bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 28, 2023
In
neurons,
the
acquisition
of
a
polarized
morphology
is
achieved
upon
outgrowth
single
axon
from
one
several
neurites.
Exosomes
or
small
extracellular
vesicles
(sEVs)
diverse
sources
are
known
to
promote
neurite
and
thus
may
have
therapeutic
potential.
However,
effect
fibroblast-derived
exosomes
on
elongation
in
neurons
central
nervous
system
under
growth
permissive
conditions
remains
unclear.
Here,
we
show
that
sEVs
neuronal
mouse
primary
embryonic
cortical
neurons.
Mechanistically,
demonstrate
sEV-induced
increase
requires
endogenous
Wnts
core
PCP
components
including
Prickle,
Vangl,
Frizzled
Dishevelled.
We
internalized
by
colocalize
with
Wnt7b
induce
relocalization
Vangl2
distal
during
outgrowth.
contrast,
derived
astrocytes
do
not
outgrowth,
while
activated
inhibit
elongation.
Thus,
our
data
reveals
through
Wnt-PCP
pathway
manner
dependent
Wnts.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research,
Journal Year:
2022,
Volume and Issue:
1869(10), P. 119305 - 119305
Published: June 8, 2022
The
Dishevelled
gene
was
first
identified
in
Drosophila
mutants
with
disoriented
hair
and
bristle
polarity
subsequent
work
has
now
demonstrated
its
importance
critical
diverse
aspects
of
biology.
Since
those
early
discoveries,
been
shown
to
coordinate
a
plethora
developmental
cellular
processes
that
range
from
controlling
cell
during
gastrulation
partnering
chromatin
modifying
enzymes
regulate
histone
methylation
at
genomic
loci.
While
the
role
DVL
development
is
well-respected
cytosolic
function
studied
more
extensively,
nuclear
continues
remain
murky.
In
this
review
we
highlight
some
seminal
discoveries
have
contributed
field,
but
primary
focus
discuss
recent
advances
respect
Dishevelled.
This
dimension
which
proving
be
increasingly
important
yet
remains
enigmatic.
Toxics,
Journal Year:
2022,
Volume and Issue:
10(6), P. 325 - 325
Published: June 14, 2022
Many
anthropogenic
pollutants
such
as
metals
are
discharged
into
the
marine
environment
through
modern
sources.
Among
these,
lithium
(Li),
nickel
(Ni),
and
zinc
(Zn)
can
interfere
with
biological
processes
in
many
organisms
when
their
concentration
rises.
These
toxic
to
sea
urchin
embryos,
affecting
development.
Indeed,
animal/vegetal
dorso/ventral
embryonic
axes
differently
perturbed:
Li
is
a
vegetalizing
agent,
Ni
disrupt
dorso-ventral
axis,
Zn
be
animalizing.
To
address
molecular
response
adopted
by
embryos
cope
these
or
involved
gene
networks
regulating
embryogenesis,
detect
new
biomarkers
for
evaluating
hazards
polluted
environments
well-known
vivo
model,
we
applied
high-throughput
screening
approach
embryos.
After
fertilization,
Paracentrotus
lividus
were
exposed
Li,
Ni,
24/48
h.
At
both
endpoints,
RNAs
analyzed
NanoString
nCounter
technology.
By
silico
analyses,
selected
panel
of
127
transcripts
encoding
regulatory
structural
proteins,
ranked
categories:
Apoptosis,
Defense,
Immune,
Nervous,
Development,
Biomineralization.
The
data
analysis
highlighted
dysregulation
genes
metal-dependent
manner.
A
functional
annotation
was
performed
KEEG
Orthology
database.
This
study
provides
platform
research
on
urchins.
Frontiers in Physiology,
Journal Year:
2023,
Volume and Issue:
14
Published: June 8, 2023
Disproportionate
to
the
severity
of
Alzheimer’s
disease
(AD)
and
huge
number
patients,
exact
treatment
prevention
AD
is
still
being
explored.
With
increasing
ageing,
search
for
means
prevent
treat
has
become
a
high
priority.
In
AD,
it
been
suggested
that
exercise
may
be
one
more
effective
less
costly
preventing
treating
therefore
large
part
current
research
aimed
at
exploring
effectiveness
in
AD.
However,
due
complexity
specific
pathogenesis
there
are
multiple
hypotheses
potential
mechanisms
interventions
need
This
review
specifically
summarises
interaction
between
from
molecular
perspective,
based
on
available
evidence
animal
models
or
human
experiments,
explores
them
categorised
according
pathologies
associated
with
AD:
can
activate
signalling
pathways
inhibited
by
(e.g.,
Wnt
PI3K/Akt
pathways)
reactivate
effects
downstream
factors
regulated
these
pathways,
thus
acting
alleviate
autophagic
dysfunction,
relieve
neuroinflammation
mitigate
Aβ
deposition.
addition,
this
paper
introduces
new
approach
regulate
blood-brain
barrier,
i.e.,
restore
stability
reduce
abnormal
phosphorylation
tau
proteins
neuronal
apoptosis.
concept.”
Motor
factors”
“Exerkines”,
which
act
through
autocrine,
paracrine
endocrine
stimulation
response
movement.
process,
we
believe
great
three
areas:
(1)
alleviation
movement
brain-gut
axis
(2)
combined
polyphenols
(3)
continued
exploration
movement-mediated
activation
pathway
Journal of drug targeting,
Journal Year:
2023,
Volume and Issue:
31(10), P. 1027 - 1049
Published: Nov. 16, 2023
Osteoarthritis
(OA)
is
a
highly
prevalent
chronic
joint
disease
and
the
leading
cause
of
disability.
Currently,
no
drugs
are
available
to
control
damage
or
ease
associated
pain.
The
wingless-type
(WNT)
signalling
pathway
vital
in
OA
progression.
Excessive
activation
WNT
pertinent
progression
severity.
Therefore,
agonists
antagonists
considered
potential
drug
candidates
for
treatment.
For
example,
SM04690,
novel
small
molecule
inhibitor
signalling,
has
demonstrated
its
recent
phase
III
clinical
trial
as
disease-modifying
osteoarthritis
(DMOAD).
targeting
may
be
distinctive
approach
developing
particular
agents
helpful
treating
OA.
This
review
aims
update
most
progress
development
by
pathway.
In
this,
we
introduce
pathways
their
crosstalk
with
other
highlight
role
key
regulator
development.
Several
articles
have
reviewed
Wnt
from
different
aspects.
candid
provides
an
introduction
development,
highlighting
latest
research.
Particularly,
emphasise
state-of-the-art
promising
therapeutic
challenges
nanocarrier-based
delivery
modulators
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(23), P. 12804 - 12804
Published: Nov. 28, 2024
Targeted
therapies
and
immunotherapies
have
improved
the
clinical
outcome
of
cancer
patients;
however,
efficacy
treatment
remains
frequently
limited
due
to
low
predictability
response
development
drug
resistance.
Therefore,
novel
therapeutic
strategies
for
various
types
are
needed.
Current
research
emphasizes
potential
value
targeting
WNT/β-catenin
dependent
signaling
that
is
deregulated
in
types.
Targeting
pathway
with
diverse
synthetic
natural
agents
subject
a
number
preclinical
studies
trials
patients.
The
usage
nature-derived
attributed
their
health
benefits,
reduced
toxicity
side
effects
compared
agents.
review
summarizes
ongoing
aim
target
components
across
spectrum
types,
highlighting
improve
treatment.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 18, 2024
Abstract
Background:
The
emerging
evidence
of
the
role
glymphatic
system
(GS)
in
Alzheimer’s
disease
(AD)
provides
new
opportunities
for
intervention
from
earliest
stages
disease.
aim
study
is
to
evaluate
efficacy
GS
AD
identify
biomarkers.
Methods:
We
performed
a
two-stage
proteomic
health
using
intravenous
gadolinium-based
contrast
agent
(GBCA)
with
serial
T1
3T
magnetic
resonance
imaging
(MRI)
individuals
amnestic
mild
cognitive
impairment
(a-MCI).
In
Stage
1
(evaluated
Cohort
a-MCI
participants
(n=11)),
we
correlated
levels
7K
CSF
proteins
(estimated
by
SOMAscan)
78
Freesurfer-segmented
brain
regions
interest
(ROIs).
Results:
A
total
173
different
were
significantly
associated
(p-value
<
10-3).
stronger
correlations
identified
GRAAK,
NSUN6,
SHPS1
and
TIM-4.
pathway
enrichment
analysis
revealed
that
mainly
implicated
neurodegenerative
processes,
immunity
inflammation.
2,
validated
these
results
cohort
(with
without
pathology
(aMCI(-)
aMCI(+);
n
=
22
7,
respectively)
healthy
controls
(n
10).
Proteomic
prediction
models
generated
each
ROI.
These
compared
demographic-only
identifying
aMCI(-)
aMCI(+)
vs
controls.
This
was
repeated
determine
if
could
those
both
found
outperform
models.
Conclusions:
Our
identifies
linked
involved
immune
participants.
Journal of International Medical Research,
Journal Year:
2022,
Volume and Issue:
50(7)
Published: July 1, 2022
To
undertake
a
comprehensive
analysis
of
the
differential
expression
G
protein-coupled
receptor
(GPCR)
genes
in
order
to
construct
GPCR
gene
signature
for
human
glioma
prognosis.This
current
study
investigated
several
transcriptomic
datasets
and
identified
potentially
associated
with
severity.A
comprising
13
(nine
upregulated
four
downregulated
high-grade
glioma)
was
developed.
The
predictive
power
13-gene
tested
two
validation
cohorts
strong
positive
correlation
(Spearman's
rank
test:
ρ
=
0.649
Validation1
cohort;
0.693
Validation2
cohort)
observed
between
grade
based
severity
score
both
cohorts.
also
prognosis
on
Kaplan-Meier
survival
curve
analyses
Cox
proportional
hazard
regression
patients
glioma.Knowledge
may
help
researchers
gain
better
understanding
pathogenesis
glioma.
Further
studies
are
needed
validate
association
these
pathogenesis.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 28, 2023
In
neurons,
the
acquisition
of
a
polarized
morphology
is
achieved
upon
outgrowth
single
axon
from
one
several
neurites.
Exosomes
or
small
extracellular
vesicles
(sEVs)
diverse
sources
are
known
to
promote
neurite
and
thus
may
have
therapeutic
potential.
However,
effect
fibroblast-derived
exosomes
on
elongation
in
neurons
central
nervous
system
under
growth
permissive
conditions
remains
unclear.
Here,
we
show
that
sEVs
neuronal
mouse
primary
embryonic
cortical
neurons.
Mechanistically,
demonstrate
sEV-induced
increase
requires
endogenous
Wnts
core
PCP
components
including
Prickle,
Vangl,
Frizzled
Dishevelled.
We
internalized
by
colocalize
with
Wnt7b
induce
relocalization
Vangl2
distal
during
outgrowth.
contrast,
derived
astrocytes
do
not
outgrowth,
while
activated
inhibit
elongation.
Thus,
our
data
reveals
through
Wnt-PCP
pathway
manner
dependent
Wnts.
