Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease,
Journal Year:
2025,
Volume and Issue:
1871(5), P. 167787 - 167787
Published: March 9, 2025
Retinopathy
of
prematurity
(ROP),
a
leading
cause
blindness
in
premature
infants,
is
characterized
by
retinal
vaso-obliteration
during
hyperoxia
and
pathological
neovascularization
(NV)
relative
hypoxia
phase.
Current
treatments,
which
focus
on
the
late
stages
neovascularization,
are
associated
with
numerous
side
effects.
Studies
demonstrated
that
discoidin
domain
receptor
2
(DDR2),
collagen-binding
tyrosine
kinase,
inhibits
experimental
choroidal
participates
tumor
angiogenesis.
However,
role
DDR2
ROP
underlying
mechanisms
unclear.
In
this
study,
we
initially
found
expressed
mouse
physiological
vascular
development
significantly
decreased
phase
followed
increase
oxygen-induced
retinopathy
(OIR)
model.
Early
upregulation
before
attenuates
vaso-obliteration,
reduces
promotes
maturation.
Additionally,
increased
number
microglia
around
blood
vessels
induced
anti-inflammatory
M2
polarization.
Furthermore,
STAT6/TGF-β
signaling
pathway
suppressed
was
activated
after
upregulation.
conclusion,
attenuated
inhibited
switching
polarization
from
M1
to
phenotype
via
OIR.
This
suggests
could
be
novel
target
for
early
treatment
ROP.
Journal of Clinical Medicine,
Journal Year:
2025,
Volume and Issue:
14(1), P. 245 - 245
Published: Jan. 3, 2025
Background/Objectives:
Diabetes
is
a
well-recognised
factor
inducing
plethora
of
corneal
alterations
ranging
from
dry
eye
to
reduced
sensibility,
epithelial
defects,
and
cicatrisation.
This
cohort
study
aimed
assess
the
efficacy
novel
ophthalmic
solution
combining
cross-linked
hyaluronic
acid
(CHA),
chondroitin
sulfate
(CS),
inositol
(INS)
in
managing
diabetes-induced
alterations.
Specifically,
it
evaluated
solution’s
impact
on
tear
breakup
time
(TBUT),
ocular
surface
disease
index
(OSDI),
sensitivity
after
three
months
treatment.
Additionally,
potential
promote
wound
healing
was
examined.
Methods:
Two
different
populations
were
retrieved
database;
first
one
composed
20
diabetic
subjects
treated
for
with
CAH-CS
(OPHTAGON
srl,
Rome,
Italy),
while
second
group
who
did
not
want
use
any
lubricant
or
other
The
outcome
measures
TBUT,
OSDI
score,
measured
using
Cochet–Bonnet
aesthesiometer.
To
investigate
wound-healing
properties,
vitro
tests
conducted
two
cell
lines,
comparing
results
scratch
without
solution.
Results:
indicate
that
CHA-CS
significantly
improved
film
stability,
as
evidenced
by
an
increased
TBUT
reduction
symptoms
reflected
lower
scores.
Moreover,
associated
enhanced
patients.
In
assays,
promoted
motility,
suggesting
supportive
role
tissue
repair
compared
untreated
cells.
Conclusions:
Collectively,
suggest
could
serve
innovative
tool
treatment
patients
delayed
sensitivity.
Clinical
trial
registration
number:
Trial.gov
NCT06573606.
Journal of Cellular Physiology,
Journal Year:
2025,
Volume and Issue:
240(1)
Published: Jan. 1, 2025
ABSTRACT
In
addition
to
proteins
such
as
collagen
(Col)
and
fibronectin,
the
extracellular
matrix
(ECM)
is
enriched
with
bulky
proteoglycan
molecules
hyaluronic
acid
(HA).
However,
how
ECM
proteoglycans
collectively
regulate
cellular
processes
has
not
been
adequately
explored.
Here,
we
address
this
question
by
studying
cytoskeletal
focal
adhesion
organization
dynamics
on
cells
cultured
polyacrylamide
hydrogels
functionalized
Col,
HA
a
combination
of
Col
(Col/HA).
We
show
that
fastest
migration
substrates
attributed
presence
smaller
weaker
adhesions.
Integrin
1
co‐localization
its
association
CD44—which
receptor
for
HA,
insensitivity
cell
spreading
RGD
suggests
adhesions
are
formed
via
integrin
bound
CD44.
Consistent
this,
formation
motility
were
inhibited
when
CD44
was
knocked
out.
Collectively,
our
results
suggest
drives
fast
substrates.
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(2), P. 178 - 178
Published: Jan. 25, 2025
Keratan
sulfate
(KS)
is
a
negatively
charged
carbohydrate
linked
to
proteins.
Several
KS-bearing
structural
glycosaminoglycans
participate
maintain
the
homeostasis
of
functional
extracellular
matrix.
Dysfunction
its
biochemical
composition
and
structure
might
therefore
lead
pathological
situations.
For
this
reason,
imaging
KS
in
tissues
an
important
diagnostic
tool.
Here,
we
describe
identification
paratope
derived
from
ancestral
anti-KS
IgG
mAb
MZ15,
as
well
engineering,
recombinant
expression
E.
coli,
purification
single-chain
variable
fragment
(ScFv).
The
ScFv
enabled
vitro
cryosections
rat
cornea
by
immunofluorescence
microscopy
comparable
MZ15.
Bioengineering,
Journal Year:
2025,
Volume and Issue:
12(3), P. 259 - 259
Published: March 5, 2025
During
corneal
wound
healing,
transforming
growth
factor-beta
1
(TGF-β1)
causes
differentiation
of
quiescent
keratocytes
into
myofibroblasts.
Decorin
has
been
investigated
as
a
promising
anti-fibrotic
therapeutic
for
healing
due
to
its
interaction
with
TGF-β1,
collagen,
and
cell
surface
receptors.
In
this
study,
novel
microfluidic
method
coating
aligned
collagen
fibrils
decorin
was
developed
mimic
the
presence
within
stroma.
found
bind
selectively
remained
bound
at
least
five
days.
To
investigate
effects
coatings
on
keratocyte
activation,
primary
rabbit
were
cultured
in
TGF-β1
5
days
substrates
or
without
stained
α-smooth
muscle
actin
(α-SMA).
The
expression
α-SMA
reduced
by
similar
amounts
monomeric
(40%),
random
(32%),
(32%)
coated
controls.
However,
differentially
expressed
between
not
decorin,
significantly
lower
uncoated
compared
monomers.
Addition
directly
culture
media,
had
limited
effect
reducing
myofibroblast
differentiation.
Taken
together,
these
results
demonstrate
importance
topography
ECM
composition
activation.
Fishes,
Journal Year:
2025,
Volume and Issue:
10(3), P. 130 - 130
Published: March 17, 2025
Popular
foods
such
as
sushi
and
sashimi
depend
on
the
quality
of
raw
fish
meat
to
maintain
consumer
satisfaction.
Recently,
dietary
insect
meal
insect-derived
substances
have
been
extensively
studied
for
application
in
aquaculture
a
protein
alternative
or
immunostimulant.
However,
impact
functional
teleosts
remains
unclear.
Here,
we
investigated
influence
inclusion
silkrose-BM,
novel
bioactive
polysaccharide
derived
from
silkworm,
Bombyx
mori,
yellowtail
(Seriola
quinqueradiata).
This
study
was
conducted
by
comparing
two
groups
given
different
feeds,
commercial
EP
feeds
containing
Silkrose-BM
(0.1%),
after
culture
period
six
months
separate
floating-net
cages.
The
were
specifically
cut
into
loins
several
parameters
observed,
including
proximate,
color
changes,
total
collagen,
drip
loss,
muscle
histology,
gene
expression
(qRT-PCR).
results
color-change
analysis
showed
that
discoloration
red
feed
group
occurred
faster
than
silkrose-BM
group,
indicating
an
antioxidant
property
silkrose-BM.
Dietary
also
significantly
reduced
loss
increased
collagen
content
meat.
Furthermore,
qRT-PCR
genes
related
lipid
degradation
downregulated
muscles
fed
In
contrast,
proximate
indicated
no
significant
change
nutritional
composition
between
groups.
Taken
together,
our
suggest
could
improve
minimizing
denaturation
inhibiting
oxidation
during
storage.
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
13
Published: March 27, 2025
The
glycocalyx
is
a
dynamic
carbohydrate-enriched
structure
that
forms
dense
coating
on
the
surface
of
animal
cells,
including
those
at
ocular
surface.
It
plays
critical
role
in
maintaining
cellular
functions
and
it
has
significant
influence
disease
processes.
At
surface,
glycoproteins
such
as
mucins
are
essential
for
hydration,
lubrication,
barrier
protection.
Proteoglycans
glycolipids
contribute
to
cell
signaling,
mediate
interactions
with
pathogens.
Alterations
implicated
spectrum
conditions,
dry
eye
disease,
allergies,
infections,
systemic
diseases
Graft-versus-host
(GVHD)
diabetes
mellitus.
Research
revealed
alterations
mucin
expression
aberrant
glycosylation
but
many
aspects
how
these
processes
remain
poorly
understood.
Advancing
our
knowledge
composition
function
offers
valuable
insights
into
pathophysiology
presents
opportunities
novel
glycocalyx-targeted
therapeutic
strategies
mitigate
progression
enhance
patient
care.
This
review
explores
current
glycocalyx,
emphasizing
its
implications
disease.
