ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(51), P. 70284 - 70296
Published: Dec. 12, 2024
Skin
infections
are
currently
a
worldwide
emergency
as
antibiotic-resistant
bacteria
spreading,
leading
to
the
ineffectiveness
of
most
antibiotics
and
antibacterial
strategies.
Consequently,
there
is
an
urgency
developing
testing
innovative
therapies.
As
traditional
2D
cell
culture
planktonic
can
be
obsolete
due
their
incapability
resembling
complex
infection
environment,
3D
in
vitro
skin
models
powerful
tool
test
validate
In
this
article,
epidermis–dermis
model
has
been
developed
biofabricated
broadly
available,
reaching
balance
between
simplicity
reproducibility
its
complexity
terms
wound,
infection,
treatment
response.
The
results
really
promising,
comprehensive
physical
barrier.
To
further
investigate
model,
controlled
wounding,
antibiotic
treatments
were
performed.
remarkable:
Not
only
was
unwounded
epidermal
barrier
able
partially
stop
bacterial
proliferation,
but
entire
system
reacted
both
wound
complete
way.
Extracellular
matrix
deposition
remodeling,
inflammatory
response,
antimicrobial
peptide
production,
change
cellular
behaviors,
from
epithelial
mesenchymal
fibroblasts
myofibroblasts,
witnessed,
with
different
extents
depending
on
strain.
addition,
response
administration
opposite
for
two
infections,
probably
revealing
release
endotoxins
during
Escherichia
coli
death.
conclusion,
presented
all
characteristics
future
landmark
platform
strategy
therapy
testing.
Polymers,
Journal Year:
2024,
Volume and Issue:
16(18), P. 2560 - 2560
Published: Sept. 10, 2024
Hydrogels
as
biomaterials
possess
appropriate
physicochemical
and
mechanical
properties
that
enable
the
formation
of
a
three-dimensional,
stable
structure
used
in
tissue
engineering
3D
printing.
The
integrity
hydrogel
composition
is
due
to
presence
covalent
or
noncovalent
cross-linking
bonds.
Using
various
methods
agents
crucial
for
adjusting
specific
biomedical
applications,
e.g.,
direct
bioprinting.
research
subject
was
mixtures
gel-forming
polymers:
sodium
alginate
gelatin.
polymers
were
cross-linked
ionically
with
addition
CaCl2
solutions
concentrations
(10%,
5%,
2.5%,
1%)
covalently
using
squaric
acid
(SQ)
dialdehyde
starch
(DAS).
Initially,
polymer
mixture’s
procedure
determined.
obtained
materials
characterized
by
property
tests,
swelling
degree,
FTIR,
SEM,
thermal
analysis,
biological
research.
It
found
tensile
strength
hydrogels
1%
2.5%
higher
than
after
10%
solution
(130
kPa
80
kPa,
respectively),
at
same
time,
elongation
break
increased
(to
75%),
stiffness
decreased
(Young
Modulus
169
104
respectively).
Moreover,
lowering
concentration
from
reduced
final
material’s
toxicity.
showed
lower
degradation
temperatures
weight
losses
those
therefore
less
thermally
stable.
Additional
SQ
DAS
had
only
minor
effect
on
hydrogels,
but
especially
use
elasticity.
All
tested
porous
structure,
pores
irregular
shape
heterogenic
size,
their
degree
initially
sharply
value
approx.
1000%
during
first
6
h,
finally,
it
stabilized
level
1200–1600%
24
h.
viscosity
6%
gelatin
2%
without
similar,
they
slightly
shear-thinning.
concluded
mixture
containing
presented
optimal
gel
improved
hydrogel’s
biocompatibility
positively
influenced
efficiency.
chemical
additionally
printability.
In
conclusion,
among
systems,
gelatin–2%
applications
potentially
suitable
Molecules,
Journal Year:
2025,
Volume and Issue:
30(3), P. 649 - 649
Published: Feb. 1, 2025
Three-dimensional
bioprinting
allows
for
the
fabrication
of
structures
mimicking
tissue
architecture.
This
study
aimed
to
develop
a
gelatin-based
bioink
bioprinted
simplified
skin
model.
The
printability
and
chemical-physical
properties
were
evaluated
by
varying
concentrations
gelatin
(10,
15,
20%)
in
semi-crosslinked
alginate-based
calcium
chloride
(100,
150,
200
mM)
post-printing
crosslinking.
For
increasing
concentration,
formulations
have
shear
thinning
behavior
with
viscosity,
filament
bending
angle
increases,
spreading
ratio
value
approaches
1,
shape
fidelity
printing
resolution
improve.
However,
formulation
containing
20%
was
not
homogeneous,
resulting
also
poor
properties.
morphology
pores,
degradation,
swelling
depend
on
CaCl2
concentrations,
but
significant
way.
samples
15%
crosslinked
150
mM
been
selected
bilayer
model
human
fibroblasts
keratinocytes.
showed
homogeneous
distribution
viable
proliferating
cells
over
up
14
days
vitro
culture.
allowed
3D
model,
potential
applications
bioactivity
pro-reparative
molecules
drug
evaluation.
Journal of Bioactive and Compatible Polymers,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 18, 2025
Liver
transplantation
is
the
only
treatment
for
patients
with
end-stage
liver
diseases
yet
limited
availability
of
donor
organs
greatly
restricts
this
option.
One
innovative
approach
to
mitigate
challenge
involves
biofabrication
tissues
through
3D
Bioprinting
technology.
Gelatin
Methacrylamide
(GelMA),
a
modified
form
gelatin
one
extensively
studied
hydrogel
bioprinting
soft
tissues.
This
study
reports
non-cytotoxic,
printable
bioink
formulation
composed
10%
GelMA,
3%
and
0.25%
LAP
(GelMA-G-L).
We
have
in
vivo
integration
printed
construct
within
rat
tissue
following
upto
30-days
implantation
period.
Histological
examination
revealed
that
GelMA-G-L
had
successfully
integrated
surrounding
tissue,
displaying
adequate
vascularization
no
indications
adverse
immune
responses
or
rejection
signs.
Bioengineering,
Journal Year:
2025,
Volume and Issue:
12(3), P. 230 - 230
Published: Feb. 24, 2025
In
recent
times,
with
the
need
for
a
reduction,
refinement,
and
replacement
of
in
vivo
animal
testing,
there
has
been
an
increasing
demand
use
relevant
vitro
human
cell
systems
drug
development.
There
is
also
great
skin
tissue
various
wounds
burns.
Furthermore,
cell-based
can
be
used
to
investigate
side
effects
(toxicity
irritation)
penetration
topical
preparations.
this
study,
exploratory
experiments
were
performed
produce
artificial
epidermis
using
two
hydrogel
scaffolds,
alginate
GelMA
C.
The
amount
keratinocytes
added
matrix
(10–50–100
×
106/mL)
duration
maturation
(fresh,
1–3–4
weeks)
optimized
extensive
study.
behavior
structure
hydrogels
functionally
morphologically
assessed.
permeability
order
caffeine
tested
barriers
was
following:
>
C
cellulose
acetate
membrane
rat
skin.
It
concluded
that
provides
more
favorable
environment
survival
differentiation
(as
demonstrated
by
histology
immunohistochemistry)
than
alginate.
3-week
incubation
50
106/mL
number
proved
most
beneficial
given
system.
This
study
data
first
time
on
multifactorial
optimization
potential
substitutes
manufacturing.
these
results
engineering,
fabricated
preparations
must
biocompatibility
from
physical
mechanical
point
views.
Materials Today Bio,
Journal Year:
2024,
Volume and Issue:
28, P. 101210 - 101210
Published: Aug. 28, 2024
Skin
aging
is
the
phenomenon
of
degenerative
changes
in
structure
and
function
skin
tissues
over
time
manifested
by
a
gradual
loss
elasticity
firmness,
an
increased
number
wrinkles,
hyperpigmentation.
anti-aging
refers
to
reduction
through
medical
cosmetic
technologies.
In
recent
years,
new
biomaterials
have
been
continuously
developed
for
improving
appearance
mechanical
tissue
filling,
regulating
collagen
synthesis
degradation,
inhibiting
pigmentation,
repairing
barrier.
This
review
summarizes
mechanisms
associated
with
aging,
describes
that
are
commonly
used
aesthetics
their
possible
modes
action,
discusses
application
strategies
this
area.
Moreover,
synergistic
effects
such
other
active
ingredients,
as
stem
cells,
exosomes,
growth
factors,
antioxidants,
on
regeneration
evaluated.
Finally,
challenges
development
prospects
field
discussed,
novel
ideas
future
innovations
area
summarized.
Materials Research Express,
Journal Year:
2024,
Volume and Issue:
11(7), P. 075307 - 075307
Published: July 1, 2024
Abstract
GelMA
hydrogels
are
prominent
in
biomedical
applications
due
to
their
innate
extracellular
matrix
mimicking
properties.
They
exhibit
favorable
properties
for
cell
proliferation
and
formation
of
light-induced
hydrophilic
cross-linked
structures.
However,
there
is
limited
research
on
the
effect
variations
starting
material
(gelatin)
physical,
mechanical
biological
In
this
study,
Gelatin
Methacrylic
Anhydride
(GelMA)
were
synthesized
from
two
different
products
type
B
gelatin
loaded
with
polyvinylpyrrolidone
(PVP)
nanoparticles
by
electrospray
method.
Chemical
structural
analyses
performed
FTIR,
1
HNMR,
TNBS
SEM,
respectively.
Mechanical
evaluated
compression
tests.
Cytocompatibility
was
XTT
analysis.
obtained
brands
have
suitable
pore
size,
strength,
swelling
cytocompatibility,
making
them
various
applications.
addition,
addition
PVP
can
make
useful
drug
delivery
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Sept. 24, 2024
In
order
to
recreate
the
complexity
of
human
organs,
field
tissue
engineering
and
regenerative
medicine
has
been
focusing
on
methods
build
organs
from
bottom
up
by
assembling
distinct
small
functional
units
consisting
a
biomaterial
cells.
This
bottom-up
requires
bioinks
that
can
be
assembled
3D
bioprinting
permit
fast
vascularization
construct
ensure
survival
embedded
To
this
end,
molecular
weight
alginate
(SMWA)
gel
porogen
is
presented
herein.
Alginate
biocompatible
biomaterial,
which
easily
converted
into
gels
with
procedure
reported
in
article.
The
SMWA
mixed
photo-crosslinkable
hydrogels
leached
hydrogel
post-crosslinking
increase
porosity
facilitate
vascularization.
As
proof
concept,
system
tested
commonly
used
Gelatin
Methacryloyl
(GelMA).
porogen-GelMA
blend
proven
bioprintable.
Incubating
for
20
min
low
concentration
phosphate
buffered
saline
sodium
citrate
solution
significantly
reduces
remaining
.
intent
completely
leach
was
abandoned,
as
longer
incubation
times
higher
concentrations
were
detrimental
endothelial
proliferation.
Nonetheless,
even
remnants
left
hydrogel,
created
improves
viability,
growth
factor
signaling,
vasculogenesis,
angiogenesis
bioprinted
structures.
article
concludes
usage
improve
assembly
microvasculature
technology
benefit
large
scaffolds
high
cell
density
through
improving
viability
allowing
faster
