Type 1 Diabetes Mellitus: A Review on Advances and Challenges in Creating Insulin Producing Devices

Micromachines, Journal Year: 2023, Volume and Issue: 14(1), P. 151 - 151

Published: Jan. 6, 2023

Type 1

Language: Английский

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Microfluidic systems for modeling digestive cancer: a review of recent progress

Biomedical Physics & Engineering Express, Journal Year: 2024, Volume and Issue: 10(5), P. 052002 - 052002

Published: Aug. 14, 2024

Abstract Purpose . This review aims to highlight current improvements in microfluidic devices designed for digestive cancer simulation. The emphasizes the use of multicellular 3D tissue engineering models understand complicated biology tumor microenvironment (TME) and progression. purpose is develop oncology research improve patients’ lives. Methods analyzes recent on mimicking cancer. It uses tissue-engineered devices, notably organs a chip (OOC), simulate human organ function lab. Cell cultivation modern three-dimensional hydrogel platforms allows precise geometry, biological components, physiological qualities. novel methodologies, key findings, technical progress explain this field’s advances. Results study discusses advances Micro systems with are emphasized. These capture complex biochemical gradients, niche variables, dynamic cell–cell interactions (TME). reveal stomach progression by duplicating TME. Recent discoveries technology have improved our understanding gut biology, as shown review. Conclusion Microfluidic play crucial role modeling furthering research. could transform drug development treatment revealing provides complete summary suggests future field professionals. review’s major goal further medical

Language: Английский

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Microfluidic-based human prostate-cancer-on-chip

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 23, 2024

Lack of adequate models significantly hinders advances in prostate cancer treatment, where resistance to androgen-deprivation therapies and bone metastasis remain as major challenges. Current vitro fail faithfully mimic the complex physiology. In vivo animal can shed light on oncogenes involved development progression; however, gland is fundamentally different from that human, underlying genetic mechanisms are different. To address this problem, we developed first microfluidic human Prostate-Cancer-on-Chip (PCoC) model, stromal fibroblast cells were co-cultivated two channels separated by a porous membrane under culture medium flow. The established microenvironment enables soluble signaling factors secreted each locally diffuse through pores affecting neighboring culture. We particularly explored conversion fibroblasts into cancer-associated (CAFs) due interaction between 2 cell types. Immunofluorescence microscopy revealed tumor induced CAF biomarkers, αSMA COL1A1, fibroblasts. level was observed increase along flow direction response diffusion agents, consistent with simulations solute concentration gradients. also downregulated androgen receptor (AR) expression fibroblasts, while an AR maintained normal homeostasis. further investigated invasion stroma, early step metastatic cascade, devices featuring serpentine channel orthogonal segments overlaying straight 8 µm-pore membrane. Both CAFs cross over their channel, stroma’s role seemed be proactive promoting invasion. As control, epithelial neither nor promoted summary, PCoC model allows spatiotemporal analysis tumor-stroma dynamic interactions, bi-directional physical contact, recapitulating tissue-level multicellular responses associated . Hence, it serve dissect seek advanced therapeutic strategies.

Language: Английский

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Advances, challenges and future applications of liver organoids in experimental regenerative medicine

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 24, 2025

The liver is a vital organ responsible for numerous metabolic processes in the human body, including metabolism of drugs and nutrients. After damage, can rapidly return to its original size if causative factor promptly eliminated. However, when harmful stimulus persists, liver’s regenerative capacity becomes compromised. Substantial theoretical feasibility has been demonstrated at levels gene expression, molecular interactions, intercellular dynamics, complemented by successful animal studies. robust model carrier that closely resemble physiology are still lacking translating these theories into practice. potential regeneration central focus ongoing research. Over past decade, advent organoid technology provided improved models materials advancing research efforts. Liver represents novel vitro culture system. several years refinement, organoids now accurately replicate morphological structure, nutrient drug metabolism, secretory functions, providing disease Regenerative medicine aims or tissue functions repair replace damaged tissues, restore their structure function, stimulate tissues organs within body. possess same function as tissue, offering serve viable replacement liver, aligning with goals medicine. This review examines role

Language: Английский

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Emerging microfluidics for the modeling and treatment of arthritis

Engineered Regeneration, Journal Year: 2024, Volume and Issue: 5(2), P. 153 - 169

Published: March 7, 2024

Microfluidic is a technology that allows the precise control of fluid in micro-channel. With its advantages high throughput and low cost, microfluidic has achieved good performance various fields recent years. Arthritis general term for variety joint diseases, which can be clinically manifested as pain swelling, seriously affecting people's physical mental health. At present, causes arthritis disease are still unknown, existing models treatment methods limited, so more treatments need to developed. organ chip cutting-edge build bionic human model, reflect structure function characteristics organs by simulating physiological environment tissues cells vitro. This paper reviews application modeling arthritis, hoping open up new vision study arthritis.

Language: Английский

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Microphysiological systems as models for immunologically ‘cold’ tumors

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: April 22, 2024

The tumor microenvironment (TME) is a diverse milieu of cells including cancerous and non-cancerous such as fibroblasts, pericytes, endothelial immune cells. intricate cellular interactions within the TME hold central role in shaping dynamics cancer progression, influencing pivotal aspects initiation, growth, invasion, response to therapeutic interventions, emergence drug resistance. In immunologically 'cold' tumors, marked by scarcity infiltrating cells, limited antigen presentation absence potent immune-stimulating signals, an abundance immunosuppressive factors. While strategies targeting avenue tumors have emerged, there pressing need for novel approaches that faithfully replicate complex non-cellular order develop targeted therapies can effectively stimulate responses improve outcomes patients. Microfluidic devices offer distinct advantages over traditional vitro 3D co-culture models vivo animal models, they better recapitulate key characteristics allow precise, controlled insights into dynamic interplay between various immune, stromal cell types at any timepoint. This review aims underscore microfluidic systems advancing our understanding presents current model aim dissect tumor-stromal, tumor-immune immune-stromal tumors. Understanding intricacies crucial devising effective reinvigorate overcome challenges immunotherapy approaches.

Language: Английский

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Prospects of emerging 3D bioprinting technologies: major technology components, technology developers, and end users—Part I

MGM Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 11(2), P. 331 - 339

Published: April 1, 2024

Abstract Bioprinting technology aims to create 3D structures with living cells mimic real tissue and organ functions. The process involves various additive technologies, including bioprinters bioinks. Bioinks comprise hydrogels, scaffolds, additives, growth factors, cells. While much of the is still in exploratory stages, it has successfully produced tissue, blood vessels, bones. Research suggests potential for bioprinting whole organs revolutionize medical procedures. Current major business components include types advanced Advancements technologies such as cellular reprogramming hold enhance development superior bioinks, thereby enabling fabrication bioprinted tissues. offers significant benefits across research, personalized medicine, other applications. This review provides a flavor using areas usage, Development (R&D), its applications on wide spectrum, medicine.

Language: Английский

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Advanced polymeric systems for colon drug delivery: from experimental models to market applications

Soft Matter, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In recent years, nano and micro drug delivery systems targeting the colon have gained more attention due to increasing interest in treating diseases such as colorectal cancer inflammatory bowel disease,

Language: Английский

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Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 13, 2025

Introduction Current in vitro intestinal models lack the mechanical forces present physiological environment, limiting their reliability for nanotoxicology studies. Here, we developed an enhanced Caco-2/HT29-MTX-E12 co-culture model incorporating orbital stimulation to better replicate conditions and investigate nanoparticle interactions. Methods We established co-cultures under static dynamic conditions, evaluating development through multiple approaches including barrier integrity measurements, gene expression analysis, confocal microscopy. introduced novel quantitative analysis of dome formation as a differentiation marker demonstrated application by investigating cellular responses titanium dioxide (TiO₂) nanoparticles digested food matrix. Results Dynamic accelerated epithelial differentiation, achieving functional properties day 14 rather than 21, with mucin production more organized three-dimensional structure. Mechanical selectively promoted goblet cell without affecting general markers. The optimized successfully detected concentration-dependent oxidative stress TiO₂ exposure, revealing dysfunction preceding membrane damage. Discussion This improved system provides platform By forces, each type exhibits representative behavior, creating realistic experimental setup. bridges gap between simple monocultures complex 3D systems, offering practical approach nanoparticle-epithelium interactions food-relevant context.

Language: Английский

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Harnessing CRISPR technology for next-generation microphysiological systems

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 185, P. 118164 - 118164

Published: Jan. 31, 2025

Language: Английский

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