Biomolecular Markers of Recurrent Implantation Failure—A Review

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(18), P. 10082 - 10082

Published: Sept. 18, 2021

Currently, infertility affects 8–12% of reproductive age couples worldwide, a problem that also women suffering from recurrent implantation failure (RIF). RIF is complex condition resulting many physiological and molecular mechanisms involving dynamic endometrium–blastocyst interaction. The most important are the endometrial receptivity process, decidualization, trophoblast invasion, blastocyst nesting. Although exact multifactorial pathogenesis remains unclear, studies have suggested association between hormone level imbalance, disturbances angiogenic immunomodulatory factors, certain genetic polymorphisms, occurrence RIF. These were performed in quite small groups. Additionally, results inconsistent ethnicities. present review briefly summarizes importance factors involved development could serve as diagnostic determinants. Moreover, our constitute part new platform for discovery novel therapeutic solutions

Language: Английский

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Characterization of an artificial intelligence model for ranking static images of blastocyst stage embryos

Fertility and Sterility, Journal Year: 2022, Volume and Issue: 117(3), P. 528 - 535

Published: Jan. 5, 2022

ObjectiveTo perform a series of analyses characterizing an artificial intelligence (AI) model for ranking blastocyst-stage embryos. The primary objective was to evaluate the benefit predicting clinical pregnancy, whereas secondary identify limitations that may impact use.DesignRetrospective study.SettingConsortium 11 assisted reproductive technology centers in United States.Patient(s)Static images 5,923 transferred blastocysts and 2,614 nontransferred aneuploid blastocysts.Intervention(s)None.Main Outcome Measure(s)Prediction pregnancy (fetal heartbeat).Result(s)The area under curve AI ranged from 0.6 0.7 outperformed manual morphology grading overall on per-site basis. A bootstrapped study predicted improved rates between +5% +12% per site using compared with inverted microscope. One used low-magnification stereo zoom microscope did not show improvement AI. Visualization techniques attribution algorithms revealed features learned by largely overlap systems. Two sources bias relating type presence embryo holding micropipettes were identified mitigated. analysis scores relation showed score differences ≥0.1 (10%) correspond rates, <0.1 be clinically meaningful.Conclusion(s)This demonstrates potential blastocyst stage embryos highlights related image quality, bias, granularity scores. To use. Retrospective study. Consortium States. Static blastocysts. None. Prediction heartbeat). meaningful. This

Language: Английский

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The value of intraovarian autologous platelet rich plasma in women with poor ovarian reserve or ovarian insufficiency: a systematic review and meta-analysis

BMC Pregnancy and Childbirth, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 27, 2024

Abstract Objectives To assess the value of intraovarian PRP in women with low ovarian reserve. Search strategy Screening databases from inception to January 2023 using keywords related “Platelet-rich plasma” AND “poor reserve” OR “ovarian failure”. Selection criteria Fourteen studies (1632 participants) were included, 10 included POR, 1 POI and 3 both POR women. Data collection analysis Extracted data study settings, design, sample size, population characteristics, volume, timing preparation administration, outcome parameters. Main results AMH level was evaluated 11 (2099 women). The mean difference (MD) 0.09 95% CI – 0.06, 0.24 ( P = 0.25). Antral follicular count assessed 6 (1399 MD 1.73 0.81, 2.66 < 0.001). number oocytes retrieved 7 (1413 1.21 0.48, 1.94 Conclusion This systematic review found a significant improvement AFC, oocytes, cleavage embryos cancellation rate POR. Trial registration Registration CRD42022365682.

Language: Английский

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An endometrial receptivity scoring system basing on the endometrial thickness, volume, echo, peristalsis, and blood flow evaluated by ultrasonography

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 9, 2022

Establishing a successful pregnancy depends on the endometrium and embryo. It is estimated that suboptimal endometrial receptivity account for one-third of implantation failures. Despite indepth understanding processes associated with embryo-endometrial cross-talk, little progress has been achieved diagnosis treatments receptivity.This retrospective study included women undergoing their first frozen-thawed embryo transfer (FET) cycles at our reproductive medicine center from March 2021 to August 2021. Transvaginal three-dimensional (3D) ultrasound was performed in morning day all thawed patients, evaluate receptivity, including thickness, echogenicity, volume, movement blood flow.A total number 562 patients FET 315 pregnancies (56.0%) analyzed. found only echo central line different between pregnant group non-pregnant group. Other parameters, such as peristalsis, or flow were not statistically two groups. Then, according relationship groups clinical rate, score 0 2 respectively scored. The sum scores six items patient's score. showed rate increased increased, when reaches least 5, significantly improved (63.7% versus 49.5%, P=0.001).We developed an scoring system demonstrated its validity. may aid clinicians choosing useful marker practice informing further research.

Language: Английский

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The value of platelet-rich plasma in women with previous implantation failure: a systematic review and meta-analysis

Journal of Assisted Reproduction and Genetics, Journal Year: 2023, Volume and Issue: 40(5), P. 969 - 983

Published: April 3, 2023

Abstract Objective To assess the value of intrauterine PRP to improve IVF outcome in women with previous implantation failure. Methods Screening Pubmed, Web Science, and other databases from inception August 2022 using keywords related “platelet-rich plasma” OR “PRP” AND “IVF” “implantation failure.” Twenty-nine studies (3308 participants) were included our analysis, 13 RCTs, 6 prospective cohorts, 4 single arm, retrospective analyses. Extracted data settings study, study type, sample size, participants’ characteristics, route, volume, timing administration, parameters. Results Implantation rate was reported RCTs (886 non-RCTs (732 participants). The odds ratio (OR) effect estimate 2.62 2.06, 95% CI 1.83, 3.76, 1.03–4.11, respectively. Endometrial thickness compared (307 9 (675 participants), which showed a mean difference 0.93 1.16, 0.59–1.27 0.68–1.65 CI, Conclusion administration improves implantation, clinical pregnancy, chemical ongoing live birth rates, endometrial

Language: Английский

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The composition of menstrual fluid, its applications, and recent advances to understand the endometrial environment: a narrative review

F&S Reviews, Journal Year: 2024, Volume and Issue: 5(3), P. 100075 - 100075

Published: May 24, 2024

Research into menstrual fluid (MF), also referred to as blood or effluent in the literature, and its potential a diagnostic tool has been largely overlooked. The current understanding of endometrium principally derived from invasive techniques, such endometrial biopsies, yet tissue is cyclically shed MF. Menstrual composition that distinctly reflects environment provides an easily accessible source constituents can be collected relatively noninvasively using cup. offers renewable inexpensive option replace more uncomfortable methods collecting samples. health provide invaluable insight development disorders. recently utilized research pathogenesis endometriosis, chronic endometritis, unexplained infertility; however, some logistical challenges recruitment participants, collection, processing MF samples require optimization. This review detailed what we currently know about recent advances research, well future directions. unique window endometrium, further could help uncover disorders have exciting prospects tool.

Language: Английский

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The Complicated Effects of Extracellular Vesicles and Their Cargos on Embryo Implantation

Frontiers in Endocrinology, Journal Year: 2021, Volume and Issue: 12

Published: June 4, 2021

As a rate-limiting step in pregnancy, embryo implantation is highly dependent on intercellular communication. Extracellular vesicles (EVs) are newly identified to be important the course of EVs have been isolated from wide variety biofluids and tissues, including plasma, liver, uterine, semen, embryo, etc. The present future use not only as biomarkers, but also targeting drug delivery system, promisingly pave way for advanced comprehension failure reproductive diseases. However, precise mechanisms has elucidated yet. Herein, we summarize current knowledge diverse effects various sources their cargos such microRNA, long non-coding RNA, protein, implantation, potential diseases recurrent failure, polycystic ovary syndrome endometriosis. It essential note that many biologically plausible functions discussed literatures still need further research vivo .

Language: Английский

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Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(9), P. 2481 - 2481

Published: April 28, 2022

An abnormal endometrial microbiota has been suggested to impair the process of embryo implantation, thus leading repeated implantation failure (RIF) in women undergoing vitro fertilization (IVF). However, molecular mechanisms linking uterine and IVF out-comes are still an open question. The aim this cohort study was outline relationship between microbiota, inflammation outcomes. To purpose, selected components “cytokine network” were analyzed presenting RIF divided eubiosis dysbiosis groups, according percentage lactobacilli (≥90% or <90%, respectively). Dysbiosis group presented significantly higher tissue concentrations inflammatory markers (IL-6, IL-1β, HIF-1α COX-2) lower levels anti-inflammatory/well-being factors, IL-10 IGF-1, with respect eubiosis. Moreover, Lactobacillus negatively related molecules positively IL-10/IGF-1. Interestingly, number attempts directly factors COX-2, IL-1β group. Overall, demonstrated be associated inflammation-related changes affecting underlining importance assessing patients IVF.

Language: Английский

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Targeted gene expression profiling for accurate endometrial receptivity testing

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Aug. 26, 2023

Abstract Expressional profiling of the endometrium enables personalised timing window implantation (WOI). This study presents and evaluates a novel analytical pipeline based on TAC-seq (Targeted Allele Counting by sequencing) method for endometrial dating. The expressional profiles were clustered, differential expression analysis was performed model development group, using 63 biopsies spanning over proliferative (PE, n = 18), early-secretory (ESE, mid-secretory (MSE, 17) late-secretory (LSE, 10) phases natural cycle. A quantitative predictor trained group validated sequenced samples from healthy women, consisting 52 paired taken ESE MSE five LSE phase 31 individuals. Finally, developed test applied to 44 patients diagnosed with recurrent failure (RIF). In validation (n 57), we detected displaced WOI in 1.8% fertile women. RIF significantly higher proportion shifted than set 15.9% (p 0.012), respectively. evaluated an average cross-validation accuracy 98.8% 98.2% group. beREADY screening sensitive dynamic detection selected transcriptome biomarkers, providing accurate prediction receptivity status.

Language: Английский

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Endometrium Immunomodulation to Prevent Recurrent Implantation Failure in Assisted Reproductive Technology

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 12787 - 12787

Published: Oct. 24, 2022

After more than four decades of assisted reproductive technology (ART) practice worldwide, today 60% women undergoing in vitro fertilization (IVF) treatments fail to become pregnant after the first embryo transfer and nearly 20% patients are suffering from unexplained recurrent implantation failures (RIFs) repeated pregnancy loss (RPL). The literature reported different causes RIF–RPL, mainly multifactorial, endometrial idiopathic. RIF remains a black box because complicated categorization this physio-pathological dysregulation process ovarian stimulation. Many options were suggested as solutions treat RIF–RPL with controversial results on their usefulness. In article, we reviewed possible therapeutic improve rates clinical outcomes. Based our experience believe that endometrium immunomodulation intrauterine insemination activated autologous peripheral blood mononuclear cells (PBMCs) or platelet-rich plasma (PRP) can be promising solution. On other hand, lymphocyte balance typing, specific cytokines interleukins profiling proposed predictive biomarkers before transfer.

Language: Английский

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