Exosomes promote axon outgrowth by engaging the Wnt-Planar Cell Polarity pathway DOI Open Access
Samar Ahmad,

Melanie Pye,

Masahiro Narimatsu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 28, 2023

In neurons, the acquisition of a polarized morphology is achieved upon outgrowth single axon from one several neurites. Exosomes or small extracellular vesicles (sEVs) diverse sources are known to promote neurite and thus may have therapeutic potential. However, effect fibroblast-derived exosomes on elongation in neurons central nervous system under growth permissive conditions remains unclear. Here, we show that sEVs neuronal mouse primary embryonic cortical neurons. Mechanistically, demonstrate sEV-induced increase requires endogenous Wnts core PCP components including Prickle, Vangl, Frizzled Dishevelled. We internalized by colocalize with Wnt7b induce relocalization Vangl2 distal during outgrowth. contrast, derived astrocytes do not outgrowth, while activated inhibit elongation. Thus, our data reveals through Wnt-PCP pathway manner dependent Wnts.

Language: Английский

Nuclear Dishevelled: An enigmatic role in governing cell fate and Wnt signaling DOI Creative Commons

Geetha P. Boligala,

Mingxiao V. Yang,

Jenna C. van Wunnik

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2022, Volume and Issue: 1869(10), P. 119305 - 119305

Published: June 8, 2022

The Dishevelled gene was first identified in Drosophila mutants with disoriented hair and bristle polarity subsequent work has now demonstrated its importance critical diverse aspects of biology. Since those early discoveries, been shown to coordinate a plethora developmental cellular processes that range from controlling cell during gastrulation partnering chromatin modifying enzymes regulate histone methylation at genomic loci. While the role DVL development is well-respected cytosolic function studied more extensively, nuclear continues remain murky. In this review we highlight some seminal discoveries have contributed field, but primary focus discuss recent advances respect Dishevelled. This dimension which proving be increasingly important yet remains enigmatic.

Language: Английский

Citations

12

Gene Expression Analysis of the Stress Response to Lithium, Nickel, and Zinc in Paracentrotus lividus Embryos DOI Creative Commons
Rosa Bonaventura, Caterina Costa, Irene Deidda

et al.

Toxics, Journal Year: 2022, Volume and Issue: 10(6), P. 325 - 325

Published: June 14, 2022

Many anthropogenic pollutants such as metals are discharged into the marine environment through modern sources. Among these, lithium (Li), nickel (Ni), and zinc (Zn) can interfere with biological processes in many organisms when their concentration rises. These toxic to sea urchin embryos, affecting development. Indeed, animal/vegetal dorso/ventral embryonic axes differently perturbed: Li is a vegetalizing agent, Ni disrupt dorso-ventral axis, Zn be animalizing. To address molecular response adopted by embryos cope these or involved gene networks regulating embryogenesis, detect new biomarkers for evaluating hazards polluted environments well-known vivo model, we applied high-throughput screening approach embryos. After fertilization, Paracentrotus lividus were exposed Li, Ni, 24/48 h. At both endpoints, RNAs analyzed NanoString nCounter technology. By silico analyses, selected panel of 127 transcripts encoding regulatory structural proteins, ranked categories: Apoptosis, Defense, Immune, Nervous, Development, Biomineralization. The data analysis highlighted dysregulation genes metal-dependent manner. A functional annotation was performed KEEG Orthology database. This study provides platform research on urchins.

Language: Английский

Citations

11

Identification of candidate genes and pathways involved in the establishment of sexual size dimorphism in the olive flounder (Paralichthys olivaceus) using RNA-seq DOI
Yuqing Zhang, Wenying Zhang,

Yanmin Jian

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 595, P. 741604 - 741604

Published: Sept. 10, 2024

Language: Английский

Citations

2

Precious but convenient means of prevention and treatment: physiological molecular mechanisms of interaction between exercise and motor factors and Alzheimer’s disease DOI Creative Commons
Zikang Hao, Kerui Liu, Lu Zhou

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: June 8, 2023

Disproportionate to the severity of Alzheimer’s disease (AD) and huge number patients, exact treatment prevention AD is still being explored. With increasing ageing, search for means prevent treat has become a high priority. In AD, it been suggested that exercise may be one more effective less costly preventing treating therefore large part current research aimed at exploring effectiveness in AD. However, due complexity specific pathogenesis there are multiple hypotheses potential mechanisms interventions need This review specifically summarises interaction between from molecular perspective, based on available evidence animal models or human experiments, explores them categorised according pathologies associated with AD: can activate signalling pathways inhibited by (e.g., Wnt PI3K/Akt pathways) reactivate effects downstream factors regulated these pathways, thus acting alleviate autophagic dysfunction, relieve neuroinflammation mitigate Aβ deposition. addition, this paper introduces new approach regulate blood-brain barrier, i.e., restore stability reduce abnormal phosphorylation tau proteins neuronal apoptosis. concept.” Motor factors” “Exerkines”, which act through autocrine, paracrine endocrine stimulation response movement. process, we believe great three areas: (1) alleviation movement brain-gut axis (2) combined polyphenols (3) continued exploration movement-mediated activation pathway

Language: Английский

Citations

4

Targeting WNT signalling pathways as new therapeutic strategies for osteoarthritis DOI

Zoya Iqbal,

Jiang Xia, Ghulam Murtaza

et al.

Journal of drug targeting, Journal Year: 2023, Volume and Issue: 31(10), P. 1027 - 1049

Published: Nov. 16, 2023

Osteoarthritis (OA) is a highly prevalent chronic joint disease and the leading cause of disability. Currently, no drugs are available to control damage or ease associated pain. The wingless-type (WNT) signalling pathway vital in OA progression. Excessive activation WNT pertinent progression severity. Therefore, agonists antagonists considered potential drug candidates for treatment. For example, SM04690, novel small molecule inhibitor signalling, has demonstrated its recent phase III clinical trial as disease-modifying osteoarthritis (DMOAD). targeting may be distinctive approach developing particular agents helpful treating OA. This review aims update most progress development by pathway. In this, we introduce pathways their crosstalk with other highlight role key regulator development. Several articles have reviewed Wnt from different aspects. candid provides an introduction development, highlighting latest research. Particularly, emphasise state-of-the-art promising therapeutic challenges nanocarrier-based delivery modulators

Language: Английский

Citations

4

Therapeutic Potential of Natural Compounds to Modulate WNT/β-Catenin Signaling in Cancer: Current State of Art and Challenges DOI Open Access
Anna Gajos-Michniewicz, Małgorzata Czyż

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12804 - 12804

Published: Nov. 28, 2024

Targeted therapies and immunotherapies have improved the clinical outcome of cancer patients; however, efficacy treatment remains frequently limited due to low predictability response development drug resistance. Therefore, novel therapeutic strategies for various types are needed. Current research emphasizes potential value targeting WNT/β-catenin dependent signaling that is deregulated in types. Targeting pathway with diverse synthetic natural agents subject a number preclinical studies trials patients. The usage nature-derived attributed their health benefits, reduced toxicity side effects compared agents. review summarizes ongoing aim target components across spectrum types, highlighting improve treatment.

Language: Английский

Citations

1

The Immune System’s Role in Glymphatic System Clearance and Alzheimer’s Disease Risk: A Csf Proteome-wide Study DOI Creative Commons
Natàlia Cullell, Giovanni Caruana,

Andrea Elias

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract Background: The emerging evidence of the role glymphatic system (GS) in Alzheimer’s disease (AD) provides new opportunities for intervention from earliest stages disease. aim study is to evaluate efficacy GS AD identify biomarkers. Methods: We performed a two-stage proteomic health using intravenous gadolinium-based contrast agent (GBCA) with serial T1 3T magnetic resonance imaging (MRI) individuals amnestic mild cognitive impairment (a-MCI). In Stage 1 (evaluated Cohort a-MCI participants (n=11)), we correlated levels 7K CSF proteins (estimated by SOMAscan) 78 Freesurfer-segmented brain regions interest (ROIs). Results: A total 173 different were significantly associated (p-value < 10-3). stronger correlations identified GRAAK, NSUN6, SHPS1 and TIM-4. pathway enrichment analysis revealed that mainly implicated neurodegenerative processes, immunity inflammation. 2, validated these results cohort (with without pathology (aMCI(-) aMCI(+); n = 22 7, respectively) healthy controls (n 10). Proteomic prediction models generated each ROI. These compared demographic-only identifying aMCI(-) aMCI(+) vs controls. This was repeated determine if could those both found outperform models. Conclusions: Our identifies linked involved immune participants.

Language: Английский

Citations

0

GPCR genes as a predictor of glioma severity and clinical outcome DOI Creative Commons

Eun‐A Ko,

Tong Zhou

Journal of International Medical Research, Journal Year: 2022, Volume and Issue: 50(7)

Published: July 1, 2022

To undertake a comprehensive analysis of the differential expression G protein-coupled receptor (GPCR) genes in order to construct GPCR gene signature for human glioma prognosis.This current study investigated several transcriptomic datasets and identified potentially associated with severity.A comprising 13 (nine upregulated four downregulated high-grade glioma) was developed. The predictive power 13-gene tested two validation cohorts strong positive correlation (Spearman's rank test: ρ = 0.649 Validation1 cohort; 0.693 Validation2 cohort) observed between grade based severity score both cohorts. also prognosis on Kaplan-Meier survival curve analyses Cox proportional hazard regression patients glioma.Knowledge may help researchers gain better understanding pathogenesis glioma. Further studies are needed validate association these pathogenesis.

Language: Английский

Citations

2

Estudo do receptor Frizzled classe 9 (FZD9), um potencial biomarcador prognóstico e alvo terapêutico DOI Creative Commons
Daniel Rodrigues de Bastos

Published: May 5, 2023

Citations

0

Exosomes promote axon outgrowth by engaging the Wnt-Planar Cell Polarity pathway DOI Open Access
Samar Ahmad,

Melanie Pye,

Masahiro Narimatsu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 28, 2023

In neurons, the acquisition of a polarized morphology is achieved upon outgrowth single axon from one several neurites. Exosomes or small extracellular vesicles (sEVs) diverse sources are known to promote neurite and thus may have therapeutic potential. However, effect fibroblast-derived exosomes on elongation in neurons central nervous system under growth permissive conditions remains unclear. Here, we show that sEVs neuronal mouse primary embryonic cortical neurons. Mechanistically, demonstrate sEV-induced increase requires endogenous Wnts core PCP components including Prickle, Vangl, Frizzled Dishevelled. We internalized by colocalize with Wnt7b induce relocalization Vangl2 distal during outgrowth. contrast, derived astrocytes do not outgrowth, while activated inhibit elongation. Thus, our data reveals through Wnt-PCP pathway manner dependent Wnts.

Language: Английский

Citations

0