Medicine International,
Journal Year:
2024,
Volume and Issue:
5(2)
Published: Dec. 20, 2024
The
limitations
of
two‑dimensional
(2D)
models
in
cancer
research
have
hindered
progress
fully
understanding
the
complexities
drug
resistance
and
therapeutic
failures.
However,
three‑dimensional
(3D)
provide
a
more
accurate
representation
in
vivo
environments,
capturing
critical
cellular
interactions
dynamics
that
are
essential
evaluating
efficacy
toxicity
tyrosine
kinase
inhibitors
(TKIs).
These
advanced
enable
researchers
to
explore
mechanisms
with
greater
precision,
optimizing
treatment
strategies
improving
predictive
accuracy
clinical
outcomes.
By
leveraging
3D
models,
it
will
be
possible
deepen
current
TKIs
drive
forward
innovations
treatment.
present
review
discusses
2D
transformative
impact
on
oncology
research,
highlighting
their
roles
addressing
challenges
systems
advancing
TKI
studies.
One Health Advances,
Journal Year:
2024,
Volume and Issue:
2(1)
Published: Aug. 2, 2024
Abstract
The
respiratory
tract
and
digestive
serve
as
the
gateway
between
host
environment,
playing
an
important
role
in
protecting
against
viral
infections.
Diseases
caused
by
viruses
that
infiltrate
gastrointestinal
tracts
account
for
major
infectious
diseases
pigs,
resulting
significant
economic
losses
swine
industry.
However,
studies
on
virus-host
interactions
are
limited
due
to
lack
of
suitable
research
models
can
effectively
stimulate
highly
complex
physiological
characteristics
found
vivo
.
With
advancement
stem
cell
technology,
organoids
more
closely
recapitulate
structure,
function,
organization
specific
organs
or
tissues
vitro
have
gradually
become
a
hotspot.
These
novel
ex
critical
studying
infection,
investigating
pathogenesis,
elucidating
developing
preventive
therapeutic
approaches.
Currently,
intestinal
(IOs)
been
widely
applied
study
diseases.
Therefore,
this
review
primarily
summarizes
development
porcine
organoids,
their
applications
current
limitations,
future
perspectives.
Immuno,
Journal Year:
2024,
Volume and Issue:
4(4), P. 312 - 324
Published: Sept. 27, 2024
Recent
advances
in
organoid
technology
have
revolutionized
cancer
biology
and
therapeutic
interventions,
offering
personalized
immunotherapy
treatment.
Organoids,
three-dimensional
cell
cultures
derived
from
patient
tumors,
accurately
replicate
the
tumor
microenvironment,
providing
unprecedented
insights
into
tumor-immune
interactions
responses.
In
this
literature-based
study,
we
discuss
various
culture
methods
for
diverse
applications
of
organoids
immunotherapy,
including
drug
screening,
treatment
strategies,
mechanistic
studies.
Additionally,
address
technological
challenges
associated
with
these
propose
potential
future
solutions
to
accelerate
development
novel
immunotherapeutic
approaches.
This
review
highlights
transformative
models
advancing
preclinical
modeling,
evaluation,
paving
way
more
effective
treatments.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 4, 2024
SUMMARY
Patient-derived
tumor
organoids
have
been
leveraged
for
disease
modeling
and
preclinical
studies,
but
rarely
applied
in
real-time
to
aid
with
interpretation
of
patient
treatment
responses
clinics.
We
recently
demonstrated
early
efficacy
signals
a
first-in-human,
phase
1
study
dual-targeting
chimeric
antigen
receptor
T
cells
(EGFR-IL13Rα2
CAR-T
cells)
patients
recurrent
glioblastoma.
Here
we
analyzed
six
sets
patient-derived
glioblastoma
(GBOs)
treated
concurrently
the
same
autologous
cell
products
as
our
study.
found
that
led
target
reduction
cytolysis
GBOs,
degree
which
correlated
engraftment
detected
patients’
cerebrospinal
fluid
(CSF).
Furthermore,
cytokine
release
patterns
GBOs
mirrored
CSF
samples
over
time.
Our
findings
highlight
unique
trial
design
valuable
platform
assessment
bioactivity
insights
into
immunotherapy
efficacy.
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: Nov. 26, 2024
Despite
extensive
efforts
to
unravel
tumor
behavior
and
develop
anticancer
therapies,
most
treatments
fail
when
advanced
clinical
trials.
The
main
challenge
in
cancer
research
has
been
the
absence
of
predictive
models,
accurately
mimicking
tumoral
processes
response
treatments.
microenvironment
(TME)
shows
several
human-specific
physical
chemical
properties,
which
cannot
be
fully
recapitulated
by
conventional
2D
cell
cultures
or
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 12, 2024
Abstract
Dogs
share
many
anatomical
and
physiological
characteristics
with
humans
and,
in
certain
instances,
represent
a
more
reliable
research
model
than
rodents
for
some
diseases,
including
several
types
of
human
cancer.
Advances
adult
stem
cell
have
accelerated
the
development
3D
organoid
cultures
non-model
animals,
such
as
dogs.
These
advancements
potential
to
significantly
impact
disease
modeling,
biomarker
discovery,
drug
both
canine
bladder
cancer
(BC).
In
this
Chapter,
we
present
standardized
protocol
generating
BC
organoids
from
canines.
The
chapter
describes
detailed
methodology
establishing
urine
or
tissue-derived
cells.
Additionally,
it
highlights
these
recapitulate
expression
key
markers
their
original
tissues
utility
various
biomedical
applications
screening,
gene
mutation
analysis,
toxicology.
Finally,
discuss
future
directions
translational
research.
Recent
advances
in
organoid
technology
have
revolutionized
cancer
biology
and
thera-peutic
interventions,
offering
personalized
immunotherapy
treatment.
Organoids,
three-dimensional
cell
cultures
derived
from
patient
tumors,
accurately
replicate
the
tumor
microenvi-ronment,
providing
unprecedented
insights
into
tumor-immune
interactions
therapeutic
re-sponses.
In
this
literature-based
study,
we
discuss
various
culture
methods
for
diverse
appli-cations
of
organoids
immunotherapy,
including
drug
screening,
treatment
strategies,
mechanistic
studies.
Additionally,
address
technological
challenges
asso-ciated
with
these
propose
potential
future
solutions
to
accelerate
development
novel
immunotherapeutic
approaches.
This
review
highlights
transformative
or-ganoid
models
advancing
preclinical
modeling,
evalua-tion,
paving
way
more
effective
treatments.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(19), P. 10503 - 10503
Published: Sept. 29, 2024
Around
13%
of
women
will
likely
develop
breast
cancer
during
their
lifetime.
Advances
in
metabolism
research
have
identified
a
range
metabolic
reprogramming
events,
such
as
altered
glucose
and
amino
acid
uptake,
increased
reliance
on
glycolysis,
interactions
with
the
tumor
microenvironment
(TME),
all
which
present
new
opportunities
for
targeted
therapies.
However,
studying
these
networks
is
challenging
traditional
2D
cell
cultures,
often
fail
to
replicate
three-dimensional
architecture
dynamic
real
tumors.
To
address
this,
organoid
models
emerged
powerful
tools.
Tumor
organoids
are
3D
derived
from
patient
tissue,
that
more
accurately
mimic
structural
functional
properties
actual
tissues
vivo,
offering
realistic
model
investigating
metabolism.
This
review
explores
unique
adaptations
discusses
how
can
provide
deeper
insights
into
processes.
We
evaluate
most
advanced
tools
culture
models,
including
optical
imaging
(OMI),
matrix-assisted
laser
desorption/ionization
mass
spectrometry
(MALDI-MSI),
recent
advances
conventional
techniques
applied
cultures.
Finally,
we
explore
progress
made
identifying
targeting
potential
therapeutic
targets
Medicine International,
Journal Year:
2024,
Volume and Issue:
5(2)
Published: Dec. 20, 2024
The
limitations
of
two‑dimensional
(2D)
models
in
cancer
research
have
hindered
progress
fully
understanding
the
complexities
drug
resistance
and
therapeutic
failures.
However,
three‑dimensional
(3D)
provide
a
more
accurate
representation
in
vivo
environments,
capturing
critical
cellular
interactions
dynamics
that
are
essential
evaluating
efficacy
toxicity
tyrosine
kinase
inhibitors
(TKIs).
These
advanced
enable
researchers
to
explore
mechanisms
with
greater
precision,
optimizing
treatment
strategies
improving
predictive
accuracy
clinical
outcomes.
By
leveraging
3D
models,
it
will
be
possible
deepen
current
TKIs
drive
forward
innovations
treatment.
present
review
discusses
2D
transformative
impact
on
oncology
research,
highlighting
their
roles
addressing
challenges
systems
advancing
TKI
studies.
