Rapid, sensitive, and visual detection of swine Japanese encephalitis virus with a one-pot RPA-CRISPR/EsCas13d-based dual readout portable platform

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134151 - 134151

Published: July 24, 2024

Language: Английский

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CRISPR/Cas13a-mediated visual detection: A rapid and robust method for early detection of Nosema bombycis in silkworms

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 175, P. 104203 - 104203

Published: Oct. 20, 2024

Language: Английский

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CRISPR-Cas12a based detection of EGFR gene mutation in cell free DNA for early diagnosis of non-small cell lung cancer (NSCLC)

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100735 - 100735

Published: Jan. 1, 2025

Language: Английский

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Emerging microfluidic technologies for CRISPR-based diagnostics: an overview

Analytical Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review explores emerging CRISPR-microfluidic platforms enhancing precision, speed, and portability for point-of-care diagnostics. Innovations like SHINE, CARMEN, ITP, DNAiTECH, Dμchip, FAST, MAPnavi highlight their potential.

Language: Английский

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RAA-CRISPR/Cas13a-assisted lateral flow strip for Feline herpesvirus Type I point-of-care testing

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113135 - 113135

Published: Feb. 1, 2025

Language: Английский

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Ultrasensitive rapid quantitative CRISPR-based detection of cervical cancer tumor marker proteins SCCA and CEA

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162465 - 162465

Published: April 1, 2025

Language: Английский

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One‐Step RAA and CRISPR‐Cas13a Method for Detecting Influenza B Virus

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(4)

Published: April 1, 2025

ABSTRACT We developed a sensitive and specific method based on recombinase‐aided amplification (RAA) clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated protein 13a (Cas13a). This method, named CRISPR‐based Rapid Efficient Test (CRISPRET), is designed for the early diagnosis of Influenza B (FluB) with aim shortening its transmission chain. identified conserved regions in Virus (IBV) NS gene forward reverse primers along crRNAs. then established optimised reaction system, Nucleic Acid Positive Reference Materials IBV were used to evaluate detection limit (DL) CRISPRET. Additionally, we collected 257 clinical samples, comprising 127 samples from patients infection 130 healthy individuals, subjected them dual using CRISPRET qPCR positive predictive value (PPV), negative (NPV), sensitivity specificity one primer, two primers, crRNAs establish optimise CRISPR ET. The demonstrated DL 500 copies·μL −1 when assisted by appropriate equipment. Despite requiring auxiliary equipment 30‐min reaction, ET enables nucleic acid within approximately first 5 min, achieving high (100%), (97.69%), PPV (97.69%) NPV concordance rate 98.83% qPCR. offers simple, field‐applicable, one‐step rapid IBV. It has strong potential field‐testing applications intelligent integration into existing diagnostic systems.

Language: Английский

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Online synthesis of crRNA for One-Step RPA-CRISPR-Cas12 assay of Group b Streptococcus

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113745 - 113745

Published: April 1, 2025

Language: Английский

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Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a

Experimental Biology and Medicine, Journal Year: 2025, Volume and Issue: 250

Published: May 1, 2025

Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A RSV-B, particularly in resource-limited settings. The limit for RSV-B was approximately 102 103 copies/reaction, respectively. assay revealed 100% specificity detecting both RSV-B. Diagnostic accuracy 90.32 93.55% respectively, compared RT-qPCR. These data indicate a proficient strategy RSV screening, demonstrating promise prospective applications diverse viral infections.

Language: Английский

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Establishment and evaluation of a naked-eye diagnostic assay for tuberculosis utilizing reverse isothermal amplification-assisted CRISPR-Cas in resource-limited settings

Drug Target Insights, Journal Year: 2025, Volume and Issue: 19(1), P. 31 - 40

Published: May 9, 2025

Introduction: The current scenario of tuberculosis (TB) caused by Mycobacterium (MTB) has presented an almost insurmountable challenge to hospitals with high patient numbers. Delayed diagnosis TB is a major hurdle in preventing the employment efficient therapeutics, leading development drug resistance. Hence, easily accessible diagnostic method, particularly for resource resource-limited settings, pertinent rapid identification MTB-infected patients. In pursuit developing such assay, present study offers CLAP-TB (CRISPR-Cas coupled RT-LAMP Amplification Protocol Tuberculosis) which will allow us diagnose rapidly and visually. Methods results: Herein, visual MTB detection consists method utilizing 232 different samples (sputum, urine, serum) from 82 patients reverse transcription loop-mediated isothermal amplification (RT-LAMP). Additionally, assay also utilizes integration CRISPR-Cas12-based system using guide RNAs IS6110 internal control POP7 (human RNase P) genes along via lateral flow readoutbased dipsticks unaided eye (~134 min). Overall, limit was up 1 ag RNA, while clinical sensitivity specificity were 98.27% 100%, respectively, on pilot scale.Conclusion: Together, our proof concept rapid, sensitive, specific minimum technical expertise required settings.

Language: Английский

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