The Lachnospiraceae-butyric acid axis and its role in glucocorticoid-associated osteonecrosis DOI Creative Commons

Mingbin Guo,

Shuai He, Wei Song

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 11, 2024

Glucocorticoids (GCs) are key inducers of osteonecrosis, yet not all patients treated with GCs develop glucocorticoid-associated osteonecrosis (GAON). The factors mediating this relationship unclear. Studies have shown that gut microbiota and their metabolites influence bone metabolism, but role in GAON is This study aimed to explore the connection between microbiota. Through bidirectional Mendelian randomization analysis, we identified 14 microbial taxa, including Lachnospiraceae (IVW, P = 0.011), associated GAON. RNA-seq analysis revealed differentially expressed genes (DEGs) were enriched for intestinal inflammatory response mechanisms. We then compared who developed (17 cases), those did (GAnON, 15 untreated (Blank, cases) composition, short-chain fatty acids (SCFAs), serum factors. Our findings indicated a decrease abundance (GAON 17.13%, GAnON 12.51%, Blank 24.52%) GC-treated patients. Serum (IL-17 A, IL-33, TNF-α) (59.603 ± 12.147, 89.337 20.714, 42.584 9.185) showed significant differences (1.446 0.683, 11.534 4.705, 4.682 1.48) (25.353 8.181, 32.527 7.352, 12.49 3.217) groups, negative correlation these levels. Butyric acid levels SCFAs varied among groups (P<0.01) correlated Controlled experiments rats demonstrated butyric acid's osteoprotective development (P<0.01). In conclusion, our suggests reduced levels, along increased inflammation due use, contribute may mediate effects inflammation. Butyrate supplementation could potentially reduce incidence, offering novel approach its clinical management.

Language: Английский

De novo anaerobic granulation with varying organic substrates: Granule growth and microbial community responses DOI Creative Commons
Chang Gao, Anna Doloman,

Emilie Alaux

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175570 - 175570

Published: Aug. 15, 2024

Anaerobic granulation from dispersed inoculum is recognized as a slow process. However, studies under saline conditions have shown that adding complex proteinaceous substrates can accelerate this To explore whether holds true also non-saline conditions, we conducted 262-days experiment with four lab-scale upflow anaerobic sludge blanket reactors inoculated digested sewage sludge. Each reactor received synthetic feed containing varying amount of carbohydrate/protein substrate: glucose (RGlu), acetate/tryptone (RAc+Try), glucose/tryptone (RGlu+Try), and glucose/starch (RGlu+Sta). Development granules different influent composition was monitored macroscopy, analysis the extracellular polymeric substances, microbial diversity. Granulation faster in RGlu+Try RGlu+Sta. Increasing granule diameters positively correlated occurrence bacteria Muribaculaceae Lachnospiraceae families, suggesting their involvement de novo granulation. Granules had high relative abundances both fermenting (e.g. Lactococcus, Streptococcus, Trichococcus) involved oxidation volatile fatty acids (Smithella, Acetobacteroides). The results study provide basis for strategies to enhance rate practice when granular not available. Specifically, supplementing small amounts waste protein during start-up be effective.

Language: Английский

Citations

0
State-Threatened Gopher Tortoise (Gopherus polyphemus) Gut Microbiome Analysis Reveals Health Insights into Southeastern Florida Population DOI Creative Commons
Dimitrios Stasi Giakoumas, Tracy J. Mincer, Kelsie M. Bernot

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

The gopher tortoise (Gopherus polyphemus), endemic to the southeastern part of United States, is a keystone species that provides ecological support over 350 species. Deforestation, urbanization, road mortality, and disease, past 100 years, have caused population decline by 80%, causing Florida declare threatened Little known about gut microbiome, which could play major role in health. This study aimed better understand health biology this through characterization analysis their microbiomes Abacoa Greenway Southeastern using next-generation sequencing survey microbiome. Major findings include: high levels alpha diversity; lack significant difference male female diversity taxonomic composition profiles and, counterintuitively, beta was less disparate than phyla associated with fiber fermentation short-chain fatty acid metabolism, Firmicutes Bacteroidetes, predominated all samples. Notable probiotic taxa present included: Lachnospiraceae Clostridium butyricum. Potentially pathogenic Helicobacter sp. Mollicutes Pathogenetic undetected: Helicobacter, Mycoplasma spp. These results add understudied reptiles

Language: Английский

Citations

0
Prebiotic potential of spent brewery grain – In vitro study DOI Creative Commons
Marcin Kruk, Piotr Lalowski, Magdalena Płecha

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 463, P. 141254 - 141254

Published: Sept. 13, 2024

Language: Английский

Citations

0
The role of gut microbiota in a generalist, golden snub-nosed monkey, adaptation to geographical diet change DOI Creative Commons
Yuhang Li, Yujie Yan,

Haojie Wu

et al.

Animal Microbiome, Journal Year: 2024, Volume and Issue: 6(1)

Published: Nov. 5, 2024

Changes in diet causing ecological stress pose a significant challenge to animal survival. In response, the gut microbiota, crucial part of host's digestive system, exhibits patterns change reflective alterations food component. The impact temporal dietary shifts on microbiota has been elucidated through multidimensional modeling both component and macronutrient intake. However, broad distribution wild generalist intricate complexity their hinder our capacity ascertain degree which assist adapting spatial variations. We examined variation microbial community according changes colobine monkey with regional variable diet, golden snub-nosed (Rhinopithecus roxellana). Specifically, we analyse interactions between component, intake community. compared monkeys from four populations by quantifying intake, sequencing 16S rRNA macro-genomes faecal samples 44 individuals. found differences diets compositions, nutrient space among some populations. Variations composition across distinct mirror disparities notable emphasis carbohydrate. Geographical will result variation, corresponding driving compositions abundances microbiota. Importantly, associated core functions does not vary, non-core fluctuating response This characteristic may enable species heavily reliant for digestion adapt changes. Our results further understanding roles play formation host niches.

Language: Английский

Citations

0
The Lachnospiraceae-butyric acid axis and its role in glucocorticoid-associated osteonecrosis DOI Creative Commons

Mingbin Guo,

Shuai He, Wei Song

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 11, 2024

Glucocorticoids (GCs) are key inducers of osteonecrosis, yet not all patients treated with GCs develop glucocorticoid-associated osteonecrosis (GAON). The factors mediating this relationship unclear. Studies have shown that gut microbiota and their metabolites influence bone metabolism, but role in GAON is This study aimed to explore the connection between microbiota. Through bidirectional Mendelian randomization analysis, we identified 14 microbial taxa, including Lachnospiraceae (IVW, P = 0.011), associated GAON. RNA-seq analysis revealed differentially expressed genes (DEGs) were enriched for intestinal inflammatory response mechanisms. We then compared who developed (17 cases), those did (GAnON, 15 untreated (Blank, cases) composition, short-chain fatty acids (SCFAs), serum factors. Our findings indicated a decrease abundance (GAON 17.13%, GAnON 12.51%, Blank 24.52%) GC-treated patients. Serum (IL-17 A, IL-33, TNF-α) (59.603 ± 12.147, 89.337 20.714, 42.584 9.185) showed significant differences (1.446 0.683, 11.534 4.705, 4.682 1.48) (25.353 8.181, 32.527 7.352, 12.49 3.217) groups, negative correlation these levels. Butyric acid levels SCFAs varied among groups (P<0.01) correlated Controlled experiments rats demonstrated butyric acid's osteoprotective development (P<0.01). In conclusion, our suggests reduced levels, along increased inflammation due use, contribute may mediate effects inflammation. Butyrate supplementation could potentially reduce incidence, offering novel approach its clinical management.

Language: Английский

Citations

0