Integrated Evaluation of Clinical Reproductive Potential Post Embryo Transfer Using Raman Metabolic Spectroscopy of Multistage Culture Medium DOI

Shouxin Zhang,

Guang-Zhong Jiao,

Bo Liang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract Background Utilizing Raman spectroscopy detection technique for assessing the clinical pregnancy outcomes following in vitro fertilization-embryo transfer (IVF-ET). Methods A total of 76 couples took part research. During their infertility treatment, culture medium samples were collected from embryos (40 and 36 non-pregnancy) at both D3 D5 stages. In total, 747 spectra recorded these samples. The statistical analysis results evaluated using accuracy, precision, recall, F1-score, Area Under Curve(AUC) to assess classification performance fusion model. biochemical involved comparing significant differences spectral signal intensities embryo metabolites corresponding different outcomes. Results This study established a model based on data predicting potential embryos. combination employing Hilbert-PCA, spectrogram concatenation fusion, SVM achieved an accuracy over 93%. Furthermore, indicated features obtained with varying potentials. Conclusions utilized non-invasive technology analyze metabolic across multiple developmental By establishing model, high-precision prediction was achieved. Trial registration: study, ethical review number 2021 − 384, approved by Institutional Review Board Yuhuangding Hospital, affiliated Qingdao University.

Language: Английский

Preimplantation genetic testing in the current era, a review DOI
Yafei Tian,

Mingan Li,

H. J. Yang

et al.

Archives of Gynecology and Obstetrics, Journal Year: 2024, Volume and Issue: 309(5), P. 1787 - 1799

Published: Feb. 20, 2024

Language: Английский

Citations

7
Viewing early life without labels: optical approaches for imaging the early embryo DOI Creative Commons
Darren J. X. Chow, Tiffany C. Y. Tan, Avinash Upadhya

et al.

Biology of Reproduction, Journal Year: 2024, Volume and Issue: 110(6), P. 1157 - 1174

Published: April 22, 2024

Abstract Embryo quality is an important determinant of successful implantation and a resultant live birth. Current clinical approaches for evaluating embryo rely on subjective morphology assessments or invasive biopsy genetic testing. However, both can be inherently inaccurate crucially, fail to improve the birth rate following transfer in vitro produced embryos. Optical imaging offers potential non-invasive accurate avenue assessing viability. Recent advances various label-free optical have garnered increased interest field reproductive biology due their ability rapidly capture images at high resolution, delivering morphological molecular information. This burgeoning holds immense further development, with profound implications translation. Here, our review aims to: (1) describe principles systems, distinguishing between that information, (2) highlight recent application these technologies biology, (3) assess respective merits limitations concerning capacity evaluate quality. Additionally, summarizes challenges translation systems into routine practice, providing recommendations future development. Finally, we identify suitable interrogating mechanisms underpinning

Language: Английский

Citations

4
Non-Male Factor Only—ICSI Can Overcome Oocyte Factor in PCOS Patients DOI Open Access

Yasmin Shibli Abu Raya,

Nardin Aslih, Yuval Atzmon

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(1), P. 244 - 244

Published: Jan. 3, 2025

Background: In this research, we retrospectively studied the influence of IVF vs. ICSI technique on embryo morphokinetics by means a time-lapse incubator in fresh cycles. Methods: A total 2645 treatment cycles resulting ovum pick-up 11,471 fertilized oocytes were included research from 2018 to 2022. The embryos grouped according or ICSI. Embryonic development was monitored using incubator, and they transferred day 3 5. Results: group developed faster had less fragmentation. However, fewer 2PNs achieved more discarded compared IVF. When sibling treated with either analyzed, found that resulted quicker higher KIDScores. Discussion: anovulation PCOS subgroups primary contributors high KIDScores oocytes, indicating might have beneficial effects oocyte factors, similar positive results it provides when male factors are involved. Conclusions: Women undergoing better used spontaneous This study reveals is superior female factor infertility.

Language: Английский

Citations

0
Non-invasive applications of Raman spectroscopy in assisted reproduction DOI Creative Commons
Mingxing Sui,

Lihui Si,

Zhuoyue Chen

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 16

Published: May 8, 2025

This review explores the non-invasive applications of Raman spectroscopy in assisted reproductive technology (ART). spectroscopy, a powerful tool for analyzing biological samples, has shown great potential enhancing ART outcomes through various such as sperm quality assessment, oocyte evaluation, and embryo selection. The non-destructive nature high specificity this technique enable detailed biochemical analysis at cellular level, offering valuable insights into processes without harming samples. highlights recent advancements studies demonstrating efficacy improving selection criteria gametes embryos, ultimately contributing to higher success rates ART. Future perspectives on integrating with other technologies further enhance its applicability medicine are also discussed.

Language: Английский

Citations

0
Noninvasive Biomarkers of Human Embryo Developmental Potential DOI Open Access
Jan Tesařík

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4928 - 4928

Published: May 21, 2025

There are two types of noninvasive biomarkers human embryo developmental potential: those based on a direct assessment morphology over time and using spent media after in vitro culture as source information. Both derived from previously acquired knowledge different aspects pre-implantation development. These include kinetics, chromosomal ploidy status, metabolism, embryonic gene transcription, translation, expression. As to the pertinent data can be obtained by analyzing sequential microscopic images cultured embryos. Spent serve genomic, metabolomic, transcriptomic proteomic markers. Methods used early pioneering studies, such microscopy, fluorescence situ hybridization, autoradiography, electrophoresis immunoblotting, or enzyme-linked immunosorbent assay, too subjective, invasive, and/or time-consuming. such, they unsuitable for current fertilization (IVF) practice, which needs objective, rapid, selection best uterine transfer cryopreservation. This has been made possible use high-throughput techniques time-lapse (for evaluation), next-generation sequencing, quantitative real-time polymerase chain reaction, high-performance liquid chromatography, nanoparticle tracking analysis, flow cytometry, mass spectroscopy, Raman near-infrared nuclear magnetic resonance spectroscopy analysis). In this review, individual markers presented systematically, with each marker’s history including available methodologies, strengths, limitations, so make essential information accessible all health professionals, even whose expertise matter is limited.

Language: Английский

Citations

0
Dynamic revelation of early development in mouse embryo via Raman spectroscopy DOI

Chenwei He,

Shan Huang, Runxin Yi

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126475 - 126475

Published: May 1, 2025

Language: Английский

Citations

0
The Role of One-Carbon Metabolism and Methyl Donors in Medically Assisted Reproduction: A Narrative Review of the Literature DOI Open Access
Konstantinos Sfakianoudis, Athanasios Zikopoulos, Sokratis Grigoriadis

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4977 - 4977

Published: May 2, 2024

One-carbon (1-C) metabolic deficiency impairs homeostasis, driving disease development, including infertility. It is of importance to summarize the current evidence regarding clinical utility 1-C metabolism-related biomolecules and methyl donors, namely, folate, betaine, choline, vitamin B12, homocysteine (Hcy), zinc, as potential biomarkers, dietary supplements, culture media supplements in context medically assisted reproduction (MAR). A narrative review literature was conducted PubMed/Medline database. Diet, ageing, endocrine milieu individuals affect both metabolism fertility status. In vitro fertilization (IVF) techniques, conditions particular, have a direct impact on activity gametes embryos. Critical analysis indicated that zinc supplementation cryopreservation may be promising approach reducing oxidative damage, while female serum levels employed possible biomarker for predicting IVF outcomes. Nonetheless, level low, future studies are needed verify these data. processes, redox defense epigenetic regulation, compromised IVF-derived The study lead way towards improving MAR efficiency safety ensuring lifelong health infants.

Language: Английский

Citations

2
Can time-lapse culture combined with artificial intelligence improve ongoing pregnancy rates in fresh transfer cycles of single cleavage stage embryos? DOI Creative Commons
Xiao Wang,

Qipeng Wei,

Weiyu Huang

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 29, 2024

Purpose With the rapid advancement of time-lapse culture and artificial intelligence (AI) technologies for embryo screening, pregnancy rates in assisted reproductive technology (ART) have significantly improved. However, clinical fresh cycles remain dependent on number type embryos transferred. The selection with highest implantation potential is critical embryologists influences transfer strategies fertility centers. superiority AI over traditional morphological scoring ranking cleavage-stage based their remains controversial. Methods This retrospective study analyzed 105 at Centre Reproductive Medicine from August 2023 to March 2024, following IVF/ICSI treatment cleavage stage. All were cultured using scored an automated model (iDAScore V2.0). Embryos categorized into three groups iDAScore V2.0: Group A (8 cells, iDA: 1.0-5.7); B 5.8-8.0); C (>8 5.8-8.0). Clinical outcomes, embryonic development, outcomes compared across groups. Results Baseline characteristics such as patient age, AMH levels, AFC, basal sex hormones showed no significant differences among (p > 0.05). iDAscores higher (7.3 ± 0.5) (6.7 (4.8 1.0) < 0.001). mean high-quality was (4.7 3.0), followed by (3.6 1.7) (2.1 1.2) There statistical difference = 0.392) ongoing rate single transfers between (54.5%, 30/55) (38.1%, 8/21), although there a tendency be higher. Conclusion Combining may enhance cycles.

Language: Английский

Citations

2
Raman spectroscopy: A promising analytical tool used in human reproductive medicine DOI

Chao Zheng,

Lumei Zhang,

Hefeng Huang

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2024, Volume and Issue: 249, P. 116366 - 116366

Published: July 15, 2024

Language: Английский

Citations

1
The synergy of morphokinetic parameters and sHLA-G in cleavage embryo enhancing implantation rates DOI Creative Commons

Chunyan Jiang,

Menghui Geng,

Can Zhang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: July 16, 2024

Objective: This study aimed to assess the relationship between implantation and soluble HLA-G (sHLA-G) expression in cleavage embryo culture medium (ECM) conjunction with early developmental kinetics determined by time-lapse imaging (TLI). Methods: A retrospective, single-center was conducted involving 238 embryos from 165 patients who underwent Frozen-thawed transfer (FET) using autologous oocytes, either single or double transfer. TLI morphokinetic parameters (t2, t3, t4, t5, t6, t7, t8, cc2, s2, cc3, s3) of were analyzed, sHLA-G levels D3 ECM measured an enzyme-linked immunosorbent assay (ELISA). hierarchical classification model developed categorize into five groups (A, B, C, D, E). The correlation levels, embryos, investigated establish a non-invasive method for evaluating potential. Multivariate logistic regression analysis performed identify potential influencing factors, receiver operating characteristic (ROC) curves used evaluate predictive value implantation. Results: unconditional indicated that t5 s3 level independent risk factors affecting rate decreased E. proposed effectively assessed embryos. higher positive group compared negative ( p < 0.001). ECM, combined model, accurately evaluated AUC 0.876. Conclusion: integration embryonic could reliably high likelihood successful

Language: Английский

Citations

1