Disulfide-HMGB1 signals through TLR4 and TLR9 to induce inflammatory macrophages capable of innate-adaptive crosstalk in human liver transplantation

American Journal of Transplantation, Journal Year: 2023, Volume and Issue: 23(12), P. 1858 - 1871

Published: Aug. 9, 2023

Ischemia-reperfusion injury (IRI) during orthotopic liver transplantation (OLT) contributes to graft rejection and poor clinical outcomes. The disulfide form of high mobility group box 1 (diS-HMGB1), an intracellular protein released OLT-IRI, induces pro-inflammatory macrophages. How diS-HMGB1 differentiates human monocytes into macrophages capable activating adaptive immunity remains unknown. We investigated if binds toll-like receptor (TLR) 4 TLR9 differentiate that activate promote dysfunction. Assessment 106 tissue longitudinal blood samples revealed OLT recipients were more likely experience IRI dysfunction with increased reperfusion. Increased concentration also correlated TLR4/TLR9 activation, polarization macrophages, production anti-donor antibodies. In vitro, healthy volunteer stimulated purified had inflammatory cytokine secretion, antigen presentation machinery, reactive oxygen species production. TLR4 inhibition primarily impeded cytokine/chemokine costimulatory molecule programs, whereas decreased HLA-DR diS-HMGB1-polarized showed capacity present antigens T memory cells. murine OLT, treatment potentiated ischemia-reperfusion-mediated hepatocellular injury, accompanied by serum alanine transaminase levels. This translational study identifies the diS-HMGB1/TLR4/TLR9 axis as potential therapeutic targets in OLT-IRI recipients.

Language: Английский

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The dual role of toll-like receptors in COVID-19: Balancing protective immunity and immunopathogenesis

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137836 - 137836

Published: Nov. 1, 2024

Language: Английский

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Toll-like receptors in cardiac hypertrophy

Frontiers in Cardiovascular Medicine, Journal Year: 2023, Volume and Issue: 10

Published: April 11, 2023

Toll-like receptors (TLRs) are a family of pattern recognition (PRRs) that can identify pathogen-associated molecular patterns (PAMPs) and damage-associated (DAMPs). TLRs play an important role in the innate immune response, leading to acute chronic inflammation. Cardiac hypertrophy, cardiac remodeling phenotype during cardiovascular disease, contributes development heart failure. In previous decades, many studies have reported TLR-mediated inflammation was involved induction myocardium hypertrophic remodeling, suggesting targeting TLR signaling might be effective strategy against pathological hypertrophy. Thus, it is necessary study mechanisms underlying functions this review, we summarized key findings

Language: Английский

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Riding the wave of innovation: immunoinformatics in fish disease control

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e16419 - e16419

Published: Dec. 8, 2023

The spread of infectious illnesses has been a significant factor restricting aquaculture production. To maximise aquatic animal health, vaccination tactics are very successful and cost-efficient for protecting fish animals against many disease pathogens. However, due to the increasing number immunological cases their complexity, it is impossible manage, analyse, visualise, interpret such data without assistance advanced computational techniques. Hence, use immunoinformatics tools crucial, as they not only facilitate management massive amounts but also greatly contribute creation fresh hypotheses regarding immune responses. In recent years, advances in biotechnology have opened up new research avenues generating novel vaccines enhancing existing vaccinations outbreaks illnesses, thereby reducing losses. This review focuses on understanding silico epitope-based vaccine design, multi-epitope vaccines, molecular interaction immunogenic application based frequency reliable results. It believed that can bridge gap between experimental approaches reduce need research, so wet laboratory testing integrated with techniques may yield highly promising results be useful development fish.

Language: Английский

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Effects of aging and lifelong aerobic exercise on expression of innate immune components in skeletal muscle of women

Journal of Applied Physiology, Journal Year: 2024, Volume and Issue: 136(3), P. 482 - 491

Published: Jan. 11, 2024

This study examined the effects of aging and lifelong aerobic exercise on innate immune system components in skeletal muscle healthy women basal state after an unaccustomed resistance (RE) challenge. We also made exploratory between-sex comparisons with our previous report men. Three groups were studied: young exercisers (YE,

Language: Английский

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