Efficient polymalic acid production from corn straw hydrolysate by detoxification of phenolic inhibitors DOI Creative Commons
Jun Xia,

Zhongyang Qiu,

Shibiao Ma

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Dec. 13, 2023

Inhibitory compounds generated from lignocellulose pretreatment would inhibit Poly (malic acid) (PMA) production by Aureobasidium pullulans, but the tolerance mechanism of A. pullulans to lignocellulosic inhibitor is poorly understood. In this study, cellular response stress was studied. Among three groups inhibitors (furans, weak acids and phenolic aldehydes), aldehyde dominant for PMA production. Phenolic mainly converted into alcohol also exhibited severe inhibition on Furthermore, effect detoxification methods inhibitor-removal fermentation investigated, both CaCO3 overliming presented poor effect, whereas resin H103 could remove furan derivatives efficiently, thereby producing 26.27 g/L with a yield 0.30 g/g in batch fermentation. This study will be beneficial development biomass.

Language: Английский

Bioethanol Production from Woody Biomass: Recent Advances on the Effect of Pretreatments on the Bioconversion Process and Energy Yield Aspects DOI Creative Commons
Maria El Hage, Nicolas Louka, Sid-Ahmed Rezzoug

et al.

Energies, Journal Year: 2023, Volume and Issue: 16(13), P. 5052 - 5052

Published: June 29, 2023

As greenhouse gas emissions are continuously increasing, research is now privileging greener and more sustainable human activities. An attractive strategy in the pursuit of sustainability valorization lignocellulosic biomasses for production bioethanol. This approach relies on bioconversion wood agricultural waste, which abundant globally. They represent considerable sources fermentable sugars that can be recovered through enzymatic hydrolysis. However, presence lignin waste makes it recalcitrant to hydrolysis, reduces efficiency process. Therefore, a pretreatment preceding hydrolysis highly necessary order disrupt resistant structure woody biomass. The type severity affect outcomes fermentation steps, just as they strongly influence overall process costs. Given this context, bioenergy from biomass promising alternative method sustainably responding energy demands while reducing amounts left nature. aim review thoroughly compare recent processes performed substrates over past five years, with focus thermomechanical pretreatments. Moreover, will discuss their separate impact process, energetic aspects.

Language: Английский

Citations

19
Isopropanol production using engineered Corynebacterium glutamicum from waste rice straw biomass DOI
Xiaoyu Shi,

Joonhee Chang,

Minhye Kim

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 396, P. 130416 - 130416

Published: Feb. 3, 2024

Language: Английский

Citations

7
Enhanced upgrading of lignocellulosic substrates by coculture of Saccharomyces cerevisiae and Acinetobacter baylyi ADP1 DOI Creative Commons
Changshuo Liu, Bo-Hyun Choi,

Elena Efimova

et al.

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: May 6, 2024

Abstract Background Lignocellulosic biomass as feedstock has a huge potential for biochemical production. Still, efficient utilization of hydrolysates derived from lignocellulose is challenged by their complex and heterogeneous composition the presence inhibitory compounds, such furan aldehydes. Using microbial consortia where two specialized microbes complement each other could serve approach to improve efficiency lignocellulosic upgrading. Results This study describes simultaneous inhibitor detoxification production lactic acid wax esters synthetic hydrolysate defined coculture engineered Saccharomyces cerevisiae Acinetobacter baylyi ADP1. A. ADP1 showed bioconversion aldehydes present in hydrolysate, namely furfural 5-hydroxymethylfurfural, did not compete substrates with S. , highlighting its partner. Furthermore, remaining carbon sources byproducts were directed ester The productivity was improved approximately 1.5-fold (to 0.41 ± 0.08 g/L/h) ADP1, compared monoculture . Conclusion yeast bacterium shown consumption hydrolysate. high capacity ability produce high-value products demonstrates strain be candidate increase economics fermentations.

Language: Английский

Citations

6
Applications of Brewer’s Spent Grain Hemicelluloses in Biorefineries: Extraction and Value-Added Product Obtention DOI Open Access
Aline Ruth Schmidt, Aline Perin Dresch, Sérgio L. Alves

et al.

Catalysts, Journal Year: 2023, Volume and Issue: 13(4), P. 755 - 755

Published: April 15, 2023

A circular economy is imperative for environmental sustainability. In this context, biorefineries stand out as a means of production able to reduce the carbon footprint and impact global warming. Biorefineries may employ lignocellulosic biomass from various plant sources produce bioproducts with potential replace fossil derivatives through synthesis by microorganisms without competing food crops. Brewer’s spent grain (BSG), residue brewery process, an option use, being cheap raw material highly available throughout year. The chemical composition quite variable, significant amounts hemicellulose, mainly consisting xylose arabinose monomers that can be technologically converted into value-added products such xylooligosaccharides, xylitol, second-generation ethanol (2G ethanol), biofilms furfural. To end, catalysts are unusual in making increasingly competitive market, selectively optimizing reactions reducing processes these bioproducts. present review addresses primary methods extracting processing hemicelluloses BSG using either biocatalysts (enzymes) or homogenous (acids, alkali, salts) heterogenous (solid acids metal oxide) used pretreat obtain preferred byproducts. state art optimized catalysis mechanisms also presented.

Language: Английский

Citations

13
Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation DOI Creative Commons
Barbora Branská,

Kamila Koppova,

Markéta Husáková

et al.

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: June 25, 2024

Abstract Background Inhibitors that are released from lignocellulose biomass during its treatment represent one of the major bottlenecks hindering massive utilization in biotechnological production chemicals. This study demonstrates negative effect inhibitors can be mitigated by proper feeding strategy. Both, crude undetoxified hydrolysate and complex medium supplemented with corresponding were tested acetone–butanol–ethanol (ABE) fermentation using Clostridium beijerinckii NRRL B-598 as producer strain. Results First, it was found sensitivity C. to varied different growth stages, being most significant early acidogenic phase less pronounced late acidogenesis solventogenesis. Thus, a fed-batch regime three schemes for toxic (no batch mode observed). The best results obtained when an otherwise initiated close metabolic switch, resulting stable high ABE production. Complete glucose, up 88% xylose, obtained. abundant present alkaline wheat straw ferulic coumaric acids; both phenolic acids efficiently detoxified intrinsic activity clostridia stages cultivation well period, thus preventing their accumulation. Finally, strategy verified TYA culture inhibitors, 500% increase butanol titer over control which added prior inoculation. Conclusion Properly timed sequential effectively prevented acid-crash enabled substrate. unequivocally appropriate process fully eliminate effects lignocellulose-derived inhibitors. Graphical

Language: Английский

Citations

4
Municipal green waste as substrate for the microbial production of platform chemicals DOI Creative Commons

Marianne Volkmar,

Anna-Lena Maus,

Martin Weisbrodt

et al.

Bioresources and Bioprocessing, Journal Year: 2023, Volume and Issue: 10(1)

Published: July 22, 2023

In Germany alone, more than 5·10

Language: Английский

Citations

11
Advantages of using biofilms to obtain high-value molecules by microbial biotransformations DOI Creative Commons
Nicoly Subtil de Oliveira, Romeu Cassiano Pucci da Silva Ramos, Rafaela Caldas de Paula

et al.

Published: Jan. 14, 2025

Microbial biotransformations are valuable tools from “green chemistry” and involve converting parental molecules into new daughter ones with unique physical, chemical, or pharmacological properties. These reactions often carried out by cells grown under a planktonic phenotype. However, microbial phenotype of biofilm can improve biotransformation bioprocesses once they form more biomass per volume, resistant to extreme conditions (pH, temperature, toxic substances), remain active for extended periods, less prone cell washouts, reduce re-inoculation demands, leading increased production rates due their physiological features. In addition, experience has shown that biofilms may furnish broader array molecules. This review highlighted the benefits using in obtain variety bioactives.

Language: Английский

Citations

0
Metabolic engineering strategies for food additive ferulic acid production DOI
Mei Li, Qiang Li, Jung-Kul Lee

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: 65, P. 106108 - 106108

Published: Feb. 10, 2025

Language: Английский

Citations

0
Diluted Acid Hydrolysate of Olive Stones: Overliming and Biomass Fermentation DOI Creative Commons
Jeanne Andary, Naïm Ouaini, Rony Abou‐Khalil

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(2), P. 100 - 100

Published: Feb. 17, 2025

To valorize olive stones, this study focuses on the composition of their dilute-acid hydrolysate DAH and aims to highlight effect overliming process achieve an effective treatment that maximizes sugar concentrations while minimizing number toxic materials. The examined impact pH (10 12), temperature (25 60 °C), detoxification time (15, 30, min) viability vitality M. pulcherrima S. cerevisiae, using experimental design 2231. Detoxification was significantly influenced by temperature, with xylose furans probably following same kinetic degradation. Viability improved 52% for 67% cerevisiae in detoxified due reduced compounds. Optimal conditions were found be a 10 at 25 °C 30 min, achieving 71% 62% degradation hydroxymethylfurfural furfural, respectively, minimum polyphenol concentration 580 mg·L−1. pulcherima exhibited greater than because medium’s high content low glucose concentration. Conversely, 12 not only promoted (xylose) loss but also generated new compounds negatively affected yeast development. improve fermentation, further attention needs paid these conditions.

Language: Английский

Citations

0
Biodegradation of T‐2 Toxin by a Dominant Microbial Consortium Isolated From Chinese Yeast Ball DOI Creative Commons

Chun‐Ming Yang,

Norlia Mahror, Shao‐Ji Li

et al.

eFood, Journal Year: 2025, Volume and Issue: 6(2)

Published: March 12, 2025

ABSTRACT T‐2 toxin is a highly toxic type A trichothecene mycotoxin commonly found in feed, agricultural products like wheat, corn, and barley, as well processed foods, presenting substantial health hazards to both humans animals. In this study, microbial consortium (designated BJ) with stable efficient degradation activity was isolated from Chinese yeast balls, traditional fermentation starter. The dominated by species of Wickerhamomyces , Pichia Pediococcus . This BJ demonstrated the ability degrade 99.2% HT‐2 toxin, triol, tetraol within 48 h, outperforming individual strains. Metagenomic sequencing LC‐MS/MS metabolic analysis elucidated pathway, which includes deacetylation de‐epoxidation epoxy group, cytochrome P450 enzyme CYP61 ciferrii playing key role detoxification. study highlights potential natural agent for detoxification feed food industry, though further research warranted assess its real‐world application safety.

Language: Английский

Citations

0