BioJect: An in vitro platform to explore release dynamics of peptides in subcutaneous drug delivery

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 1058 - 1079

Published: Feb. 24, 2025

Language: Английский

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Exploring Physiological Differences in Injury Response by Skin Tone: A Scoping Review

Journal of Tissue Viability, Journal Year: 2025, Volume and Issue: 34(2), P. 100871 - 100871

Published: Feb. 14, 2025

To explore existing literature examining physiological differences in pressure ulcer response among individuals with differing skin tones. This was a scoping review. Articles meeting the inclusion criteria were retrieved from electronic databases including PubMed, CINAHL, Scopus, Cochrane, and EMBASE, using keywords "pressure ulcer," "skin pigmentation," "melanin," "risk factor." Data extracted predesigned data extraction tool analysed narrative synthesis. Five papers met criteria. Analysis of findings suggests there are potential mechanisms which may influence skin's ability to withstand mechanical stress its inflammatory damage those different tones; structure stratum corneum, collagen density, fibroblast activity, mast cell transepidermal water loss (TEWL). The corneum can compromise resilience, while density activity impact strength repair. Mast cells affect inflammation, exacerbate damage, increased TEWL dark tones result lower content affecting hydration.Conversely, factors like melanosome size, hair follicle fiber characteristics, sebaceous gland vitamin D production, UVR protection, desquamation rate, although relevant overall health, not directly processes leading formation. Physiological contribute alterations development skin. Recognising these is important for targeted prevention strategies within diverse populations. However, further research needed underlying this association greater detail.

Language: Английский

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In Vitro and Clinical Evaluation of Cannabigerol (CBG) Produced via Yeast Biosynthesis: A Cannabinoid with a Broad Range of Anti-Inflammatory and Skin Health-Boosting Properties

Molecules, Journal Year: 2022, Volume and Issue: 27(2), P. 491 - 491

Published: Jan. 13, 2022

Cannabigerol (CBG) is a minor non-psychoactive cannabinoid present in Cannabis sativa L. (C. sativa) at low levels (<1% per dry weight) that serves as the direct precursor to both cannabidiol (CBD) and tetrahydrocannabinol (THC). Consequently, efforts extract purify CBG from C. challenging expensive. However, utilizing novel yeast fermentation technology platform, cannabinoids such can be produced more sustainable, cost-effective, timely process compared plant-based production. While CBD has been studied extensively, demonstrating several beneficial skin properties, there are paucity of studies characterizing activity human skin. Therefore, our aim was characterize compare vitro profile first group test clinically on Gene microarray analysis conducted using 3D equivalents demonstrates regulates genes than CBD, including key targets. Human dermal fibroblasts (HDFs) normal epidermal keratinocytes (NHEKs) were exposed culture pro-inflammatory inducers trigger cytokine production oxidative stress. Results demonstrate reduce reactive oxygen species HDFs better vitamin Moreover, inhibits (Interleukin-1β, -6, -8, tumor necrosis factor α) release inflammatory inducers, ultraviolet A (UVA), B (UVB), chemical, acnes, instances does so potently CBD. 20-subject vehicle-controlled clinical study performed with 0.1% serum placebo applied topically for 2 weeks after sodium lauryl sulfate (SLS)-induced irritation. showed statistically significant improvement above transepidermal water loss (TEWL) reduction appearance redness. Altogether, CBG's broad range health-promoting activities its strong potential safe, effective ingredient topical use suggests areas where it may

Language: Английский

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Extracellular matrix stiffness—The central cue for skin fibrosis

Frontiers in Molecular Biosciences, Journal Year: 2023, Volume and Issue: 10

Published: March 8, 2023

Skin fibrosis is a physiopathological process featuring the excessive deposition of extracellular matrix (ECM), which main architecture that provides structural support and constitutes microenvironment for various cellular behaviors. Recently, increasing interest has been drawn to relationship between mechanical properties ECM initiation modulation skin fibrosis, with engagement complex network signaling pathways, activation mechanosensitive proteins, changes in immunoregulation metabolism. Simultaneous progression stiffness increases, turn perturbs humoral homeostasis drive cell fate toward an outcome maintains enhances process, thus forming pro-fibrotic "positive feedback loop". In this review, we highlighted central role its dynamic at both molecular levels fibrosis. We paid special attention pathways regulated by cues remodeling. also systematically summarized antifibrotic interventions targeting ECM, hopefully enlightening new strategies fibrotic diseases.

Language: Английский

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Autophagy is the key to making chronic wounds acute in skin wound healing

Autophagy, Journal Year: 2023, Volume and Issue: 19(9), P. 2578 - 2584

Published: March 30, 2023

As a highly regulated and dynamically balanced intracellular degradation mechanism, macroautophagy/autophagy plays an essential housekeeping role in different successive stages of skin wound healing; from the homeostasis inflammatory to proliferative remodeling stages. Under both progressive defective healing conditions, autophagy operates at levels with precise extent activity, interface inflammation, stress signaling cell metabolism through complex spatiotemporal cascade molecular cellular events. Depending on conditions autophagic activity is fine-tuned differentially modulated each stage order cope stage-specific requirements. Here, we postulate that under favorable may act as key modulator by making chronic wounds acute. Enhancing topical application pro-autophagy biologics appropriate hydrating vehicle/moisturizing base such hydrogels, onto provide moisture immune modulation, thus contributing rapid efficient healing. A moist environment more conducive it helps not only accelerate proliferation migration, extracellular matrix reorganization, but also promotes reduces incidence inflammation.

Language: Английский

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The Tumor Stroma of Squamous Cell Carcinoma: A Complex Environment That Fuels Cancer Progression

Cancers, Journal Year: 2024, Volume and Issue: 16(9), P. 1727 - 1727

Published: April 29, 2024

The tumor microenvironment (TME), a complex assembly of cellular and extracellular matrix (ECM) components, plays crucial role in driving progression, shaping treatment responses, influencing metastasis. This narrative review focuses on the cutaneous squamous cell carcinoma (cSCC) stroma, highlighting its key constituents their dynamic contributions. We examine how significant changes within cSCC ECM—specifically, alterations fibronectin, hyaluronic acid, laminins, proteoglycans, collagens—promote cancer metastasis, drug resistance. composition TME is also explored, detailing intricate interplay cancer-associated fibroblasts (CAFs), mesenchymal stem cells (MSCs), endothelial cells, pericytes, adipocytes, various immune populations. These diverse players modulate development, angiogenesis, responses. Finally, we emphasize TME’s potential as therapeutic target. Emerging strategies discussed this include harnessing system (adoptive transfer, checkpoint blockade), hindering disrupting CAF activity, manipulating ECM components. approaches underscore vital that deciphering interactions advancing therapy. Further research illuminating these relationships will uncover new avenues for developing more effective treatments cSCC.

Language: Английский

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Dermatologic Changes in Experimental Model of Long COVID

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 272 - 272

Published: Jan. 27, 2024

The coronavirus disease-19 (COVID-19) pandemic, declared in early 2020, has left an indelible mark on global health, with over 7.0 million deaths and persistent challenges. While the pharmaceutical industry raced to develop vaccines, emergence of mutant severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) strains continues pose a significant threat. Beyond immediate concerns, long-term health repercussions COVID-19 survivors are garnering attention, particularly due documented cases cardiovascular issues, liver dysfunction, pulmonary complications, kidney impairments, notable neurocognitive deficits. Recent studies have delved into pathophysiological changes various organs following post-acute infection murine hepatitis virus-1 (MHV-1), coronavirus, mice. One aspect that stands out is impact skin, previously underexplored facet effects. research reveals cutaneous findings during both phases post-MHV-1 infection, mirroring certain alterations observed humans post-SARS-CoV-2 infection. In stages, mice exhibited destruction epidermal layer, increased hair follicles, extensive collagen deposition dermal hyperplasticity sebaceous glands. Moreover, thinning panniculus carnosus adventitial layer was noted, consistent human studies. A investigation revealed absence adipose tissues, further damage layer. Remarkably, treatment synthetic peptide, SPIKENET (SPK), designed prevent Spike glycoprotein-1 binding host receptors elicit potent anti-inflammatory response, showed protection against MHV-1 Precisely, SPK restored follicle loss re-architected layers, successfully overhauled fatty tissue destruction. These promising underscore potential as therapeutic intervention skin initiated by SARS-CoV-2, providing glimmer hope battle lingering effects pandemic.

Language: Английский

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Smart Tissue Carriers for Innovative Cosmeceuticals and Nutraceuticals

Cosmetics, Journal Year: 2024, Volume and Issue: 11(1), P. 20 - 20

Published: Feb. 2, 2024

The present review was conducted to investigate the possibilities in realizing novel nanostructured tissues containing functional molecules that can be commercialized as solid products (without using emulsifiers and preservatives) for cosmeceutical nutraceutical applications. After considering principal concepts regarding skin mucous features physiologies, bio-based, biodegradable biocompatible materials explored by investigating correlations between their structures morphologies with respect characteristics of extracellular matrix (ECM). Regarding new smart type tissues, possible composition reviewed relation aging process current contest novel, innovative cosmeceuticals nutraceuticals consider “beauty from within” concept. barriers development these were mainly identified due necessity defining claim green products. Moreover, market growth data highlighted support idea diffusion tissue-based is an opportunity sustainable industrial chains bioeconomies.

Language: Английский

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Skin-on-a-chip technologies towards clinical translation and commercialization

Biofabrication, Journal Year: 2024, Volume and Issue: 16(4), P. 042001 - 042001

Published: July 4, 2024

Skin is the largest organ of human body which plays a critical role in thermoregulation, metabolism (e.g. synthesis vitamin D), and protection other organs from environmental threats, such as infections, microorganisms, ultraviolet radiation, physical damage. Even though skin diseases are considered to be less fatal, ubiquity irritation caused by them highlights importance studies. Furthermore, promising means for transdermal drug delivery, requires thorough understanding structure. Current animal andin vitrotwo/three-dimensional models provide platform disease studies testing, whereas they face challenges complete recapitulation dynamic complex structure actual tissue. One most effective methods testing pharmaceuticals modeling skin-on-a-chip (SoC) platforms. SoC technologies non-invasive approach examining 3D layers artificially creating order develop diagnostic or therapeutic methods. In addition, enable perfusion culture medium with nutrients facilitate continuous removal cellular waste further mimic thein vivocondition. Here, article reviews recent advances design applications platforms well analysis drugs cosmetics. By contributions different patents physiological relevance models, review underscores significant shift towards more ethical efficient alternatives testing. it explores market dynamics ofin vitroskin organ-on-a-chip platforms, discussing impact legislative changes demand on development adoption these advanced research tools. This also identifies existing obstacles that hinder advancement proposing directions future improvements, particularly focusing journey clinical adoption.

Language: Английский

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Mechanotransduction in Skin Inflammation

Cells, Journal Year: 2022, Volume and Issue: 11(13), P. 2026 - 2026

Published: June 25, 2022

In the process of mechanotransduction, cells in body perceive and interpret mechanical stimuli to maintain tissue homeostasis respond environmental changes. Increasing evidence points towards dysregulated mechanotransduction as a pathologically relevant factor human diseases, including inflammatory conditions. Skin is organ that constantly undergoes considerable stresses, ability factors provoke processes skin has long been known, with Koebner phenomenon being an example. However, molecular mechanisms key linking cutaneous inflammation remain understudied. this review, we outline players tissue's homeostasis, available data, gaps our current understanding their aberrant regulation chronic inflammation. We mainly focus on psoriasis one most studied diseases; also discuss context fibrosis result Even though role simple epithelia internal organs actively studied, conclude mechanoregulation stratified epidermis requires more attention future translational research.

Language: Английский

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