Ingested Polystyrene Microplastics as a Carrier of Heavy Metals (Cadmium or Silver): Uptake, Gut Damage, Oxidative Stress, and DNA Damage In Drosophila Larvae DOI
Eşref Demir, Fatma Turna Demir, Gökhan Akkoyunlu

et al.

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

Living organisms are now constantly exposed to microplastics and nanoplastics (MNPLs), besides their huge toxic potential, they can also act as carriers of various hazardous elements like heavy metals. Therefore, this study explored possible interactions between polystyrene (PSMPLs) two metal pollutants: cadmium chloride (CdCl2) silver nitrate (AgNO3). To better understand the extent biological effects caused by different sizes PSMPLs, we conducted in vivo experiments with five doses (from 0.01 10 mM) that contained particles measuring 4, 10, 20 µm size on Drosophila larvae adult flies. Additional were performed exposing flies individual metals CdCl2 (0.5 AgNO3 mM), well combined exposure PSMPLs (0.01, these metals, an attempt gain new insight into health risks such coexposure. Using transmission electron microscopy imaging, managed visualize journey ingested throughout fly’s body, observing plastics intestinal lumen, cellular uptake gut enterocytes, passage plastic through barrier leak hemolymph, hemocytes. Observations detected shape changes PSMPLs. Egg-to-adult viability screening revealed no significant toxicity upon tested materials, however induced aggravated genotoxic effects, including damage, genetic intracellular oxidative stress (ROS generation), smaller sized + (cadmium silver) causing greater damage.

Language: Английский

Hazard assessment of ingested polystyrene nanoplastics in Drosophila larvae DOI Creative Commons
Mohamed Alaraby, Doaa Abass, Josefa Domenech

et al.

Environmental Science Nano, Journal Year: 2022, Volume and Issue: 9(5), P. 1845 - 1857

Published: Jan. 1, 2022

Micro- and nanoplastics (MNPLs) are intentionally produced for commercial uses (primary MNPLs) or formed from environmentally aged plastics (secondary MNPLs).

Language: Английский

Citations

26
Interactions of Ingested Polystyrene Microplastics with Heavy Metals (Cadmium or Silver) as Environmental Pollutants: A Comprehensive In Vivo Study Using Drosophila melanogaster DOI Creative Commons
Fatma Turna Demir, Gökhan Akkoyunlu, Eşref Demir

et al.

Biology, Journal Year: 2022, Volume and Issue: 11(10), P. 1470 - 1470

Published: Oct. 8, 2022

Living organisms are now constantly exposed to microplastics and nanoplastics (MNPLs), besides their toxic potential, they can also act as carriers of various hazardous elements such heavy metals. Therefore, this study explored possible interactions between polystyrene (PSMPLs) two metal pollutants: cadmium chloride (CdCl

Language: Английский

Citations

24
BRCA2 deficiency reveals that oxidative stress impairs RNaseH1 function to cripple mitochondrial DNA maintenance DOI Creative Commons
Xavier Renaudin, Mi-Young Lee, Mona Shehata

et al.

Cell Reports, Journal Year: 2021, Volume and Issue: 36(5), P. 109478 - 109478

Published: Aug. 1, 2021

Oxidative stress is a ubiquitous cellular challenge implicated in aging, neurodegeneration, and cancer. By studying pathogenic mutations the tumor suppressor BRCA2, we identify general mechanism by which oxidative restricts mitochondrial (mt)DNA replication. BRCA2 inactivation induces R-loop accumulation mtDNA regulatory region diminishes replication initiation. In BRCA2-deficient cells, intracellular reactive oxygen species (ROS) are elevated, ROS scavengers suppress defects. Conversely, wild-type cells exposed to pharmacologic or genetic manipulation phenocopy these Mechanistically, find that 8-oxoguanine caused suffices impair recruitment of enzyme RNaseH1 sites accrual, restricting Thus, impairs function cripple maintenance. Our findings highlight molecular links dysfunction elicited genes neurodegeneration

Language: Английский

Citations

28
Dynamic changes of DNA methylation induced by benzo(a)pyrene in cancer DOI Creative Commons

Huizeng Wang,

Bingchun Liu, Hong Chen

et al.

Genes and Environment, Journal Year: 2023, Volume and Issue: 45(1)

Published: July 1, 2023

Benzo(a)pyrene (BaP), the earliest and most significant carcinogen among polycyclic aromatic hydrocarbons (PAHs), has been found in foods, tobacco smoke, automobiles exhaust, etc. Exposure to BaP induced DNA damage directly, or oxidative stress-related damage, resulting cell apoptosis carcinogenesis human respiratory system, digestive reproductive Moreover, triggered genome-wide epigenetic alterations by methylation, which might cause disturbances regulation of gene expression, thereby cancer. It proved that reduced activated proto-oncogene hypomethylation promoter region, but silenced tumor suppressor genes hypermethylation, cancer initiation progression. Here we summarized changes methylation exposure, revealed plays a role development.

Language: Английский

Citations

12
The role of DNA and RNA guanosine oxidation in cardiovascular diseases DOI Creative Commons
Yiping Li, Xiaolong Wang

Pharmacological Research, Journal Year: 2024, Volume and Issue: 204, P. 107187 - 107187

Published: April 23, 2024

Cardiovascular diseases (CVD) persist as a prominent cause of mortality worldwide, with oxidative stress constituting pivotal contributory element. The modification guanosine, specifically 8-oxoguanine, has emerged crucial biomarker for stress, providing novel insights into the molecular underpinnings CVD. 8-Oxoguanine can be directly generated at DNA (8-oxo-dG) and RNA levels, well free nucleotide level (8-oxo-dGTP or 8-oxo-GTP), which are produced integrated through replication transcription. When exposed to guanine is more readily in than DNA. A burgeoning body research surrounds exhibits its accumulation playing role development Therapeutic approaches targeting damage RNA, encompassing modulation repair enzymes small molecule inhibitors, anticipated enhance CVD management. In conclusion, we explore noteworthy elevation 8-oxoguanine levels patients various cardiac conditions deliberate upon formation regulation 8-oxo-dG 8-oxo-G under their function

Language: Английский

Citations

5
Application of artificial intelligence for detection of chemico-biological interactions associated with oxidative stress and DNA damage DOI

Lazar M. Davidovic,

Darko Laketić,

Jelena Cumic

et al.

Chemico-Biological Interactions, Journal Year: 2021, Volume and Issue: 345, P. 109533 - 109533

Published: May 27, 2021

Language: Английский

Citations

24
The mutagenic consequences of defective DNA repair DOI Creative Commons
Eszter Németh, Dávid Szüts

DNA repair, Journal Year: 2024, Volume and Issue: 139, P. 103694 - 103694

Published: May 18, 2024

Multiple separate repair mechanisms safeguard the genome against various types of DNA damage, and their failure can increase rate spontaneous mutagenesis. The malfunction distinct leads to genomic instability through different mutagenic processes. For example, defective mismatch causes high base substitution rates microsatellite instability, whereas homologous recombination deficiency is characteristically associated with deletions chromosome instability. This review presents a comprehensive collection all phenotypes loss each mechanism, drawing on data from variety model organisms mutagenesis assays, placing greatest emphasis systematic analyses human cancer datasets. We describe latest theories mechanism process, often explained by reliance an alternative pathway or error-prone replication unrepaired, damaged DNA. Aided concept mutational signatures, be used in diagnosis identify pathways.

Language: Английский

Citations

3
Simultaneous removal of microorganisms and ethyl acetate from fruit storage indoor air using a low-cost photoreactor based on luminous textiles DOI
Sarra Karoui, Aymen Amine Assadi, Amina Meslem

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104771 - 104771

Published: July 20, 2024

Language: Английский

Citations

3
Broad repression of DNA repair genes in senescent cells identified by integration of transcriptomic data DOI Creative Commons
Yann Frey, Majd Haj, Yael Ziv

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Cellular senescence plays a significant role in tissue aging. Senescent cells, which resist apoptosis while remaining metabolically active, generate endogenous DNA-damaging agents, primarily reactive oxygen species. Efficient DNA repair is therefore crucial these especially when they undergo escape, resuming replication and cellular proliferation. To investigate whether senescent cell transcriptomes reflect adequate capacity, we conducted comprehensive meta-analysis of 60 transcriptomic datasets comparing to proliferating cells. Our analysis revealed striking downregulation genes encoding essential components across pathways This includes active different cycle phases such as nucleotide excision repair, base nonhomologous end joining homologous recombination double-strand breaks, mismatch interstrand crosslink repair. The observed suggests accumulation lesions. Experimental monitoring readouts cells that underwent radiation-induced supported this conclusion. phenomenon was consistent various triggers also primary lines from aging individuals. These findings highlight the potential ‘ticking bombs’ aging-related diseases tumors recurring following therapy-induced senescence.

Language: Английский

Citations

2
DNA polymerase eta protects human cells against DNA damage induced by the tumor chemotherapeutic temozolomide DOI Creative Commons
Marcela Teatin Latância, Natália Cestari Moreno, Giovana da Silva Leandro

et al.

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Journal Year: 2022, Volume and Issue: 878, P. 503498 - 503498

Published: May 5, 2022

Language: Английский

Citations

9