Platinum Wire-embedded Culturing Device for Interior Recording from Lollipop-shaped Neural Spheroids

Cyborg and Bionic Systems, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Three-dimensional (3D) neural cultures are increasingly recognized for their complexity and resemblance to in vivo microenvironments. In this paper, we present a novel 3D cell culturing noninvasive characterization technique of spheroids. Based on embedded platinum wires, the cultured cells lollipop-shaped spheroids where axons extended integrated around wires. Electrical microstimulation enhanced connectivity between demonstrated signal propagation among them. The resultant axonal elongation facilitated formation robust tracts interconnecting Variation cells' density allows adjust spheroid's diameter, identifying 1 million as good number spheroid formation. Recordings activities reveal higher-quality measurement from interior compared those obtained exterior cells. Viability assays confirmed efficacy proposed sustained growth over 1-month period. holds potential applications various fields, such development brain organoids, which enables real-time interconnection sensing environment conditions.

Language: Английский

---

Advancing precision medicine: strategies from organoids research

Cell organoid (Print), Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

---

Brain organoids-on-chip for neural diseases modeling: History, challenges and trends

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101323 - 101323

Published: April 1, 2025

Language: Английский

---

Carbon Nanotube-Based Printed All-Organic Microelectrode Arrays for Neural Stimulation and Recording

Micromachines, Journal Year: 2024, Volume and Issue: 15(5), P. 650 - 650

Published: May 14, 2024

In this paper, we report a low-cost printing process of carbon nanotube (CNT)-based, all-organic microelectrode arrays (MEAs) suitable for in vitro neural stimulation and recording. Conventional MEAs have been mainly composed expensive metals manufactured through high-cost complex lithographic processes, which limited their accessibility neuroscience experiments application various studies. Here, demonstrate printing-based fabrication method microelectrodes using organic CNT/paraffin ink, coupled with the deposition an insulating layer featuring single-cell-sized sensing apertures. The simple microfabrication processes utilizing economic readily available ink offer potential cost reduction improved MEAs. Biocompatibility fabricated was suggested live/dead assay cultured cells, its large electric double capacitance revealed by cyclic voltammetry that crucial preventing cytotoxic electrolysis during stimulation. Furthermore, electrode exhibited sufficiently low impedance 2.49 Ω·cm2 high signal-to-noise ratio recording, successfully captured model waves physiological saline solution. These results suggest easily producible printed are believe they can expand MEA research.

Language: Английский

---

Highly efficient combination of multiple single cells using deterministic single-cell combinatorial reactor

Lab on a Chip, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

This study presents a novel microfluidic device coupled with multilayer interconnect Si/SiO 2 control circuit, named the deterministic single-cell combinatorial reactor, for highly efficient combination of multiple single cells.

Language: Английский

---