Exposing the cellular situation: findings from single cell RNA sequencing in breast cancer DOI Creative Commons
Gaofeng Ni,

Xinhan Li,

Wenyang Nie

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 6, 2025

Breast Cancer (BC) ranks among the top three most prevalent cancers globally and stands as principal contributor to cancer-related fatalities women. In spite of substantial occurrence rate BC, early stage this disease is generally regarded curable. However, intra-tumor heterogeneity presents a formidable obstacle success effective treatment. research, single cell RNA sequencing was utilized dissect tumor microenvironment within BC. Slingshot, CytoTRACE Monocle 2 were applied illustrate differentiation process each subpopulation in pseudotime sequence. To comprehensively comprehend cells (TCs) an analysis upstream transcription factors carried out via pySCENIC, while downstream pathway enrichment conducted through KEGG, GO GSEA. The prognosis model established based on bulk data obtained from TCGA GEO databases. Knock-down experiments also implemented explore function factor CEBPD TCs. Our in-depth identified eight types. Notably, TCs predominantly found epithelial cells. classification further uncovered five unique subpopulations, with one characterized by high UGDH expression. This shown possess distinct metabolic features metabolism-related investigations. intricate communication modalities different types effectively demonstrated means CellChat. Additionally, crucial factor, CEBPD, identified, which pronounced propensity towards tumors harbored potential tumor-advancing characteristics. Its role promoting cancer subsequently verified vitro knock-down experiments. Moreover, prognostic developed, risk score genes incorporated model. Through comparing prognoses UTRS levels, it determined that group had less favorable prognosis. These outcomes contributed elucidation complex interrelationships BC microenvironment. By specifically targeting certain subpopulations TCs, novel treatment strategies could potentially be devised. study shed light direction future research should take, furnishing valuable information can enhance regimens.

Language: Английский

Exposing the cellular situation: findings from single cell RNA sequencing in breast cancer DOI Creative Commons
Gaofeng Ni,

Xinhan Li,

Wenyang Nie

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 6, 2025

Breast Cancer (BC) ranks among the top three most prevalent cancers globally and stands as principal contributor to cancer-related fatalities women. In spite of substantial occurrence rate BC, early stage this disease is generally regarded curable. However, intra-tumor heterogeneity presents a formidable obstacle success effective treatment. research, single cell RNA sequencing was utilized dissect tumor microenvironment within BC. Slingshot, CytoTRACE Monocle 2 were applied illustrate differentiation process each subpopulation in pseudotime sequence. To comprehensively comprehend cells (TCs) an analysis upstream transcription factors carried out via pySCENIC, while downstream pathway enrichment conducted through KEGG, GO GSEA. The prognosis model established based on bulk data obtained from TCGA GEO databases. Knock-down experiments also implemented explore function factor CEBPD TCs. Our in-depth identified eight types. Notably, TCs predominantly found epithelial cells. classification further uncovered five unique subpopulations, with one characterized by high UGDH expression. This shown possess distinct metabolic features metabolism-related investigations. intricate communication modalities different types effectively demonstrated means CellChat. Additionally, crucial factor, CEBPD, identified, which pronounced propensity towards tumors harbored potential tumor-advancing characteristics. Its role promoting cancer subsequently verified vitro knock-down experiments. Moreover, prognostic developed, risk score genes incorporated model. Through comparing prognoses UTRS levels, it determined that group had less favorable prognosis. These outcomes contributed elucidation complex interrelationships BC microenvironment. By specifically targeting certain subpopulations TCs, novel treatment strategies could potentially be devised. study shed light direction future research should take, furnishing valuable information can enhance regimens.

Language: Английский

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