Chinese Medicine,
Journal Year:
2023,
Volume and Issue:
18(1)
Published: Sept. 12, 2023
Abstract
Background
Jian
Yun
Qing
Hua
Decoction
(JYQHD),
a
traditional
Chinese
medicine
decoction,
which
has
been
applied
in
the
treatment
of
gastric
cancer
(GC).
We
attempt
to
confirm
anti-gastric
effect
JYQHD
and
explore
mechanism
JYQHD.
Methods
Acute
toxicity
test
was
used
understand
studied
expression
prognostic
outcome
COL12A1
within
GC
tissues
through
network
databases.
Using
several
web-based
databases,
we
analyzed
major
components
targets
JYQHD,
as
well
known
therapeutic
cancer.
The
Venn
diagram
utilized
obtain
overlapped
genes.
Lentiviral
vector,
shRNAs
plasmids,
were
transfect
cells.
Cell
counting
kit-8
(CCK8),
sphere
formation,
malondialdehyde
(MDA),
glutathione
(GSH),
reactive
oxygen
species
(ROS),
Fe
2+
,
transmission
electron
microscopy
(TEM),
quantitative
Real-Time
Polymerase
Chain
Reaction
(qRT-PCR),
Western-Blot
(WB),
immunohistochemical
(IHC)
assays
employed
investigate
role
GC.
Results
results
showed
that
non-toxic
safe.
inhibited
growth
formation
ability
inducing
ferroptosis
cells,
suppressed
cells
induced
subcutaneous
xenograft
tumor
growth.
highly
expressed
tissues,
indicating
poor
prognosis.
specifically
enhanced
cell
progression
stemness
via
suppressing
ferroptosis.
down-regulated
order
suppress
Conclusion
development
by
inhibiting
pathway
mediated
COL12A1.
Korean Journal of Physiology and Pharmacology,
Journal Year:
2024,
Volume and Issue:
28(3), P. 183 - 196
Published: April 29, 2024
Ferroptosis
is
a
novel
mechanism
of
programmed
cell
death,
characterized
by
intracellular
iron
overload,
intensified
lipid
peroxidation,
and
abnormal
accumulation
reactive
oxygen
species,
which
ultimately
resulting
in
membrane
impairment
demise.Research
has
revealed
that
cancer
cells
exhibit
greater
demand
for
compared
to
normal
cells,
indicating
potential
susceptibility
ferroptosis.Stomach
colorectal
cancers
are
common
gastrointestinal
malignancies,
their
elevated
occurrence
mortality
rates
render
them
global
health
concern.Despite
significant
advancements
medical
treatments,
certain
unfavorable
consequences
drug
resistance
persist.Consequently,
directing
attention
towards
the
phenomenon
ferroptosis
gastric
holds
promise
enhancing
therapeutic
efficacy.This
review
aims
elucidate
intricate
cellular
metabolism
associated
with
ferroptosis,
encompassing
amino
acid
metabolism,
as
well
metabolic
processes.Furthermore,
significance
context
thoroughly
examined
discussed.
Journal of Translational Medicine,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Dec. 22, 2024
The
identification
of
ferroptosis
represents
a
pivotal
advancement
in
the
field
cell
death
research,
revealing
an
entirely
novel
mechanism
cellular
demise
and
offering
new
insights
into
initiation,
progression,
therapeutic
management
various
diseases.
Ferroptosis
is
predominantly
induced
by
intracellular
iron
accumulation,
lipid
peroxidation,
or
impairments
antioxidant
defense
system,
culminating
membrane
rupture
consequent
death.
Studies
have
associated
with
wide
range
diseases,
enhancing
our
comprehension
its
underlying
mechanisms,
we
can
formulate
innovative
strategies,
thereby
providing
renewed
hope
for
patients.
Frontiers in Genetics,
Journal Year:
2023,
Volume and Issue:
14
Published: April 18, 2023
Background:
Gastric
intestinal
metaplasia
(IM)
is
the
key
link
of
gastric
precancerous
lesions.
Ferroptosis
a
novel
form
programmed
cell
death.
However,
its
impact
on
IM
unclear.
The
focus
this
study
to
identify
and
verify
ferroptosis-related
genes
(FRGs)
that
may
be
involved
in
by
bioinformatics
analysis.
Materials
methods:
Differentially
expressed
(DEGs)
were
obtained
from
microarray
dataset
GSE60427
GSE78523
downloaded
Gene
Expression
Omnibus
(GEO)
database.
(DEFRGs)
overlapping
DEGs
FRGs
got
FerrDb.
DAVID
database
was
used
for
functional
enrichment
Protein-protein
interaction
(PPI)
analysis
Cytoscape
software
screen
hub
gene.
In
addition,
we
built
receiver
operating
characteristic
(ROC)
curve
verified
relative
mRNA
expression
quantitative
reverse
transcription-polymerase
chain
reaction
(qRT-PCR).
Finally,
CIBERSORT
algorithm
analyze
immune
infiltration
IM.
Results:
First,
total
17
DEFRGs
identified.
Second,
gene
module
identified
considered
as
gene:
PTGS2,
HMOX1,
IFNG,
NOS2.
Third,
ROC
showed
HMOX1
NOS2
had
good
diagnostic
characteristics.
qRT-PCR
experiments
confirmed
differential
normal
tissues.
immunoassay
proportion
T
cells
regulatory
(Tregs)
macrophages
M0
relatively
higher,
while
CD4
memory
activated
dendritic
lower.
Conclusion:
We
found
significant
associations
between
IM,
biomarkers
therapeutic
targets
These
results
enhance
our
understanding
contribute
treatment.
Human & Experimental Toxicology,
Journal Year:
2023,
Volume and Issue:
42
Published: March 1, 2023
Increasing
evidence
indicates
that
prolonged
exposure
to
sulforaphane
(SFN)
can
improve
malignancies.
However,
the
role
of
iron
in
SFN-triggered
death
gastric
carcinoma
cells
and
underlying
molecular
mechanisms
remain
unclear.
Thus,
current
study
explored
effects
SFN
on
overload-mediated
ferroptosis
PI3K/IRP2/DMT1
pathway
cells.We
utilized
MGC-803
cell
line
assess
whether
affected
metabolism
this
effect
contributed
death.
Pharmacological
inhibition
also
was
performed
determine
mechanism
overload
disturbance
metabolism.Our
data
revealed
treatment
altered
homeostasis
led
vitro.
Interestingly,
SFN-stimulated
resulted
from
ferroptosis,
a
recently
identified
iron-dependent
form
regulated
Furthermore,
an
chelator,
deferiprone,
ameliorated
mitochondrial
dysfunction
reduced
overload.
In
addition,
we
found
by
signaling
pathway.We
discovered
might
be
involved
cells.
Blockade
axis
could
provide
feedback
SFN-induced
protect
tumor
growth.
Chinese Medicine,
Journal Year:
2023,
Volume and Issue:
18(1)
Published: Sept. 12, 2023
Abstract
Background
Jian
Yun
Qing
Hua
Decoction
(JYQHD),
a
traditional
Chinese
medicine
decoction,
which
has
been
applied
in
the
treatment
of
gastric
cancer
(GC).
We
attempt
to
confirm
anti-gastric
effect
JYQHD
and
explore
mechanism
JYQHD.
Methods
Acute
toxicity
test
was
used
understand
studied
expression
prognostic
outcome
COL12A1
within
GC
tissues
through
network
databases.
Using
several
web-based
databases,
we
analyzed
major
components
targets
JYQHD,
as
well
known
therapeutic
cancer.
The
Venn
diagram
utilized
obtain
overlapped
genes.
Lentiviral
vector,
shRNAs
plasmids,
were
transfect
cells.
Cell
counting
kit-8
(CCK8),
sphere
formation,
malondialdehyde
(MDA),
glutathione
(GSH),
reactive
oxygen
species
(ROS),
Fe
2+
,
transmission
electron
microscopy
(TEM),
quantitative
Real-Time
Polymerase
Chain
Reaction
(qRT-PCR),
Western-Blot
(WB),
immunohistochemical
(IHC)
assays
employed
investigate
role
GC.
Results
results
showed
that
non-toxic
safe.
inhibited
growth
formation
ability
inducing
ferroptosis
cells,
suppressed
cells
induced
subcutaneous
xenograft
tumor
growth.
highly
expressed
tissues,
indicating
poor
prognosis.
specifically
enhanced
cell
progression
stemness
via
suppressing
ferroptosis.
down-regulated
order
suppress
Conclusion
development
by
inhibiting
pathway
mediated
COL12A1.