Fluorinated Porcine Bone-Derived Hydroxyapatite Promotes Vascularized Osteogenesis by Coordinating Human Bone Marrow Mesenchymal Stem Cell/Human Umbilical Vein Endothelial Cell Complexes DOI Creative Commons
Xiayi Wu,

Chunxin Xu,

Junming Feng

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(12), P. 1287 - 1287

Published: Dec. 18, 2024

Biogenic hydroxyapatite is known for its osteoinductive potential due to similarity human bone and biocompatibility, but insufficient vascularization compared autogenous during early implantation limits integration osteogenesis. Fluorine has been shown improve hydroxyapatite’s mechanical properties the coupling of osteogenic angiogenic cells. In this study, fluorine-modified biogenic (FPHA) with varying fluorine concentrations was prepared tested ability promote vascularized FPHA in study retained natural porous structure biological cancellous released F− ions when immersed cell culture medium. The extraction solutions FPHA0.25 FPHA0.50 promoted formation capillary-like tubes by umbilical vein endothelial cells (HUVECs), significantly upregulating vegf mRNA VEGF protein levels co-cultured marrow mesenchymal stem (HBMSCs). Additionally, upregulated pdgf-bb PDGF-BB HUVECs. vivo experiments using a rabbit cranial defect model demonstrated that angiogenesis area, enhanced secretion, increased PDGFR-β expression These findings suggest an optimal concentration (FPHA0.25) may offer promising strategy enhance body’s innate bone-healing accelerating vascularization.

Language: Английский

Nanofibrous Microspheres: A Biomimetic Platform for Bone Tissue Regeneration DOI Creative Commons
Nimeet Desai, Shreya Pande, Lalitkumar K. Vora

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(7), P. 4270 - 4292

Published: July 1, 2024

Bone, a fundamental constituent of the human body, is vital scaffold for support, protection, and locomotion, underscoring its pivotal role in maintaining skeletal integrity overall functionality. However, factors such as trauma, disease, or aging can compromise bone structure, necessitating effective strategies regeneration. Traditional approaches often lack biomimetic environments conducive to efficient tissue repair. Nanofibrous microspheres (NFMS) present promising platform regeneration by mimicking native extracellular matrix architecture. Through optimized fabrication techniques incorporation active biomolecular components, NFMS precisely replicate nanostructure biochemical cues essential osteogenesis promotion. Furthermore, exhibit versatile properties, including tunable morphology, mechanical strength, controlled release kinetics, augmenting their suitability tailored engineering applications. enhance cell recruitment, attachment, proliferation, while promoting osteogenic differentiation mineralization, thereby accelerating healing. This review highlights engineering, elucidating design principles key attributes. By examining recent preclinical applications, we assess current clinical status discuss critical considerations potential translation. offers crucial insights researchers at intersection biomaterials highlighting developments this expanding field.

Language: Английский

Citations

4
Bone Tissue Engineering: From Biomaterials to Clinical Trials DOI

Swapnali Jagadale,

Mrunal N. Damle, Meghnad G. Joshi

et al.

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Icariside II enhances crania defect repair through synergistic angiogenesis and osteogenesis DOI

Zhangshun Yao,

Weixiang Huang,

Jingxian Jiang

et al.

Tissue and Cell, Journal Year: 2025, Volume and Issue: 95, P. 102833 - 102833

Published: March 7, 2025

Language: Английский

Citations

0
From Bone To Blood Flow: Tissue Engineering In Orthopedics – A Narrative Review DOI Creative Commons
Bshara Sleem,

Rakan Nassereldine,

Victor Ghazi

et al.

Orthopedic Reviews, Journal Year: 2025, Volume and Issue: 17

Published: March 31, 2025

Musculoskeletal injuries and degenerative conditions necessitate advanced regenerative solutions. Tissue engineering has emerged as a pivotal field in orthopedic care, particularly vascularized bone cartilage regeneration. This narrative review examines the latest advancements vascular tissue engineering, including scaffold design, cell-based techniques, growth factor delivery. A comprehensive literature search was conducted using PubMed, ScienceDirect, Google Scholar, focusing on innovations challenges field. Vascularized grafts (VBGs) outperform non-vascularized counterparts promoting healing integration. Advances materials, such smart scaffolds hybrid biomaterials, enhance osteogenesis angiogenesis. Cellular therapies, utilizing mesenchymal stem cells induced pluripotent cells, synergistically improve vascularization Growth factors like VEGF morphogenic protein (BMP-2), integrated with innovative delivery systems, enable sustained angiogenic stimulation While significant strides have been made, persist achieving full integration replicating native architecture. Innovations technology surgery techniques hold promise for transforming improving patient outcomes.

Language: Английский

Citations

0
Sequential Angiogenic-Osteogenic Coupling via a Spatiotemporally Graded Hydrogel Enables Vascularized Bone Organoids for Critical-Sized Calvarial Defect Reconstruction DOI

Xu Lou,

Fuxiao Wang,

Xia Lv

et al.

Composites Part B Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 112553 - 112553

Published: April 1, 2025

Language: Английский

Citations

0
A Multifunctional PEEK Composite Scaffold with Immunomodulatory, Angiogenic, and Osteogenic Properties for Enhanced Bone Regeneration DOI Open Access

Mengen Zhao,

Han Yang,

Qianwen Yang

et al.

Polymers, Journal Year: 2025, Volume and Issue: 17(9), P. 1206 - 1206

Published: April 28, 2025

Polyetheretherketone (PEEK) is a widely used material in bone tissue engineering due to its favorable mechanical properties and radiolucency. However, bioinert nature lack of osteogenic activity restrict ability support effective regeneration. In this study, novel APS-coated plasma-treated sulfonated bioactive PEEK scaffold (APS/PSBPK) was developed overcome these limitations. The integrates strontium-doped glass (SrBG) enhance biocompatibility potential, while astragalus polysaccharide (APS) incorporated via plasma cleaning modulate immune responses promote vascularization. vitro studies demonstrated that the APS/PSBPK facilitates M2 macrophage polarization, reduces pro-inflammatory cytokines, enhances secretion anti-inflammatory factors. It also promotes endothelial cell migration angiogenesis supporting adhesion, proliferation, differentiation rBMSCs. vivo experiments revealed effectively regulates microenvironment, vascularization, accelerates Thus, composite serves as multifunctional biomaterial with significant potential for applications repair regeneration by combining immunomodulation, angiogenesis, osteogenesis.

Language: Английский

Citations

0
An engineered M2 macrophage-derived exosomes-loaded electrospun biomimetic periosteum promotes cell recruitment, immunoregulation, and angiogenesis in bone regeneration DOI

Zhuohao Wen,

Shuyi Li, Yi Liu

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 50, P. 95 - 115

Published: April 7, 2025

Language: Английский

Citations

0
Magnetron Sputtering Dual‐Ion Sequential Release Coating on PET Artificial Ligament Promotes Vascular Nerve Regeneration and Graft‐Bone Integration DOI
Wei Wu,

Yuhao Xia,

Ye Huang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Abstract Anterior cruciate ligament (ACL) injuries in sports have become increasingly prevalent, leading to the widespread adoption of polyethylene terephthalate (PET) artificial ligaments for ACL reconstruction due their superior mechanical properties. However, bio‐inertness PET presents a significant challenge graft‐bone integration, necessitating enhancement surface bioactivity. This study employed magnetron sputtering and hydrogel coating techniques introduce strontium calcium‐phosphorus, magnesium ions onto ligaments, achieving sequential release Mg, Sr ions. The scanning electron microscopy analysis confirmed uniformity stability modified material. synergistic effect from Mg/Sr‐PET has effectively promoted osteogenesis, angiogenesis, neuronal differentiation vitro. Moreover, reconstructed model using Sprague‐Dawley rats, histological staining, micro‐computed tomography, biomechanical test results indicated that group notably stimulated blood vessel nerve formation, demonstrating remarkable bone regrowth promotion. In conclusion, significantly enhanced integration accelerated rapid healing, offering valuable insights improving clinical outcomes.

Language: Английский

Citations

2
Unlocking the regenerative key: Targeting stem cell factors for bone renewal DOI Creative Commons

Gul Karima,

Hwan Kim

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Stem cell factors (SCFs) are pivotal existing in both soluble and membrane-bound forms, expressed by endothelial cells (ECs) fibroblasts throughout the body. These enhance growth, viability, migration multipotent lineages. The preferential expression of SCF arteriolar ECs indicates that arterioles create a unique microenvironment tailored to hematopoietic stem (HSCs). Insufficiency within bone marrow (BM)-derived adipose tissue results decreased their overall cellularity, affecting HSCs immediate progenitors critical for generating diverse blood maintaining microenvironment. deficiency disrupts BM function, impacting production differentiation HSCs. Additionally, deleting from adipocytes reduces lipogenesis, highlighting crucial role SCF/c-kit signaling controlling lipid accumulation. This review elucidates sources, roles, mechanisms, molecular strategies renewal, offering comprehensive overview recent advancements, challenges, future directions leveraging as key agent regenerative medicine.

Language: Английский

Citations

2
Novel asymmetrical double-layer structural adhesive hydrogels with synergetic neuroprotection and angiogenesis effect for diabetic wound healing DOI

Zhihong Su,

Wanjun Zhang, Zhiqing Mo

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 159081 - 159081

Published: Dec. 1, 2024

Language: Английский

Citations

2