Macrophage-mediated immunomodulation in biomaterial-assisted bone repair: Molecular insights and therapeutic prospects

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 488, P. 150631 - 150631

Published: March 26, 2024

The skeletal system is essential for preserving body structure and facilitating mobility; however, bone diseases frequently result in significant disabilities, necessitating the investigation into biomaterials restoration. Nevertheless, these may elicit immune reactions that obstruct healing process. Macrophages assume a pivotal role modulating inflammation sustaining homeostasis regeneration. A comprehensive understanding of molecular interactions between macrophages imperative development sophisticated designed to regulate environment promote remodeling. This review delves generation, polarization, interaction with principal cell types implicated osteogenesis, detailing mechanisms by which influence repair their response biomaterials. Additionally, it discusses strategies precise modulation macrophage functionality via intelligent mechanisms, outlines challenges constraints designing integrate functions. By elucidating challenges, lays groundwork creation advanced endowed smart immunomodulatory capabilities, heralding novel approaches treatment defects advancement regeneration therapies.

Language: Английский

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Interplay between mesenchymal stem cells and macrophages: Promoting bone tissue repair

World Journal of Stem Cells, Journal Year: 2024, Volume and Issue: 16(4), P. 375 - 388

Published: April 24, 2024

The repair of bone tissue damage is a complex process that well-orchestrated in time and space, focus difficulty orthopedic treatment. In recent years, the success mesenchymal stem cells (MSCs)-mediated clinical trials large-area defects necrosis has made it candidate engineering regenerative medicine. MSCs are closely related to macrophages. On one hand, regulate immune regulatory function by influencing macrophages proliferation, infiltration, phenotype polarization, while also affecting osteoclasts differentiation other activate mediate multilineage regulating microenvironment. cross-talk between plays crucial role system promoting regeneration. Making full use relationship will enhance efficacy therapy repair, provide reference for further application diseases.

Language: Английский

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C-C Motif Chemokine Ligand 2 Enhances Macrophage Chemotaxis, Osteogenesis, and Angiogenesis during the Inflammatory Phase of Bone Regeneration

Biomolecules, Journal Year: 2023, Volume and Issue: 13(11), P. 1665 - 1665

Published: Nov. 18, 2023

Local cell therapy has recently gained attention for the treatment of joint diseases and fractures. Mesenchymal stem cells (MSCs) are not only involved in osteogenesis angiogenesis, but they also have immunomodulatory functions, such as inducing macrophage migration during bone regeneration via crosstalk. C-C motif chemokine ligand 2 (CCL2), a known inflammatory mediator, is associated with macrophages inflammation. This study examined utility CCL2 therapeutic target local therapy. Using lentiviral vectors rabbit MSCs, genetically modified overexpressing MSCs were generated. Osteogenic differentiation assays performed using or without co-culture, performed. Additionally, co-cultures endothelial (ECs), angiogenesis was evaluated tube formation assay. Overexpression did affect under monoculture conditions promoted chemotaxis when co-cultured macrophages. Furthermore, CCL2-overexpression co-culture ECs. These results suggest that induces by promoting crosstalk between macrophages; stimulates ECs to induce angiogenesis. findings indicate may be useful areas loss.

Language: Английский

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LINC00330/CCL2 axis-mediated ESCC TAM reprogramming affects tumor progression

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: May 20, 2024

Abstract Background Tumor-associated macrophages (TAMs) significantly influence the progression, metastasis, and recurrence of esophageal squamous cell carcinoma (ESCC). The aberrant expression long noncoding RNAs (lncRNAs) in ESCC has been established, yet role lncRNAs TAM reprogramming during progression remains largely unexplored. Methods TAM-related were identified by intersecting differentially expressed with immune-related performing immune infiltration analysis. profile clinical relevance LINC00330 examined using TCGA database samples. overexpression interference sequences constructed to evaluate effect on progression. Single-cell sequencing data, CIBERSORTx, GEPIA utilized analyze within tumor microenvironment assess correlation between infiltration. ESCC-macrophage coculture experiments conducted investigate its subsequent interaction C–C motif ligand 2 (CCL2) was confirmed through transcriptomic sequencing, subcellular localization analysis, RNA pulldown, silver staining, immunoprecipitation, other experiments. Results is downregulated tissues strongly associated poor patient outcomes. Overexpression inhibits including proliferation, invasion, epithelial–mesenchymal transition, tumorigenicity vivo. promotes reprogramming, LINC00330-mediated binds CCL2 protein downstream signaling pathways. critical for Conclusions inhibited disrupting CCL2/CCR2 axis pathways an autocrine fashion; impeding CCL2-mediated a paracrine manner. new mechanism mediated LINC00330/CCL2 may provide potential strategies targeted immunocombination therapies patients ESCC. Graphical

Language: Английский

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Dual release scaffolds as a promising strategy for enhancing bone regeneration: an updated review

Nanomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18

Published: Jan. 31, 2025

Advancements in tissue regeneration, particularly bone regeneration is key area of research due to potential novel therapeutic approaches. Efforts reduce reliance on autologous and allogeneic grafts have led the development biomaterials that promote synchronized controlled healing. However, use growth factors limited by their short half-life, slow penetration, large molecular size toxicity. These suggest traditional delivery methods may be inadequate hence, address these challenges, new strategies are being explored. approaches include bioactive substances within advanced systems enable precise spatiotemporal control. Dual-release composite scaffolds offer a promising solution reducing need for multiple surgical interventions simplifying treatment process. allow sustained drug release, enhancing repair while minimizing drawbacks conventional methods. This review explores various dual-drug release systems, discussing modes action, types drugs used mechanisms improve regeneration.

Language: Английский

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Cranial repair-promoting effect of oxidised sodium alginate/amino gelatine injectable hydrogel loaded with deer antler blood peptides

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141116 - 141116

Published: Feb. 15, 2025

Language: Английский

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Efferocytosis and Bone Dynamics

Current Osteoporosis Reports, Journal Year: 2024, Volume and Issue: 22(5), P. 471 - 482

Published: June 24, 2024

Language: Английский

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Harnessing immunomodulation to combat sarcopenia: current insights and possible approaches

Immunity & Ageing, Journal Year: 2024, Volume and Issue: 21(1)

Published: Aug. 5, 2024

Sarcopenia is a complex age-associated syndrome of progressive loss muscle mass and strength. Although this condition influenced by many factors, age-related changes in immune function including cell dynamics, chronic inflammation contribute to its progression. The interplay between the system, gut-muscle axis, autophagy further underscores their important roles sarcopenia pathogenesis. Immunomodulation has emerged as promising strategy counteract sarcopenia. Traditional management approaches treat physical exercise nutritional supplementation, emerging technologies biophysical stimulation demonstrated importance immunomodulation regulation macrophages T cells reduction inflammation. Treatments alleviate low-grade older adults modulating gut microbial composition diversity combat Furthermore, some pharmacological interventions, nano-medicine, therapies targeting muscle, microbiota, or present additional avenues for This narrative review explores immunological underpinnings sarcopenia, elucidating relationship system during ageing. Additionally, discusses new areas such axis autophagy, which bridge health. Insights into current potential through modulation are provided, along with suggestions future research directions therapeutic strategies. We aim guide investigation clinical biomarkers identify indicators diagnosis treatment targets condition. also draw attention considering

Language: Английский

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Revolutionizing bone defect healing: the power of mesenchymal stem cells as seeds

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 21, 2024

Bone defects can arise from trauma or pathological factors, resulting in compromised bone integrity and the loss absence of tissue. As we are all aware, repairing is a core problem tissue engineering. While minor self-repair if periosteum remains intact normal osteogenesis occurs, significant conditions such as congenital imperfecta present substantial challenges to self-healing. research on mesenchymal stem cell (MSC) advances, new fields application have emerged; however, their orthopedics one most established clinically valuable directions. This review aims provide comprehensive overview progress regarding MSCs treatment diverse defects. MSCs, multipotent cells, offer advantages due immunomodulatory properties ability undergo osteogenic differentiation. The will encompass characteristics within microenvironment summarize different types defects, ranging fundamental animal studies clinical applications.

Language: Английский

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T cells and macrophages jointly modulate osteogenesis of mesenchymal stromal cells

Journal of Biomedical Materials Research Part A, Journal Year: 2024, Volume and Issue: 112(12), P. 2202 - 2209

Published: July 4, 2024

Approximately 5%-10% of fractures go on to delayed healing and nonunion, posing significant clinical, economic, social challenges. Current treatment methods involving open bone harvesting grafting are associated with considerable pain potential morbidity at the donor site. Hence, there is growing interest in minimally invasive approaches such as marrow aspirate concentrate (BMAC), which contains mesenchymal stromal cells (MSCs), macrophages (Mφ), T cells. However, use cultured or activated for not yet FDA-approved United States, necessitating further exploration optimal cell types proportions effective formation. As our understanding osteoimmunology advances, it has become apparent that factors from anti-inflammatory Mφ (M2) promote formation by MSCs. Additionally, M2 helper 2 (Th2) Treg cells, both enhance In this study, we investigated interactions among MSCs, Mφ, explored subsets BMAC. Coculture experiments were conducted using primary CD4+ specific ratios. Our results indicate nonactivated had no direct influence osteogenesis while coculturing MSCs a ratio 1:5:10 positively impacted Furthermore, higher numbers led increased polarization proportion Th2 early stages coculture. These findings suggest enhancing adjusting immune ratios By effects formation, can develop more strategies protocols treating defects nonunions. Further studies needed investigate these vivo explore additional influencing MSC-based therapies.

Language: Английский

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