems studies on cancer cells, which are vital for diagnostics. However, therapeutics, where mesenchymal stem cell (MSC)-derived emerging investigational new drugs, their pathways remain unexplored. This article explores parallel narratives cells specifically using HeLa MSCs model lines. study investigated key proteins—HRS, STAM, TSG101, ALIX—as identified context MSC-sEV biogenesis. While these proteins show similarities across types, a discernible difference arises primary functions regulating The critical role ALIX MSC biogenesis, particular, underscores its potential target modulating yield regenerative therapies. Through this comparative analysis, we shared molecular signatures, offering insights to guide therapeutic interventions unlock cells.

Language: Английский

Citations

0