Y chromosome shredding in Anopheles gambiae: insight into the cellular dynamics of a novel synthetic sex ratio distorter.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 10, 2024

Abstract Despite efforts to explore the genome of malaria vector Anopheles gambiae , Y chromosome this species remains enigmatic. The large number repetitive and heterochromatic DNA sequences makes exceptionally difficult fully assemble, hampering progress gene editing techniques functional studies for chromosome. In study, we made use a bioinformatic platform identify Y-specific that served as target site CRISPR/Cas9 system. activity Cas9 in reproductive organs males caused damage Y-bearing sperm without affecting their fertility, leading strong female bias progeny. Cytological investigation allowed us meiotic defects investigate selection new synthetic sex ratio distorter addition, alternative promoters enable specific tissues developmental stages male mosquitoes, enabling shed light on role gametogenesis. This work paves way further insight into poorly characterised . Moreover, strain have generated promises be valuable tool advancement field biology, with potential support genetic strategies aimed at controlling mosquitoes other pest species. Author summary Genetic elements known drive can manipulate offspring, favouring or sex. fascinating phenomenon has inspired development systems several organisms. where females harbour XX XY chromosomes respectively, X-chromosome ‘shredded’ during gametogenesis, production non-functional X-bearing sperm, while are left intact able fertilise eggs. These produce offspring extremely biased towards males, which used tools control harmful insect populations. our applied molecular strategy Our aim was cellular consequences shredding chromosome, impact meiosis selection, achieve offspring. outcome study enhances understanding biological mechanisms behind sex-ratio distorters could inform ratio. Additionally, present sexing mostly females, providing fundamental deadly vector.

Language: Английский

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Gamma-aminobutyric acid (GABA) in flower nectar and its possible physiological and behavioral effects on insect pollinators

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 64, P. 101206 - 101206

Published: May 20, 2024

Language: Английский

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Y chromosome shredding in Anopheles gambiae: Insight into the cellular dynamics of a novel synthetic sex ratio distorter

PLoS Genetics, Journal Year: 2024, Volume and Issue: 20(6), P. e1011303 - e1011303

Published: June 7, 2024

Despite efforts to explore the genome of malaria vector Anopheles gambiae , Y chromosome this species remains enigmatic. The large number repetitive and heterochromatic DNA sequences makes exceptionally difficult fully assemble, hampering progress gene editing techniques functional studies for chromosome. In study, we made use a bioinformatic platform identify Y-specific that served as target site CRISPR/Cas9 system. activity Cas9 in reproductive organs males caused damage Y-bearing sperm without affecting their fertility, leading strong female bias progeny. Cytological investigation allowed us meiotic defects investigate selection new synthetic sex ratio distorter addition, alternative promoters enable specific tissues developmental stages male mosquitoes, enabling shed light on role gametogenesis. This work paves way further insight into poorly characterised . Moreover, strain have generated promises be valuable tool advancement field biology, with potential support genetic strategies aimed at controlling mosquitoes other pest species.

Language: Английский

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Mosquitoes as a model for understanding the neural basis of natural behaviors

Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 87, P. 102897 - 102897

Published: July 12, 2024

Language: Английский

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MACSFeD—a database of mosquito acoustic communication and swarming features

Database, Journal Year: 2024, Volume and Issue: 2024

Published: Jan. 1, 2024

Acoustic communication plays an important role during the courtship of many mosquito species. Male mosquitoes show strong attraction to female wing beat frequencies, mediated via spectral matching between frequency and male ear mechanical tuning frequency. Such acoustic typically occurs within swarms, male-dominated aggregations with species-specific properties. Despite hundreds relevant publications being available, lack a central platform hosting all associated data hinders research efforts limits cross-species comparisons. Here, we introduce MACSFeD (Mosquito Communication Swarming Features Database), interactive for exploration our comprehensive database containing 251 unique reports focusing on different aspects communication, including hearing function, phonotaxis, as well swarming parameters. serves easily accessible, efficient, robust visualization tool research. We envision that further in-depth studies could arise following use this new platform. Database URL: https://minmatt.shinyapps.io/MACSFeD/.

Language: Английский

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Auditory System of Blood-Sucking Mosquitoes (Diptera, Culicidae)

Сенсорные системы, Journal Year: 2024, Volume and Issue: 38(3), P. 3 - 30

Published: Sept. 8, 2024

Language: Английский

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The Auditory System of Blood-Sucking Mosquitoes (Diptera, Culicidae)

Neuroscience and Behavioral Physiology, Journal Year: 2024, Volume and Issue: 54(9), P. 1447 - 1465

Published: Nov. 1, 2024

Language: Английский

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