Transcriptomic profiles of monocyte-derived macrophages exposed to SARS-CoV-2 VOCs reveal immune-evasion escape driven by delta

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 4, 2025

Since the breakout of COVID-19, mutated forms severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have shown enhanced rates transmission and adaptation to humans. The variants concern (VOC), designated Alpha, Beta, Gamma, Delta, Omicron emerged independent one another, in turn rapidly became dominant. success each VOC, as well virus fitness, were enabled by altered intrinsic functional properties and, reasonably, antigenicity changes, conferring ability evade a primed immune response. We analysed gene expression profiles monocyte-derived macrophages (MDM) isolated from whole blood healthy participants exposed 5 different SARS-CoV-2 VOC: D614G, Alpha (B.1.1.7), Gamma (P1), Delta (B.1.617.2), BA.1 (B.1.1.529), HCoV-OC43 strain, already present population before pandemic. Whole transcriptome RNA-Seq, for both coding non-coding RNAs, was then made. After exposure VOC MDM, we initially assessed presence viral transcripts confirm entry. RNA-Seq data observed significant deregulation RNAs. In particular, our analysis showed up-regulation several genes involved immunological processes, such PARP9/PARP14 axes, variants. Surprisingly, that variant exhibited transcriptional profile more similar naïve control group, while intermediate differentially expressed (DEGs) between two groups. By checking canonical markers M1/M2 differentiation states, did not observe any variant, suggesting an M0 status, comparable group. Finally, 3 main types RNAs (ncRNAs): long (lncRNAs), microRNAs (miRNAs), small nucleolar (snoRNAs), some which are common coronaviruses, specific SARS-CoV-2. SARS-CoV-2-dependent alteration macrophage (MDM)-infected cells can be linked chronological order variants' appearance human population. Our suggest evolution modulating host response, with strong change pace beginning advent variant. MDMs failure activation adaptive this correlates symptoms developed people affected

Language: Английский

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Long COVID: Molecular Mechanisms and Detection Techniques

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 408 - 408

Published: Dec. 28, 2023

Long COVID, also known as post-acute sequelae of SARS-CoV-2 infection (PASC), has emerged a significant health concern following the COVID-19 pandemic. Molecular mechanisms underlying occurrence and progression long COVID include viral persistence, immune dysregulation, endothelial dysfunction, neurological involvement, highlight need for further research to develop targeted therapies this condition. While clearer picture clinical symptomatology is shaping, many molecular are yet be unraveled, given their complexity high level interaction with other metabolic pathways. This review summarizes some most important symptoms associated that occur in well relevant techniques can used understanding pathogen, its affinity towards host, possible outcomes host-pathogen interaction.

Language: Английский

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Toward a Categorization of Virus-ncRNA Interactions in the World of RNA to Disentangle the Tiny Secrets of Dengue Virus

Viruses, Journal Year: 2024, Volume and Issue: 16(5), P. 804 - 804

Published: May 18, 2024

In recent years, the function of noncoding RNAs (ncRNAs) as regulatory molecules cell physiology has begun to be better understood. Advances in viral molecular biology have shown that host ncRNAs, cellular factors, and virus-derived ncRNAs their interplay are strongly disturbed during infections. Nevertheless, folding RNA virus genomes also been identified a critical factor regulating canonical non-canonical functions. Due influence structure genomes, complex processes infections modulated. We propose three main categories organize current information about RNA–RNA interactions some well-known human viruses. The first category shows examples associated with immune response triggered Even though miRNAs introduce standpoint, they briefly presented keep researchers moving forward uncovering other RNAs. second outlines between virus-host while third describes how genome serves scaffold for processing Our grouping may provide comprehensive framework classify ncRNA–host-cell emerging viruses diseases. this sense, we introduced them DENV–host-cell interactions.

Language: Английский

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The relationship between microRNAs and COVID-19 complications

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 10, P. 16 - 24

Published: Aug. 23, 2024

Language: Английский

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Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine

Diagnostics, Journal Year: 2025, Volume and Issue: 15(2), P. 229 - 229

Published: Jan. 20, 2025

COVID-19 has caused widespread morbidity and mortality, with its effects extending to multiple organ systems. Despite known risk factors for severe disease, including advanced age underlying comorbidities, patient outcomes can vary significantly. This variability complicates efforts predict disease progression tailor treatment strategies. While diagnostic therapeutic approaches are still under debate, RNA sequencing (RNAseq) emerged as a promising tool provide deeper insights into the pathophysiology of guide personalized treatment. A comprehensive literature review was conducted using PubMed, Scopus, Web Science, Google Scholar. We employed Medical Subject Headings (MeSH) terms relevant keywords identify studies that explored role RNAseq in diagnostics, prognostics, therapeutics. proven instrumental identifying molecular biomarkers associated severity patients COVID-19. It allows differentiation between asymptomatic symptomatic individuals sheds light on immune response mechanisms contribute progression. In critically ill patients, been crucial key genes may outcomes, guiding decisions, assessing long-term virus. Additionally, helped understanding persistence viral after recovery, offering new management post-acute sequelae, long COVID. significantly improves management, particularly by enhancing accuracy, personalizing treatment, predicting responses. refines stratification, improving holds promise targeted interventions both acute

Language: Английский

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Antiviral Therapy-Induced Changes in Long Non-Coding RNA Expression Profiles in Umbilical Cord Blood and Placental Tissues of Hepatitis B Virus-Infected Pregnant Women

International Journal of Women s Health, Journal Year: 2025, Volume and Issue: Volume 17, P. 835 - 844

Published: March 1, 2025

Hepatitis B virus (HBV) is a major global health concern, with maternal-fetal transmission being the primary route of transmission, which can lead to chronic HBV infection in newborns. Long non-coding RNAs (lncRNAs) play crucial roles gene regulation and immune responses, but their involvement during pregnancy remains unclear. This study aimed assess impact tenofovir disoproxil fumarate (TDF)-based antiviral therapy on lncRNA expression profiles signaling pathways umbilical cord blood placental tissues identify potential therapeutic targets for preventing intrauterine infection. Umbilical serum were collected from six carriers. Three carriers received TDF-based therapy, remaining who did not receive served as controls. LncRNA microarray analysis bioinformatics used evaluate effects pathways. Antiviral exerted minimal blood. In tissues, significant alterations observed, including 249 upregulated 381 downregulated lncRNAs. activated innate pathways, such intracellular DNA sensing, chemokine signaling, type I interferon, Jak-Stat, interferon-γ-mediated adaptive immunity. Through intersection analysis, CPED1 was found differentially expressed both tissues. KEGG pathway suggested that low may inhibit via JAK-STAT pathway. demonstrated altered offering insights into molecular mechanisms transmission.

Language: Английский

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Regulatory Non-Coding RNAs during Porcine Viral Infections: Potential Targets for Antiviral Therapy

Viruses, Journal Year: 2024, Volume and Issue: 16(1), P. 118 - 118

Published: Jan. 13, 2024

Pigs play important roles in agriculture and bio-medicine; however, porcine viral infections have caused huge losses to the pig industry severely affected animal welfare social public safety. During infections, many non-coding RNAs are induced or repressed by viruses regulate infection. Many have, therefore, developed a number of mechanisms that use ncRNAs evade host immune system. Understanding how immunity during is critical for development antiviral therapies. In this review, we provide summary classification, production function involved regulating infections. Additionally, outline pathways modes action which highlight therapeutic potential artificial microRNA. Our hope information will aid therapies based on industry.

Language: Английский

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Peripheral lncRNA NEAT-1, miR374b-5p, and IL6 panel to guide in COVID-19 patients’ diagnosis and prognosis

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313042 - e0313042

Published: Dec. 27, 2024

Background The SARS-CoV-2 virus’s frequent mutations have made disease control with vaccines and antiviral drugs difficult; as a result, there is need for more effective coronavirus drugs. Therefore, detecting the expression of various diagnostic biomarkers, including ncRNA in SARS-CoV2, implies new therapeutic strategies disease. Aim Our study aimed to measure NEAT-1, miR-374b-5p, IL6 serum COVID-19 patients, demonstrating correlation between target genes explore possible relationship them. Also, association patients’ clinical findings radiological severity indices will be explored. Patients methods current included 48 COVID-19-infected individuals 40 controls. Quantitative real-time PCR (qPCR) was performed detect lncRNA NEAT-1 miRNA374b-5p fold change (FC) participants’ sera. Enzyme-Linked Immune Sorbent Assay (ELISA) used . Results results showed statistical significance lower levels ( ) [ median (range) = 0.08 (0.001-0.602)], miR374b-5p 0.14 (.01-7.16)] while higher IL-6 41.3 (7.2-654) pg/ml] when compared controls p-value <0.001. Serum level correlates negatively r -.317, P .008). ROC curve analysis revealed that sensitivity specificity tests diagnosis cases illustrated (100% 97.9%) (85% 100%) at cut-off values (0.985 12.55), respectively. In comparison, around 85% distinguishing patients from No significant detected indices. Conclusions first decreased serum. There also an increase levels. negative patients.

Language: Английский

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A piRNA Portrait for Blood Cells of Healthy Donors and COVID-19 Patients

Published: Feb. 6, 2024

The expression of non-coding RNAs varies greatly depending on the cell type and physiological state. Changes in affect regulatory properties these molecules. We investigated RNA-seq data from sorted blood cells healthy control donors severe COVID-19 patients. Our aim was to display a distinguishable piRNA differential portrait for each study behavior Overall (erythrocytes, monocytes, lymphocytes, eosinophils, basophils, neutrophils) differs type. Four most abundant were expressed relatively equally all types, they included piR-49145, piR-49144, piR-49143 piR-33151. By reviewing group patients we observed 3 upregulated 10 downregulated piRNAs erythrocytes. Monocytes presented with larger amount statistically significant piRNA, 4 35 downregulated. In 19 upregulated.

Language: Английский

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Increased expression of miR-320b in blood plasma of patients in response to SARS-CoV-2 infection

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 13, 2024

Abstract Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2). Recent research has demonstrated how epigenetic mechanisms regulate the host–virus interactions in COVID-19. It also shown that microRNAs (miRNAs) are one of three fundamental regulation gene expression and play an important role viral infections. A pilot study published our group identified, through next-generation sequencing (NGS), miR-4433b-5p, miR-320b, miR-16–2-3p differentially expressed between patients with COVID-19 controls. Thus, objectives this were to validate these miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR) perform silico analyses. Patients (n = 90) healthy volunteers 40) recruited. MiRNAs extracted from plasma samples validated qRT-PCR. In addition, analyses performed mirPath v.3 software. MiR-320b was only miRNA upregulated case com-pared control group. The indicated miR-320b KITLG consequently inflammatory process. This confirmed can distinguish participants; however, further needed determine whether be used as a target or biomarker.

Language: Английский

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