Quantitative proteomics unveils potential plasma biomarkers and provides insights into the pathophysiological mechanisms underlying equine metabolic syndrome DOI

Elisa María Espinosa-López,

Beatriz Ortiz-Guisado,

E. Díez de Castro

et al.

Published: April 7, 2025

Abstract Background Equine Metabolic Syndrome (EMS) is a multifactorial endocrine disorder characterized by obesity, insulin dysregulation (ID), and an increase in the risk of laminitis, painful condition that can lead to euthanasia severe cases. Diagnosing EMS challenging often relies on clinical history including difficulty losing weight, recurring episodes laminitis. The gold standard for laboratory support diagnosis identification ID, being basal simplest most accessible method. However, various factors such as diet, age, stress, season, testing protocols influence results. Dynamic tests like oral sugar test (OST) are preferred but present limitations due low sensitivity poor repeatability. These diagnostic challenges make difficult detect veterinary medicine highlighting need effective method early detection prevent laminitis its associated complications. Results Mass spectrometry-based proteomics represents powerful tool identify biomarkers explore molecular pathways related underlying pathology. In current study we established integrated pipeline plasma diagnosis. We compared proteomes from healthy horses, non-ID obese horses animals diagnosed with EMS. This comparison revealed 76 proteins significant changes (1% FDR) between groups. Conclusions Our demonstrates complement system, coagulation cascade extracellular matrix remodelling altered findings offer new insights into basis development led nomination several potential detection.

Language: Английский

Hepatokines: unveiling the molecular and cellular mechanisms connecting hepatic tissue to insulin resistance and inflammation DOI
Xiaolei Miao,

Arian Alidadipour,

Vian Saed

et al.

Acta Diabetologica, Journal Year: 2024, Volume and Issue: 61(11), P. 1339 - 1361

Published: July 20, 2024

Language: Английский

Citations

8
Fetuin-A: a relevant novel serum biomarker for non-invasive diagnosis of metabolic dysfunction-associated steatotic liver disease (MASLD): a retrospective case-control study DOI Creative Commons
Mohamed Mahmoud Elhoseeny,

Badawy A. Abdulaziz,

Mohamed A. Mohamed

et al.

BMC Gastroenterology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 18, 2024

Abstract Objectives To determine how fetuin-A contributes to diagnosing and assessing MASLD severity. Methods Fifty patients fifty healthy control participants were involved in this retrospective case-control research. Abdominal ultrasonography, fibroscan with controlled attenuated parameter scan (CAP scan), laboratory investigation (including assessment), clinical examination, history-taking performed on every case. Results Fetuin-A level was considerably higher the Cases group (1154.85 ± 629.89) than Control (505.29 150.4) ( p < 0.001). had significant validity prediction of at a cut-off > 702.5 82% sensitivity, 90% specificity, 86% overall accuracy. Conclusion One possible marker for diagnosis could be fetuin-A. Furthermore, substantial association between such severity disease as it revealed correlation ultrasound grading parameters. Trial registration 1- Pan African Clinical Registry. Unique Identifying number/registration ID: PACTR202309644280965. URL: https://pactr.samrc.ac.za/TrialDisplay.aspx?TrialID=26860 . Registration Approval date: 21/09/2023. 2- ClinicalTrials.gov number /registration NCT06097039. https://clinicaltrials.gov/study/NCT06097039?cond=NCT06097039&rank=1 25/10/2023.

Language: Английский

Citations

7
Candidate protein biomarkers in chronic kidney disease: a proteomics study DOI Creative Commons
Zhalaliddin Makhammajanov,

Assem Kabayeva,

Dana Auganova

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 18, 2024

Abstract Proteinuria poses a substantial risk for the progression of chronic kidney disease (CKD) and its related complications. Kidneys excrete hundreds individual proteins, some with potential impact on CKD or as marker disease. However, available data specific urinary proteins their relationship severity remain limited. Therefore, we aimed to investigate proteome association function in patients healthy controls. The proteomic analysis urine samples showed stage-specific differences number detected exponentially modified protein abundance index total (p = 0.007). Notably, such B2MG, FETUA, VTDB, AMBP exhibited robust negative associations compared Also, A1AG2, CD44, CD59, CERU, KNG1, LV39, OSTP, RNAS1, SH3L3, UROM positive entire cohort, while A1BG, CERU consistently displayed This study suggests that which were found be negatively positively associated patients, can serve markers dysfunctional functional kidneys, respectively.

Language: Английский

Citations

6
The Relationship of Fetuin-A with Coronary Calcification, Carotid Atherosclerosis, and Mortality Risk in Non-Dialysis Chronic Kidney Disease DOI Creative Commons
Osama Mohamed,

Mahmoud Ragab Mohamed,

Israa Gamal Hassan

et al.

Journal of Lipid and Atherosclerosis, Journal Year: 2024, Volume and Issue: 13(2), P. 194 - 194

Published: Jan. 1, 2024

This study investigated the relationship of fetuin-A with coronary calcification, carotid atherosclerosis, and mortality risk in non-dialysis chronic kidney disease (CKD).

Language: Английский

Citations

5
Influence of Adipokines on Metabolic Dysfunction and Aging DOI Creative Commons
Seongjoon Park, Isao Shimokawa

Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 873 - 873

Published: April 15, 2024

Currently, 30% of the global population is overweight or obese, with projections from World Obesity Federation suggesting that this figure will surpass 50% by 2035. Adipose tissue dysfunction, a primary characteristic obesity, closely associated an increased risk metabolic abnormalities, such as hypertension, hyperglycemia, and dyslipidemia, collectively termed syndrome. In particular, visceral fat accretion considered hallmark aging strongly linked to higher mortality rates in humans. Adipokines, bioactive peptides secreted adipose tissue, play crucial roles regulating appetite, satiety, adiposity, balance, thereby rendering them key players alleviating diseases potentially extending health span. review, we elucidated role adipokines development obesity related disorders while also exploring potential certain candidates for longevity interventions.

Language: Английский

Citations

4
Associations of urinary fetuin-A with histopathology and kidney events in biopsy-proven kidney disease DOI Creative Commons
Ming‐Tsun Tsai, Wei‐Cheng Tseng, Kuo‐Hua Lee

et al.

Clinical Kidney Journal, Journal Year: 2024, Volume and Issue: 17(4)

Published: March 14, 2024

Fetuin-A is implicated in the pathogenesis of vascular calcification chronic kidney disease (CKD); however, relationship between fetuin-A, histopathologic lesions and long-term outcomes patients with various types remains unclear. We measured urinary fetuin-A levels 335 individuals undergoing clinically indicated native biopsy. The expressions mRNA protein were assessed using RNA sequencing immunohistochemistry. association major adverse events (MAKE), defined as a decline estimated glomerular filtration rate (eGFR) at least 40%, failure or death, was analyzed. Urinary showed positive correlation albuminuria (rs = 0.67, P < .001) negative eGFR -0.46, .001). After multivariate adjustment, higher associated inflammation, mesangial expansion, interstitial fibrosis tubular atrophy, arteriolar sclerosis. Using 1 transcript per million gene expression cutoff, we found below threshold both normal function those CKD. Additionally, immunohistochemistry revealed reduced staining cells CKD compared controls. During median 21-month follow-up, 115 experienced MAKE, Cox regression analysis confirmed significant elevated MAKE. This remained after adjusting for potential confounding factors. histological damage clinical across spectrum biopsy-proven diseases.

Language: Английский

Citations

4
Novel serum biomarkers for early diagnosis of gestational diabetes mellitus—a review DOI Creative Commons
Michał Kunicki, Natalia Rzewuska,

Paulina Sopońska

et al.

Gynecological Endocrinology, Journal Year: 2025, Volume and Issue: 41(1)

Published: Jan. 21, 2025

Gestational diabetes mellitus (GDM) affects 9–25% of pregnancies. Undiagnosed or poorly managed GDM is associated with both short- and long-term complications in the fetus mother. The pathogenesis complex has not yet been fully elucidated. Several biomarkers found maternal serum have potential for early diagnosis GDM. aim this narrative review was to explore novel that comprehensively described previous reviews. We believe these may allow detection stages pregnancy, enabling timely proper treatment potentially preventing mother fetus.

Language: Английский

Citations

0
PTM-Fetuin-A: A Novel Biomarker for Early Detection of Diabetic Kidney Disease DOI Open Access
Marta Benková, Petar Petrov,

Petrov Aleksandar

et al.

Journal of Clinical Nephrology, Journal Year: 2025, Volume and Issue: 9(1), P. 013 - 019

Published: Jan. 24, 2025

Chronic Kidney Disease (CKD) is a significant public health issue with rising prevalence globally. Diabetic kidney disease (DKD), leading cause of CKD, necessitates improved biomarkers for early detection and effective management. Traditional markers such as serum creatinine, estimated glomerular filtration rate (eGFR), albuminuria have notable limitations in sensitivity specificity, especially detection. Fetuin-A, specifically its post-translationally modified form (PTM-Fetuin-A), has emerged potential novel biomarker DKD. This study evaluates PTM-Fetuin-A cohort Bulgarian patients type 1 2 diabetes, assessing correlation traditional eGFR. Significant correlations were observed between these indicators (e.g., Pearson’s r = 0.447, p 0.025 albuminuria), highlighting ability to detect function decline. Furthermore, demonstrated non-invasive tool identifying normoalbuminuric DKD, addressing gaps left by conventional biomarkers. By offering additional prognostic value, could improve the diagnosis clinical management diabetic patients, reducing burden CKD.

Language: Английский

Citations

0
Urinary Fetuin-A with Specific Post-Translational Modification in Type 1 Diabetes Patients with Normoalbuminuria and Preserved Kidney Function DOI Creative Commons
Sandra Božičević, Tomislav Bulum, Lea Duvnjak

et al.

Diagnostics, Journal Year: 2025, Volume and Issue: 15(4), P. 423 - 423

Published: Feb. 10, 2025

Background/Objectives: Post-translationally modified peptide fragments of fetuin-A (FetA) were identified as a potential biomarker diabetic kidney disease (DKD). An independent association between urinary FetA-derived levels (uPTM3-FetA) and DKD progression in patients with type 2 diabetes (T2D) was evidenced. This study aimed to explore uPTM3-FetA excretion its associations insulin resistance, inflammatory metabolic biomarkers 1 (T1D), the normal albuminuria estimated glomerular filtration rate (eGFR) > 60 mL/min/1.73 m2. Methods: aliquots 24 h urine specimens, routine laboratory renal, tests, adipokines (leptin, adiponectin, resistin), assessed glucose disposal (eGDR), measured cohort 169 adult T1D patients. To evaluate changes early renal dysfunction, divided according median eGFR. Above- below-median-eGFR groups considered having declining function, respectively. Results: The (IQR) level 11.7 (8.43–16.65 µg/24 h), no significant difference males females, well function However, sex-specific analysis revealed significantly higher male lower eGFRs, when compared those whereas such observed female BMI, hs-CRP, resistin HDL-cholesterol predictors excretion. Conclusions: Our results implicate role detection an dysfunction T1DM pinpoint importance approach diagnostics research.

Language: Английский

Citations

0
Biomarkers to predict or measure steroid resistance in idiopathic nephrotic syndrome: A systematic review DOI Creative Commons
Carl May, Nathan Ford, Gavin I. Welsh

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0312232 - e0312232

Published: Feb. 13, 2025

In this systematic review we have sought to summarise the current knowledge concerning biomarkers that can distinguish between steroid-resistant nephrotic syndrome and steroid-sensitive syndrome. Additionally, aim select best evidence-base should be prioritised for further research. Pub med web of science databases were searched using “steroid resistant AND biomarker”. Papers published 01/01/2012 10/05/2022 included. did not compare steroid sensitive syndrome, report sensitivity/specificity or area under curve reviews/letters excluded. The selected papers then assessed bias QUADAS-2 tool. source biomarker, cut off, sensitivity/specificity, sample size all extracted. Quality assessment was performed BIOCROSS 17 studies included, comprising 15 case-control 2 cross-sectional studies. Given rarity difficulty in recruiting large cohorts, accepted despite their limitations. We present a range candidate along with scores relating quality original publications risk inform future investigations. None stated whether authors blinded patient’s disease when assessing index test cohort. Highlighting key problem field needs addressed. These must now tested much larger sizes. Using new biobanks such as one built by NURTuRE-INS team will very helpful regard.

Language: Английский

Citations

0