Synergistic dual Ag/Cu ion implantation to enhance antimicrobial defense on Boston Keratoprosthesis DOI Creative Commons
Silvia González Gómez, Gerard Boix‐Lemonche, Jordi Orrit‐Prat

et al.

Biomaterials Research, Journal Year: 2025, Volume and Issue: 29

Published: Jan. 1, 2025

The Boston keratoprosthesis (BKPro) is a critical device for vision restoration in complex cases of corneal blindness, although its long-term retention challenged by infection risks. This study aims to enhance the antimicrobial properties titanium (Ti) backplate BKPro ion implanting silver and copper ions achieve effective control while maintaining cytocompatibility. Research on modifications limited, metallic like Ag Cu show promise biomaterial improvement, their effects human keratocytes (HCKs) require further study. were implanted onto rough Ti surfaces, as mono- coimplantations. Cytotoxicity was assessed HCKs, efficacy tested against Pseudomonas aeruginosa Candida albicans . After 21 d, monoimplanted samples released 300.4 ppb + , coimplanted 427.5 272.3 ions, 567.0 ions. All ion-implanted surfaces supported HCK proliferation, exhibited no cytotoxicity, showed strong activity. Ag-implanted provided antibacterial through membrane disruption reactive oxygen species generation, Cu-implanted antifungal via impaired enzymatic functions species. Coimplanted AgCu demonstrated synergistic effects, resulting from synergy between bactericidal actions oxidative stress contributions Cu. Additionally, enhanced adhesion under co-culture conditions. In conclusion, implantation effectively imparts BKPro, reducing risks preserving compatibility with cells.

Language: Английский

Synergistic dual Ag/Cu ion implantation to enhance antimicrobial defense on Boston Keratoprosthesis DOI Creative Commons
Silvia González Gómez, Gerard Boix‐Lemonche, Jordi Orrit‐Prat

et al.

Biomaterials Research, Journal Year: 2025, Volume and Issue: 29

Published: Jan. 1, 2025

The Boston keratoprosthesis (BKPro) is a critical device for vision restoration in complex cases of corneal blindness, although its long-term retention challenged by infection risks. This study aims to enhance the antimicrobial properties titanium (Ti) backplate BKPro ion implanting silver and copper ions achieve effective control while maintaining cytocompatibility. Research on modifications limited, metallic like Ag Cu show promise biomaterial improvement, their effects human keratocytes (HCKs) require further study. were implanted onto rough Ti surfaces, as mono- coimplantations. Cytotoxicity was assessed HCKs, efficacy tested against Pseudomonas aeruginosa Candida albicans . After 21 d, monoimplanted samples released 300.4 ppb + , coimplanted 427.5 272.3 ions, 567.0 ions. All ion-implanted surfaces supported HCK proliferation, exhibited no cytotoxicity, showed strong activity. Ag-implanted provided antibacterial through membrane disruption reactive oxygen species generation, Cu-implanted antifungal via impaired enzymatic functions species. Coimplanted AgCu demonstrated synergistic effects, resulting from synergy between bactericidal actions oxidative stress contributions Cu. Additionally, enhanced adhesion under co-culture conditions. In conclusion, implantation effectively imparts BKPro, reducing risks preserving compatibility with cells.

Language: Английский

Citations

0