Gastric Cancer Pre-Stage Detection and Early Diagnosis of Gastritis Using Serum Protein Signatures DOI Creative Commons
Shahid Aziz, Faisal Rasheed, Rabaab Zahra

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(9), P. 2857 - 2857

Published: April 30, 2022

A gastric cancer (GC) diagnosis relies on histopathology. Endoscopy rates are increasing. Helicobacter pylori infection is a major GC risk factor. In an effort to elucidate abundant blood biomarkers, and potentially reduce the number of diagnostic surgical interventions, we investigated sera biopsies from cohort 219 H. positive negative patients diagnosed with GC, gastritis, ulcers. This allowed comparative investigation different gastroduodenal diseases, exclusion protein changes resulting bacterial or inflammation mucosa when searching for GC-dependent proteins.High-definition mass spectrometry-based expression analysis tryptically digested proteins was performed, followed by multivariate statistical network analyses disease groups, respect status. Significantly regulated differing more than two-fold between groups were shortlisted, their role in gastritis discussed.We present data suffering mild advanced ulcers, early conjunction wealth metadata, clinical information, histopathological evaluation, We used samples pre-malignant stages extract prospective serum markers early-stage 29-protein marker panel containing, amongst others, integrin β-6 glutathione peroxidase. Furthermore, ten specific independent infection, provided. They include CRP, S100A9, kallistatin. The majority these previously discussed context GC. addition, detected hypoalbuminemia increased fibrinogen levels gastritis.Two panels suggested development multiplex tests diagnostics. For most elements contained panels, individual commercial available. Thus, envision design multi-protein assays, incorporating several all members, order gain level specificity that cannot be achieved testing single alone. As validation will take time, based albumin ratio may quick way forward. Its determination at primary/secondary care could significantly referrals endoscopy. Preventive measures high demand. presented this work contribute improved diagnostics, once they have been transferred research result practical tool.

Language: Английский

Immune evasion by cancer stem cells DOI Creative Commons
Hiroyuki Tsuchiya, Goshi Shiota

Regenerative Therapy, Journal Year: 2021, Volume and Issue: 17, P. 20 - 33

Published: March 12, 2021

Tumor immunity represents a new avenue for cancer therapy. Immune checkpoint inhibitors have successfully improved outcomes in several tumor types. In addition, currently, immune cell-based therapy is also attracting significant attention. However, the clinical efficacy of these treatments requires further improvement. The mechanisms through which cells escape response must be identified and clarified. Cancer stem (CSCs) play central role multiple aspects malignant tumors. CSCs can initiate tumors partially immunocompromised mice, whereas non-CSCs fail to form tumors, suggesting that initiation definitive function CSCs. fact more highly mice suggests evasion property may fundamental feature rather than tumor-initiating property. this review, we summarize studies elucidated how evade create an immunosuppressive milieu with focus on CSC-specific characteristics functions. These profound provide important clues development novel immunotherapies.

Language: Английский

Citations

99
Combinatorial therapy in tumor microenvironment: Where do we stand? DOI
Smita Kumari, Dia Advani, Sudhanshu Sharma

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2021, Volume and Issue: 1876(2), P. 188585 - 188585

Published: July 2, 2021

Language: Английский

Citations

83
Interplay Between Extracellular Matrix Remodeling and Angiogenesis in Tumor Ecosystem DOI Open Access
Rohit Siddhartha, Minal Garg

Molecular Cancer Therapeutics, Journal Year: 2023, Volume and Issue: 22(3), P. 291 - 305

Published: March 2, 2023

Studying the complex mechanisms of tumorigenesis and examining interactions neoplastic cells within tumor ecosystem are critical to explore possibility effective cancer treatment modalities. Dynamic is constantly evolving composed cells, extracellular matrix (ECM), secreted factors, stromal cancer-associated fibroblasts (CAF), pericytes, endothelial (EC), adipocytes, immune cells. ECM remodeling by synthesis, contraction, and/or proteolytic degradation components release matrix-sequestered growth factors create a microenvironment that promotes EC proliferation, migration, angiogenesis. Stromal CAFs multiple angiogenic cues (angiogenic cytokines, enzymes) which interact with proteins, thus contribute enhance proangiogenic/promigratory properties support aggressive growth. Targeting angiogenesis brings about vascular changes including reduced adherence junction basement membrane pericyte coverage, increased leakiness. This facilitates remodeling, metastatic colonization chemoresistance. Owing significant role denser stiffer in inducing chemoresistance, direct or indirect targeting being reported as major axis anticancer treatment. Exploring agents context specific manner may lead burden promoting conventional therapeutic effectiveness overcoming hurdles therapy resistance.

Language: Английский

Citations

25
Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis DOI Creative Commons
Mohamed E. Mahmoud, Amr Ahmed WalyEldeen,

Mohamed Abdel samie

et al.

The Journal of Basic and Applied Zoology, Journal Year: 2025, Volume and Issue: 86(1)

Published: Jan. 4, 2025

Abstract Background Endometriosis is a benign gynecological disease characterized by the growth of endometrial cells beyond uterus, forming endometriotic cyst tissues called ovarian endometriomas. MicroRNAs (miRNAs) are small, non-coding RNAs that epigenetically control physiological and pathological processes different diseases, including endometriosis. In this study, we screened expression levels 11-selected miRNAs, namely miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, miR-200b-3p, miR-16-5p, miR-222-3p in endometriomas relative to eutopic using quantitative real-time PCR (qPCR). addition, level mRNA lumican ( LUM ), an extracellular matrix proteoglycan (PG), putative target miR-21-5p was quantified qPCR. Results Our screening qPCR results showed 9 miRNAs were upregulated (miR-21-5p, miR-200b-3p), whereas 2 downregulated (miR-16-5p miR-222-3p) compared endometrium. A significant overexpression endometrioma further independently verified Using bioinformatics tools, Gene Ontology Kyoto Encyclopedia Genes Genomes pathways, protein–protein interactions, identified differentially expressed genes several pathways regulated may contribute endometriosis progression. Among them, found be significantly diminished Conclusion conclusion, study as potential factors endometriomas’ pathogenesis.

Language: Английский

Citations

1
A possible pathogenic role of Syndecan-1 in the pathogenesis of coronavirus disease 2019 (COVID-19) DOI Open Access
Sajad Karampoor, Hamid Zahednasab,

Mohammad Farahmand

et al.

International Immunopharmacology, Journal Year: 2021, Volume and Issue: 97, P. 107684 - 107684

Published: April 19, 2021

Language: Английский

Citations

50
HS, an Ancient Molecular Recognition and Information Storage Glycosaminoglycan, Equips HS-Proteoglycans with Diverse Matrix and Cell-Interactive Properties Operative in Tissue Development and Tissue Function in Health and Disease DOI Open Access
Anthony J. Hayes, James Melrose

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 1148 - 1148

Published: Jan. 6, 2023

Heparan sulfate is a ubiquitous, variably sulfated interactive glycosaminoglycan that consists of repeating disaccharides glucuronic acid and glucosamine are subject to number modifications (acetylation, de-acetylation, epimerization, sulfation). Variable heparan chain lengths sequences within the chains provide structural diversity generating oligosaccharide binding motifs with diverse range extracellular ligands cellular receptors providing instructional cues over behaviour tissue homeostasis through regulation essential physiological processes in development, health, disease. sulfate-PGs integral components specialized glycocalyx surrounding cells. most heterogeneous glycosaminoglycan, terms its sequence biosynthetic making it difficult molecule fully characterize, multiple also make an elucidation functional properties complicated. Spatio-temporal presentation groups important determinant development control wound healing remodelling pathological tissues. The regulatory mediated via interactions chemokines, chemokine receptors, growth factors morphogens cell proliferation, differentiation, remodelling, healing, immune regulation, inflammation, tumour development. A greater understanding these HS will improve therapeutic procedures prognoses. Advances synthesis sequencing, computational analytical carbohydrate algorithms advanced software for evaluation molecular docking partners now available. These analytic techniques artificial intelligence offer predictive capability conformational effects on sulfate-ligand significantly aiding therapeutics

Language: Английский

Citations

21
Mucin Glycans: A Target for Cancer Therapy DOI Creative Commons
Lingbo Sun, Yuhan Zhang, Wenyan Li

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(20), P. 7033 - 7033

Published: Oct. 11, 2023

Mucin glycans are an important component of the mucus barrier and a vital defence against physical chemical damage as well pathogens. There 20 mucins in human body, which can be classified into secreted transmembrane according to their distributions. The major difference between them is that do not have structural domains, expression each mucin organ cell-specific. Under physiological conditions, involved composition thus protect body from infection injury. However, abnormal lead occurrence diseases, especially cancer, through various mechanisms. Therefore, targeting for diagnosis treatment cancer has always been promising research direction. Here, we first summarize main types glycosylation (O-GalNAc N-glycosylation) on mechanisms by occur. Next, how contribute development described. Finally, MUC1-based antibodies, vaccines, radio-pharmaceuticals, CAR-T therapies using best characterized MUC1 example. In this section, specifically elaborate recent new therapy CAR-M, may bring hope patients.

Language: Английский

Citations

17
The Tumor Stroma of Squamous Cell Carcinoma: A Complex Environment That Fuels Cancer Progression DOI Open Access

Alexandra Buruiană,

Bogdan‐Alexandru Gheban,

Ioana‐Andreea Gheban‐Roșca

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(9), P. 1727 - 1727

Published: April 29, 2024

The tumor microenvironment (TME), a complex assembly of cellular and extracellular matrix (ECM) components, plays crucial role in driving progression, shaping treatment responses, influencing metastasis. This narrative review focuses on the cutaneous squamous cell carcinoma (cSCC) stroma, highlighting its key constituents their dynamic contributions. We examine how significant changes within cSCC ECM—specifically, alterations fibronectin, hyaluronic acid, laminins, proteoglycans, collagens—promote cancer metastasis, drug resistance. composition TME is also explored, detailing intricate interplay cancer-associated fibroblasts (CAFs), mesenchymal stem cells (MSCs), endothelial cells, pericytes, adipocytes, various immune populations. These diverse players modulate development, angiogenesis, responses. Finally, we emphasize TME’s potential as therapeutic target. Emerging strategies discussed this include harnessing system (adoptive transfer, checkpoint blockade), hindering disrupting CAF activity, manipulating ECM components. approaches underscore vital that deciphering interactions advancing therapy. Further research illuminating these relationships will uncover new avenues for developing more effective treatments cSCC.

Language: Английский

Citations

7
Indications of Persistent Glycocalyx Damage in Convalescent COVID-19 Patients: A Prospective Multicenter Study and Hypothesis DOI Creative Commons
Richard Vollenberg, Phil‐Robin Tepasse, Kevin Ochs

et al.

Viruses, Journal Year: 2021, Volume and Issue: 13(11), P. 2324 - 2324

Published: Nov. 21, 2021

The COVID-19 pandemic is caused by the SARS CoV-2 virus and can lead to severe lung damage hyperinflammation. In context of infection, inflammation-induced degradation glycocalyx layer in endothelial cells has been demonstrated. Syndecan-1 (SDC-1) an established parameter for measuring injury. This prospective, multicenter, observational, cross-sectional study analyzed SDC-1 levels 24 convalescent patients that had infected with SARS-CoV-2 mild disease course without need hospitalization. We included 13 age-matched healthy individuals 10 hospitalized acute as controls. patients, significantly elevated were detected after a median 88 days symptom onset compared controls, whereas no difference was found when undergoing disease. first demonstrate signs non-pre-diseased, progression data are consistent studies showing evidence persistent or critical progression. Further work investigate should follow.

Language: Английский

Citations

34
Glycosaminoglycans in cancer therapy DOI

Ronja Wieboldt,

Heinz Laübli

AJP Cell Physiology, Journal Year: 2022, Volume and Issue: 322(6), P. C1187 - C1200

Published: April 6, 2022

Glycosaminoglycans (GAGs) are an important component of the tumor microenvironment (TME). GAGs can interact with a variety binding partners and thereby influence cancer progression on multiple levels. modulate growth factors chemokine signaling, invasion, metastasis formation. Moreover, able to change physical property extracellular matrix (ECM). Abnormalities in GAG abundance structure (e.g., sulfation patterns molecular weight) found across various types show biomarker potential. Targeting GAGs, as well usage their mimetics, promising approaches interfere progression. In addition, be used drug cytokine carriers induce antitumor response. this review, we summarize role potential use derivatives target cancer.

Language: Английский

Citations

25