An Agrobacterium‐mediated base editing approach generates transgene‐free edited banana

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Genome editing for the development of improved varieties is supported by possibility segregating out editor T-DNA cassette after genome in many crop species. Removal prevents potential continuous activity transformed plant and furthermore facilitates regulatory approval. While transgene outcrossing exogenous sequences possible crops, this not case vegetatively propagated sterile such as Cavendish bananas. Therefore, gene techniques leading to transgene-free edited plants are essential untap those crops. Here, we present a method banana (Musa spp.) through co-editing strategy. A novel Agrobacterium tumefaciens-mediated approach combining embryogenesis chlorsulfuron selection was established validated whole sequencing. Editing acetolactate synthase (MaALS) genes using base allows effective plants. Moreover, plantlets were regenerated with mutations at two target sites, indicating that strategy can be used multiple genomic sites. The presented efficient represents first report

Language: Английский

Crash‐and‐dash: a new era in tree genome editing

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский