Macrophages in graft-versus-host disease (GVHD): dual roles as therapeutic tools and targets

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 6, 2025

Graft-versus-host disease remains one of the most formidable barriers to complete success hematopoietic stem cell transplantation that has emerged as curative approach for many malignancies because it affects quality life and overall survival. Macrophages are among important members immune system, which perform dual roles in GVHD both therapeutic tools targets. This review epitomizes multifunctional role macrophages pathophysiology acute chronic GVHD. play an early phase their recruitment infiltration into target organs. Furthermore, they polarize two functionally different phenotypes, including M1 M2. In case GVHD, express phenotype characterized by production pro-inflammatory cytokines contribute tissue damage. contrast, tend toward M2 associated with repair tissues fibrosis. A critical balance these phenotypes is central course severity Further interactions other lymphocytes such T cells, B fibroblast further determine Macrophage interaction alloreactive cells promotes inflammation. therefore inducing injuries during Interaction macrophages, cell, fibroblast, CD4+ fibrosis and, hence, subsequent dysfunction These some insights, while several challenges remain. First, impact dominant on polarization incompletely sometimes controversial. Second, development targeted therapies able modulate macrophage function without systemic side effects area ongoing investigation. Future directions involve exploration macrophage-targeted therapies, small molecules, antibodies, nanotechnology, behavior improve patient outcomes. underlines fact a profound understanding essential developing new more effective strategies. Targeting might represent avenue decreasing incidence improving safety HSCT.

Language: Английский

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Enhancing Gut Microbiome and Metabolic Health in Mice Through Administration of Presumptive Probiotic Strain Lactiplantibacillus pentosus PE11

Nutrients, Journal Year: 2025, Volume and Issue: 17(3), P. 442 - 442

Published: Jan. 25, 2025

Background: Over the past decade, probiotics have gained increasing recognition for their health benefits to host. While most research has focused on therapeutic effects of in treatment various diseases, recent years seen a shift towards exploring role enhancing and supporting overall health. Methods: In this work, we studied novel potential probiotic strain, Lactiplantibacillus pentosus PE11, healthy mice following six-week dietary intervention. The assessment included monitoring general animals, biochemical analyses, profiling gut fecal microbial communities, gene expression analysis. Results: Our results showed that administration PE11 led changes composition microbiome, specifically an increase Firmicutes/Bacteroidetes ratio relative abundance Lachnospiraceae, Ruminococcaceae, Rikenellaceae families. Reduced Tnf elevated Zo1 were also observed cecum, pointing anti-inflammatory properties improved intestinal barrier integrity. Additionally, significant reduction triglycerides alanine aminotransferase levels—within physiological ranges—was observed, along with trend toward decreased total cholesterol levels. Conclusions: These findings suggest mice, positively influence microbiome structure metabolism, thereby

Language: Английский

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The microbiome and metabolome modulating activity of dietary cholesterol: Insight from the small and large intestine

Food & Function, Journal Year: 2025, Volume and Issue: 16(5), P. 1872 - 1887

Published: Jan. 1, 2025

Dietary cholesterol alters the composition, metabolites, and pro-inflammatory capacity of both large small intestinal microbiota.

Language: Английский

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The Effect of Alpine Herbs on the Microbiota of In Vitro Rumen Fermentation

Fermentation, Journal Year: 2025, Volume and Issue: 11(2), P. 83 - 83

Published: Feb. 7, 2025

Milk from cows grazing on alpine pastures has higher quality than milk indoor-fed cows, likely due to diet-driven differences in rumen microbiota. We assessed the effects of supplementing herbs—each varying its content fiber, protein, and polyphenol—on microbiota via vitro fermentation, comparing these a grass hay control using metagenomic sequencing. Fermentations with herbs compared had fibrolytic Prevotella lower abundances Butyrivibrio, Ruminococcaceae, Anaerovibrio, Succiniclasticum, Desulfovibrio. high starch (Alchemilla vulgaris, Gallium odoratum Sanguisorba officinalis) low, microbial diversity, while fermentations structural fibre (Sisymbrium officinale, Tanacetum vulgare, Cicerbita alpina) diversity. C. alpina, Sa. officinalis, T. vulgare that lignin showed abundance Bacteroidetes Firmicutes. protein (G. vulgare) induced Lachnospiraceae. officinalis A. vulgaris polyphenols were associated increased Streptococcus family RF-16 BS11 alpina Si. Officinale Fibrobacter succinogenes. The beta diversity between corresponded contents lignin, plant material. In conclusion, different can promote various fibrinolytic bacteria change which potential increase feed efficiency robustness reduce methane production.

Language: Английский

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Dynamic Changes in Rumen Microbial Diversity and Community Composition Within Rumen Fluid in Response to Various Storage Temperatures and Preservation Times

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(3), P. 234 - 234

Published: March 3, 2025

The aim of this study was to investigate the effects storage temperature and preservation time on microbial diversity community composition rumen fluid. Rumen fluid samples were collected from six Hu sheep fed a high-forage diet stored at −80 °C −20 for intervals 0, 7, 14, 30, 60, 120, 240 days. DNA extracted each point 16S rRNA gene sequencing evaluate composition. results showed that affected only relative abundance Proteobacteria, with no substantial impact alpha-diversity or other groups (p > 0.05), significant interaction observed between 0.05). Alpha-diversity indices such as Chao1, species, PD whole tree dynamic changes after 7 days storage, while abundances Verrucomicrobiota Christensenellaceae R-7 group, well energy metabolism metabolic pathway, exhibited alterations 14 < Notably, Patescibacteria, Rikenellaceae RC9 gut Veillonellaceae UCG-001 demonstrated Both principal coordinates analysis (PCoA) non-metric multidimensional scaling (NMDS) distinct overlaps. This suggests storing does not influence composition, whereas significantly impacts these factors, most differences emerging preservation. Consequently, it is advised in be conducted within post-collection.

Language: Английский

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Unraveling the Metabolic and Microbiome Signatures in Fecal Samples of Pregnant Women with Prenatal Depression

Metabolites, Journal Year: 2025, Volume and Issue: 15(3), P. 179 - 179

Published: March 6, 2025

Background/Objectives: Prenatal depression (PND) poses a significant threat to the health of both mother and developing fetus. Despite its increasing prevalence, pathophysiology PND is not yet fully elucidated. Methods: In this study, we aimed investigate fecal metabolites gut microbiota in patients compared healthy controls explore potential correlations between these factors. Results: Through untargeted metabolomics analysis, identified 75 significantly altered patients, which 27 were structurally annotated implicated key pathways, such as linoleic acid metabolism phenylalanine, tyrosine, tryptophan biosynthesis. Notably, two Clostridia-associated enterobacteria, unclassified_c_Clostridia unclassified_f_Lachnospiraceae, enriched group, positively correlated with tyrosine negatively multiple sulfated neurosteroids. Conclusions: Our findings underscore robust association dysbiosis metabolic disturbances PND, specific alterations noted metabolism, neurosteroid homeostasis, pathways. These dysregulated metabolites-tyrosine, neurosteroids, acid-may serve diagnostic biomarkers therapeutic targets. Moreover, their interplay provides new insights into pathophysiological mechanisms particularly highlighting role gut-brain axis signaling neuroendocrine dysregulation inflammatory responses. However, further large-scale studies animal models are required validate detailed mechanistic

Language: Английский

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Short-chain fatty acids production from maize silage: impact of stepwise hydraulic retention time reduction on microbial adaptation and process output

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: March 18, 2025

Language: Английский

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Microbial Dynamics in Seagrass Restoration: Unveiling Hidden Indicators of Ecological Success

Marine Environmental Research, Journal Year: 2025, Volume and Issue: 208, P. 107089 - 107089

Published: March 21, 2025

Language: Английский

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Synergy of Multi-Enzyme Pretreatment and Paraclostridium benzoelyticum Bioaugmentation: A Dual Strategy for Enhancing Methane Production in Dry Anaerobic Digestion of Kitchen Waste

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121671 - 121671

Published: April 1, 2025

Language: Английский

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Role of the Intestinal Microbiota in the Molecular Pathogenesis of Atypical Parkinsonian Syndromes

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3928 - 3928

Published: April 22, 2025

The role of the intestinal microbiota and its influence on neurodegenerative disorders has recently been extensively explored, especially in context Parkinson’s disease (PD). In particular, immunomodulation, impact inflammation, participation gut–brain axis are under ongoing investigations. Recent studies have revealed new data that could be important for exploring neurodegeneration mechanisms connected with gut microbiota, potentially leading to development methods treatment. this review, potential roles future disease-modifying therapies were discussed properties microbiota—including impacts metabolism short-chain fatty acids vitamins—were summarized, a particular focus atypical Parkinsonian syndromes. This review focused detailed description numerous through which influences processes. explored connections between evolution progression Finally, derived results regarding alterations syndromes comparison previously described PD was also included.

Language: Английский

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