The Neuroscience Journal of Shefaye Khatam, Journal Year: 2023, Volume and Issue: 12(1), P. 111 - 120
Published: Dec. 1, 2023
Language: Английский
AIMS neuroscience, Journal Year: 2023, Volume and Issue: 10(4), P. 332 - 353
Published: Jan. 1, 2023
<abstract> <p>Spinal cord injury (SCI) is a debilitating condition that results in impaired sensory and motor function due to the limited self-regenerative ability of spinal cord. To address this issue, combination therapy has been proposed as an effective treatment strategy for SCI regeneration. In study, Platelet-Rich Plasma (PRP)-derived exosomes loaded with dexamethasone were utilized mouse model compression. PRP-derived (Dex) prepared using ultracentrifugation sonication methods administered mice via intravenous injection. Following four-week duration, behavioral assessments assess functional recuperation, diverse metrics encompassing expression genes associated apoptosis antiapoptosis, serum cytokine concentrations tissue sampling subjected thorough examination. The study demonstrated treated Dex (ExoDex) exhibited altered levels TNF-α IL-10, along decreased Bax increased Bcl2 comparison group. Furthermore, intravenously injected ExoDex reduced size lesion site, lymphocyte infiltration, vacuolation, cavity disorganization while also improving locomotor recovery. We propose utilization exosome-loaded holds potential promising clinically relevant approach injured repair. However, further extensive research warranted domain validate substantiate outcomes presented study.</p> </abstract>
Language: Английский
Citations
7
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: March 14, 2024
Mutations in human VPS4A are associated with neurodevelopmental defects, including motor delays and defective muscle tone. encodes a AAA-ATPase that is required for membrane scission, but how mutations lead to impaired control of function not known. Here we identified mutation zebrafish vps4a , T248I, affects sensorimotor transformation. In biochemical experiments show the T248I reduces ATPase activity Vps4a disassembly its substrate, ESCRT filaments, which mediate scission. Consistent established role endocytic pathway exosome biogenesis, mutants have enlarged endosomal compartments CNS decreased numbers circulating exosomes. Resembling central form hypotonia patients, neurons cells unaffected mutant as touch sensitivity intact. Unlike somatosensory function, optomotor responses, vestibulospinal (VS), acoustic startle reflexes severely mutants, indicating greater these circuits mutation. ERG recordings indicate visual ability largely reduced however, vivo imaging tone-evoked responses inner ear ascending auditory comparable activity. Further investigation pathways revealed sensory cues failed fully activate VS medial longitudinal fasciculus (MLF) nuclei directly innervate neurons. Our results suggest defect transformation underlies profound yet selective effects on resulting from loss scission mediated by Vps4a.
Language: Английский
Citations
2
Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17
Published: Dec. 14, 2023
Spinal cord injury (SCI) is a critical neurological condition that may impair motor, sensory, and autonomous functions. At the cellular level, inflammation, impairment of axonal regeneration, neuronal death are responsible for SCI-related complications. Regarding high mortality morbidity rates associated with SCI, there need effective treatment. Despite advances in SCI repair, an optimal treatment complete recovery after has not been found so far. Therefore, strategy needed to promote regeneration repair SCI. In recent years, regenerative treatments have become potential option achieving improved functional by promoting growth new neurons, protecting surviving preventing additional damage spinal cord. Transplantation cells cells-derived extracellular vesicles (EVs) can be recovery. However, some limitations challenges related cell-based strategies. Ethical concerns limited efficacy due low survival rate, immune rejection, tumor formation therapies. Using EVs helpful overcome these limitations. It should considered short half-life, poor accumulation, rapid clearance, difficulty targeting specific tissues EVs-based Hydrogel-encapsulated exosomes enhancing through maintaining their bioactivity, from facilitating sustained release at target site. These hydrogel-encapsulated neuroregeneration improving recovery, reducing This review aims provide overview current research status, challenges, future clinical opportunities
Language: Английский
Citations
6
Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101250 - 101250
Published: Sept. 14, 2024
Language: Английский
Citations
1
Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(6), P. 5180 - 5197
Published: June 16, 2023
Spinal cord injury (SCI) is a destructive condition that results in lasting neurological damage resulting disruption of the connection between central nervous system and rest body. Currently, there are several approaches treatment damaged spinal cord; however, none methods allow patient to return original full-featured state life before injury. Cell transplantation therapies show great potential cords. The most examined type cells used SCI research mesenchymal stromal (MSCs). These at center interest scientists because their unique properties. MSCs regenerate injured tissue two ways: (i) they able differentiate into some types so can replace (ii) through powerful known paracrine effect. This review presents information about treatments usually used, aiming cell therapy using products, among which active biomolecules extracellular vesicles predominate.
Language: Английский
Citations
2
PubMed, Journal Year: 2024, Volume and Issue: 15(1), P. 27 - 34
Published: Jan. 1, 2024
Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin nutrition consumption decreases. Currently, it is proposed to measure glucose content in human or animal subjects predict recovery rate efficiency treatments following SCI. In this study, effect extra-cellular vesicles (EVs) from immortalized adipose tissue-derived mesenchymal stem cells (hTERT-MSCs) equipped with ectopic expression
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0
Highlights in Science Engineering and Technology, Journal Year: 2024, Volume and Issue: 123, P. 527 - 533
Published: Dec. 24, 2024
With the advancement of spinal cord injury (SCI) research, stem cell therapy has garnered increasing attention. However, field encompasses numerous research topics and branches, necessitating further organization focus. This review aims to provide a coherent summary mechanisms roles cells in SCI treatment, addressing three key aspects: role macrophage polarization SCI, regulatory by which cells, specifically mesenchymal (MSCs) neural (NSCs), influence polarization, signaling pathways involved cell-regulated polarization. Correct is crucial for effective with MSCs NSCs contributing through chemical mediators, modulation interactions pathways. Ultimately, will explore how these enhance treatment diverse forms signal transduction regulation. In conclusion, this summarize current insights into integrating interconnected aspects discussed.
Language: Английский
Citations
0
The Neuroscience Journal of Shefaye Khatam, Journal Year: 2023, Volume and Issue: 12(1), P. 111 - 120
Published: Dec. 1, 2023
Language: Английский
Citations
0