Hypoxia-Inducible Factor and Oxidative Stress in Tendon Degeneration: A Molecular Perspective

Antioxidants, Journal Year: 2024, Volume and Issue: 13(1), P. 86 - 86

Published: Jan. 10, 2024

Tendinopathy is a debilitating condition marked by degenerative changes in the tendons. Its complex pathophysiology involves intrinsic, extrinsic, and physiological factors. While its intrinsic extrinsic factors have been extensively studied, role of factors, such as hypoxia oxidative stress, remains largely unexplored. This review article delves into contribution hypoxia-associated genes oxidative-stress-related to tendon degeneration, offering insights potential therapeutic strategies. The unique aspect this study lies pathway-based evidence, which sheds light on how these can be targeted enhance overall health.

Language: Английский

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Small organic molecules containing amorphous calcium phosphate: synthesis, characterization and transformation

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 12, 2024

As the primary solid phase, amorphous calcium phosphate (ACP) is a pivotal precursor in cellular biomineralization. The intrinsic interplay between ACP and Howard factor underscores significance of understanding their association for advancing biomimetic development. While organic compounds play established roles biomineralization, this study presents synthesis with naturally occurring (ascorbate, glutamate, itaconate) ubiquitously found mitochondria vital bone remodeling healing. developed was meticulously characterized using XRD, FTIR, solid-state 13 C 31 P NMR. morphological analysis revealed characteristic spherical morphology particle size close to 20 nm all synthesized variants. Notably, type compound strongly influences true density, specific surface area, size, transformation. vitro performed MC3T3-E1 cells, indicating highest cell viability ACP_ASC (ascorbate), followed by ACP_ITA (itaconate). lowest observed 10 %w/v ACP_GLU (glutamate); however, 1 cytocompatible. Further, effect small molecules on transformation low crystalline apatite (Ap) examined Milli-Q ® water, PBS, α-MEM.

Language: Английский

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The flexible chain: regulation of structure and activity of ETC complexes defines rate of ATP synthesis and sites of superoxide generation

Biophysical Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 25, 2025

Language: Английский

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Mitochondria-Rich Extracellular Vesicles from Bone Marrow Stem Cells Mitigate Muscle Degeneration in Rotator Cuff Tears in a Rat Model through Macrophage M2 Phenotype Conversion

Arthroscopy The Journal of Arthroscopic and Related Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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Engineered Mitochondrial Transplantation as An Anti-Aging Therapy

Aging and Disease, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Aging is an inevitable and complex biological process involving multi-factorial mechanisms. Mitochondrial dysfunction a critical factor in the aging process, characterized by decline mitochondrial quality activity, leading to aging-related diseases. Therefore, mitochondria have become attractive target anti-aging therapies. Several senolytic drugs targeting antioxidant agents been used research past few years. However, these strategies may cause adverse effects with long-term medication. In this extensive review, we propose "mitochondrial transplantation," which transfers healthy from donor cells recipient replace damaged or dysfunctional mitochondria, as new alternative strategy for treating aging-associated introduce contemporary landscape of transplantation, then discuss intensely successful applications transplantation therapy diseases such neurodegenerative diseases, cardiovascular aging, reproductive highlighting its translational potential. Finally, summarize prospect challenges opportunities faces therapy.

Language: Английский

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Physical Exercise and the Gut Microbiome: A Bidirectional Relationship Influencing Health and Performance

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3663 - 3663

Published: Oct. 28, 2024

Background/Objectives: The human gut microbiome is a complex ecosystem of microorganisms that can influence our health and exercise habits. On the other hand, physical also impact microbiome, affecting health. Our narrative review examines bidirectional relationship between activity as well potential for targeted probiotic regimens to enhance sports performance. Methods: We conducted comprehensive literature select articles published up till January 2024 on topics exercise, sports, probiotics, microbiota from major scientific databases, incorporating over 100 studies. Results: found varies with type intensity exercise. Moderate promotes healthy immune system, while high-intensity long duration cause leaky consequent systemic inflammation, which may disrupt microbial balance. Combining aerobic resistance training significantly affects bacterial diversity, linked lower prevalence chronic metabolic disorders. Furthermore, enhances increases SCFA production, improves nutrient utilization, modulates neural hormonal pathways, improving barrier integrity. findings showed supplementation associated decreased enhanced performance, fewer gastrointestinal disturbances, suggesting mutually influential. Conclusions: exemplified by how promote beneficial bacteria potentially ability through various mechanisms. These underscore importance adding tailored consider individual profiles into programs.

Language: Английский

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Ginsenoside Rc, an Active Component of Panax ginseng, Alleviates Oxidative Stress-Induced Muscle Atrophy via Improvement of Mitochondrial Biogenesis

Antioxidants, Journal Year: 2023, Volume and Issue: 12(8), P. 1576 - 1576

Published: Aug. 7, 2023

Loss of skeletal muscle mass and function has detrimental effects on quality life, morbidity, mortality, is particularly relevant in aging societies. The enhancement mitochondrial shown promise promoting differentiation function. Ginsenoside Rc (gRc), a major component ginseng, various pharmacological activities; however, its effect loss remains poorly explored. In this study, we examined the gRc hydrogen peroxide (H2O2)-induced reduction cell viability C2C12 myoblasts myotubes H2O2-induced myotube degradation. addition, investigated production intracellular reactive oxygen species (ROS) superoxide, ATP generation, peroxisome proliferator-activated receptor-gamma co-activator 1α (PGC-1α) activity under H2O2 treatment. Furthermore, to elucidate mechanism action gRc, conducted transcriptome analysis treated with or without effectively suppressed cytotoxicity, ROS, superoxide production, restored PGC-1α promoter activity, increased synthesis. Moreover, significantly affected expression levels genes involved maintaining biogenesis, while downregulating associated degradation oxidative stress. We provide compelling evidence supporting potential as promising treatment for weakness. Further investigations pathological conditions will contribute clinical development therapeutic intervention.

Language: Английский

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Mitochondrial Oxidative Stress and Mitophagy Activation Contribute to TNF-Dependent Impairment of Myogenesis

Antioxidants, Journal Year: 2023, Volume and Issue: 12(3), P. 602 - 602

Published: March 1, 2023

Many muscular pathologies are associated with oxidative stress and elevated levels of the tumor necrosis factor (TNF) that cause muscle protein catabolism impair myogenesis. Myogenesis defects caused by TNF mediated in part reactive oxygen species (ROS), including those produced mitochondria (mitoROS), but mechanism their pathological action is not fully understood. We hypothesized mitoROS act triggering enhancing mitophagy, an important tool for remodelling mitochondrial reticulum during used three recently developed probes-MitoTracker Orange CM-H2TMRos, mito-QC, MitoCLox-to study myogenesis human myoblasts. Induction resulted a significant increase generation phospholipid peroxidation inner membrane, as well mitophagy enhancement. Treatment myoblasts 24 h before induction decrease myoblast fusion index myosin heavy chain (MYH2) synthesis. increased mitoROS, membrane at early stage differentiation. Trolox SkQ1 antioxidants partially restored TNF-impaired The general autophagy inducers rapamycin AICAR, which also stimulate completely blocked suppression ULK1 inhibitor SBI-0206965 impaired TNF. Thus, mitoROS-dependent mitophagy.

Language: Английский

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Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Abstract Mitochondrial transfer emerges as a promising therapy for the restoration of mitochondrial function in damaged cells, mainly due to its limited immunogenicity. However, isolated mitochondria rapidly lose because they produce little energy outside cells. Therefore, this study investigates whether near infrared (NIR)‐mediated nicotinamide adenine dinucleotide (NADH) pre‐treatment enhances and stability mitochondria‐donor cells prior transplantation. Clinical applications NADH, an essential electron donor oxidative phosphorylation process, are restricted cellular uptake NADH. To address this, photo‐mediated method optimizes direct NADH delivery into increases absorption. L6 treated with irradiated NIR enhanced uptake, significantly improving production function. Importantly, improved functional characteristics maintained even after isolation from Primed mitochondria, i.e., those by NIR‐mediated (P‐MT), encapsulated fusogenic liposomes delivered muscle dysfunction. Compared conventional P‐MT promote greater recovery regeneration. These findings suggest that is effective strategy function, has potential lead novel treatments disorders degeneration.

Language: Английский

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Regulating Sirtuin 3-mediated mitochondrial dynamics through vanillic acid improves muscle atrophy in cancer-induced cachexia

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 9, 2025

Cancer cachexia is a cancer-associated disease characterized by gradual body weight loss due to pathologic muscle and fat loss, but effective treatments are still lacking. Here, we investigate the possible effect of vanillic acid (VA), known for its antioxidant, anti-inflammatory, anti-obesity effects, on mitochondria-mediated improvement cancer cachexia. We utilized cachexia-like models using CT26 colon dexamethasone. VA improved representative parameters including increased serum intereukin-6 levels. also attenuated in tibialis anterior gastrocnemius muscles, inhibited proteolytic markers RING-finger protein-1 (MURF1) atrophy F-box (MAFbx) mitochondrial function through alteration sirtuins 3 (SIRT3) mitofusin 1 (MFN1). Importantly, silencing SIRT3 gene abolished VA, indicating that important mechanism action VA. Overall, suggest as novel therapeutic agent can fundamentally treat recover patients.

Language: Английский

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