Enhanced Piezoelectric Performance of PVDF-TrFE Nanofibers through Annealing for Tissue Engineering Applications

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract This study investigates bioelectric stimulation’s role in tissue regeneration by enhancing the piezoelectric properties of tissue-engineered grafts using annealed poly(vinylidene fluoride-trifluoroethylene) (PVDF-TrFE) scaffolds. Annealing at temperatures 80°C, 100°C, 120°C, and 140°C was assessed for its impact on material physiological utility. Analytical techniques such as Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD) revealed increased crystallinity with higher annealing temperatures, peaking β-phase content 140°C. Electron Microscopy (SEM) showed that scaffolds had enhanced lamellar structures, porosity, maximum response. Mechanical tests indicated improved elastic modulus, tensile strength, substrate stiffness, aligning these soft tissues. In vitro assessments Schwann cells demonstrated favorable responses, cell proliferation, contraction, extracellular matrix attachment. Additionally, genes linked to production, vascularization, calcium signaling were upregulated. The foreign body response C57BL/6 mice, evaluated through Hematoxylin Eosin (H&E) Picrosirius Red staining, no differences between scaffold groups, supporting potential future functional evaluation group repair.

Language: Английский

Decellularized Green and Brown Macroalgae as Cellulose Matrices for Tissue Engineering

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(12), P. 390 - 390

Published: Dec. 23, 2024

Scaffolds resembling the extracellular matrix (ECM) provide structural support for cells in engineering of tissue constructs. Various material sources and fabrication techniques have been employed scaffold production. Cellulose-based matrices are interest due to their abundant supply, hydrophilicity, mechanical strength, biological inertness. Terrestrial marine plants offer diverse morphologies that can replicate ECM various tissues be isolated through decellularization protocols. In this study, three macroalgae species—namely Durvillaea poha, Ulva lactuca, Ecklonia radiata—were selected morphological variation. Low-intensity, chemical treatments were developed each species maintain native cellulose structures within while facilitating clearance DNA pigment. generated from seaweed non-toxic human dermal fibroblasts but only fibrous inner layer those derived E. radiata supported cell attachment maturation over seven days culture. These findings demonstrate potential radiata-derived scaffolds skin highlight influence on efficiency, properties, utility.

Language: Английский