Briefings in Bioinformatics,
Journal Year:
2024,
Volume and Issue:
26(1)
Published: Nov. 22, 2024
Vaccine
development
is
one
of
the
most
promising
fields,
and
multi-epitope
vaccine,
which
does
not
need
laborious
culture
processes,
an
attractive
alternative
to
classical
vaccines
with
advantage
safety,
efficiency.
The
rapid
algorithms
accumulation
immune
data
have
facilitated
advancement
computer-aided
vaccine
design.
Here
we
systemically
reviewed
in
silico
resource,
for
different
steps
computational
design,
including
immunogen
selection,
epitope
prediction,
construction,
optimization,
evaluation.
performance
available
tools
on
prediction
immunogenicity
evaluation
was
tested
compared
benchmark
datasets.
Finally,
discuss
future
research
direction
construction
a
multiepitope
vaccine.
Plants,
Journal Year:
2024,
Volume and Issue:
13(14), P. 1920 - 1920
Published: July 12, 2024
This
study
investigates
the
impact
of
methyl
jasmonate
(MeJA)
on
volatile
oil
composition
Schizonepeta
tenuifolia
and
elucidates
function
StTPS45
gene,
a
key
player
in
terpenoid
biosynthesis.
The
effect
different
concentrations
MeJA
(0,
50,
100,
200,
300
μmol/L)
growth
S.
adventitious
bud
clusters
was
analyzed
over
20
d
period.
Using
gas
chromatography–mass
spectrometry
(GC-MS),
17
compounds
were
identified
from
tenuifolia.
Significant
changes
levels
major
monoterpenes,
including
increased
contents
(+)-limonene
(+)-menthone,
observed,
particularly
at
higher
MeJA.
Analysis
transcriptome
data
three
groups
treated
with
0,
μmol/L
revealed
significant
gene
expression
profiles
following
treatment.
At
100
MeJA,
most
terpene
synthase
(TPS)
genes
overexpressed.
Additionally,
functional
predictions
suggested
that
acts
as
germacrene
D
synthase.
Therefore,
cloned
expressed
Escherichia
coli,
enzyme
activity
assays
confirmed
its
Molecular
docking
structural
prediction
further
specific
interactions
farnesyl
diphosphate
(FPP),
aligning
role
synthesis
pathway.
These
findings
provide
valuable
insights
into
modulation
secondary
metabolite
pathways
by
signaling
underscore
potential
genetic
engineering
approaches
to
enhance
production
terpenoids
medicinal
plants.
A
recent
study
by
Hanna
and
collaborators
assessed
the
presence
of
COVID-19
mRNA
productsin
placenta
umbilical
cord
blood
following
maternal
“vaccination”
during
human
pregnancy.Their
analysis
2
pregnant
women
revealed
that
genetic
injections
was
detected
inboth
placentas.
Spike
protein
expression
confirmed
in
one
them.
Furthermore,the
engineered
mother,
wherethose
samples
were
available
for
analysis.
They
also
found
integrity
injected
varied
across
samples,
but
overall,
a
substantial
or
even
overwhelming
majority
consisted
non-integrous
species.
The
authors
provide
several
theoretical
reasons
they
believe
explain
their
experiential
observations.
Based
on
results
shifting
aim,
indicate
benefits
suggest
related
gene
therapies,
particularly
mRNA-based
treatments,
may
have
great
promise
as
prenatal
therapy.
This
article
analyses
findings
interpretations
identifies
gaps
inconsistencies
explanations.
It
provides
short
synopsis
update
known
problems
with
outlines
major
pitfalls
unknowns
underlying
thepromised
intended
therapy
applications.
World Journal of Biology Pharmacy and Health Sciences,
Journal Year:
2024,
Volume and Issue:
20(2), P. 702 - 724
Published: Nov. 30, 2024
Messenger
RNA
(mRNA)
vaccines
have
quickly
become
a
potent
tool
in
cancer
immunotherapy,
with
the
potential
to
personalise
treatment
and
get
around
many
of
drawbacks
conventional
therapies.
With
an
emphasis
on
their
processes,
technological
developments,
clinical
applications,
this
study
examines
scientific
breakthroughs
difficulties
surrounding
mRNA
vaccines.
We
begin
by
investigating
ways
which
work
stimulate
immune
system,
encode
antigens
specific
tumours,
elicit
anti-tumor
response.
also
look
at
variety
are
currently
being
deployed
emphasize
successes
studies,
especially
when
combined
checkpoint
inhibitors.
The
effectiveness
safety
these
been
significantly
increased
such
as
enhancements
stability,
design,
delivery
systems
(such
lipid
nanoparticles
polyplexes).
Emerging
technologies
like
circular
self-amplifying
present
promising
opportunities
for
enduring
more
But
there
still
limitations
vaccines,
immunogenicity,
degradation,
effective
vivo
delivery.
Concerns
about
possible
adverse
effects
long-term
applications
covered
review.
Despite
obstacles,
novel
approaches
developed
boost
antigen
expression,
optimize
systems,
improve
establishing
crucial
component
tailored
immunotherapy.
These
developments
provide
foundation
upcoming
advances
vaccine
technology
represent
major
breakthrough
fight
against
cancer.
Briefings in Bioinformatics,
Journal Year:
2024,
Volume and Issue:
26(1)
Published: Nov. 22, 2024
Vaccine
development
is
one
of
the
most
promising
fields,
and
multi-epitope
vaccine,
which
does
not
need
laborious
culture
processes,
an
attractive
alternative
to
classical
vaccines
with
advantage
safety,
efficiency.
The
rapid
algorithms
accumulation
immune
data
have
facilitated
advancement
computer-aided
vaccine
design.
Here
we
systemically
reviewed
in
silico
resource,
for
different
steps
computational
design,
including
immunogen
selection,
epitope
prediction,
construction,
optimization,
evaluation.
performance
available
tools
on
prediction
immunogenicity
evaluation
was
tested
compared
benchmark
datasets.
Finally,
discuss
future
research
direction
construction
a
multiepitope
vaccine.
