Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs) DOI Creative Commons
Balázs Széky, Veronika Juráková, Eliska Fouskova

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313514 - e0313514

Published: Dec. 4, 2024

Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. maintain neural metabolic support, synapse regulation, blood-brain barrier integrity immunological homeostasis through intricate interactions other cells, including neurons, microglia, pericytes lymphocytes. Due to their extensive intercellular crosstalks, astrocytes also implicated in pathogenesis CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson’s disease Alzheimer’s disease. Despite critical importance neurodegeneration neuroinflammation recognized, lack suitable vitro systems limits availability for modeling human brain pathologies. Here, we report time-efficient, reproducible generation from induced pluripotent stem cells (hiPSCs). Our hiPSC-derived expressed characteristic astrocyte markers, GFAP, S100b, ALDH1L1 AQP4. Furthermore, displayed spontaneous calcium transients responded inflammatory stimuli by secretion type A1 A2 astrocyte-related cytokines.

Language: Английский

Bisphenol A (BPA) and neurological disorders: An overview DOI Creative Commons
Sung‐Ae Hyun, Minhan Ka

The International Journal of Biochemistry & Cell Biology, Journal Year: 2024, Volume and Issue: 173, P. 106614 - 106614

Published: June 28, 2024

Language: Английский

Citations

9
Melatonin and resveratrol alleviate molecular and metabolic toxicity induced by Bisphenol A in endometrial organoids DOI Creative Commons
Mariam M. Abady, Islam M. Saadeldin, A. Han

et al.

Reproductive Toxicology, Journal Year: 2024, Volume and Issue: 128, P. 108628 - 108628

Published: June 5, 2024

Bisphenol A (BPA), a widespread environmental contaminant, poses concerns due to its disruptive effects on physiological functions of the uterine endometrium. In contrast, melatonin (MT) and Resveratrol (RSV) are under scrutiny for their potential protective roles against BPA-induced damage. For efficacy ethical in animal test, endometrial organoids, three-dimensional models mimicking endometrium, serve as crucial tools unraveling impact factors reproductive health. This study aimed comprehensively characterize morphological, molecular metabolic responses porcine organoids BPA assess MT RSV. Porcine uteri were prepared, digested with collagenase, mixed Matrigel, incubated at 38°C 5 % CO

Language: Английский

Citations

3
Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs) DOI Creative Commons
Balázs Széky, Veronika Juráková, Eliska Fouskova

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0313514 - e0313514

Published: Dec. 4, 2024

Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. maintain neural metabolic support, synapse regulation, blood-brain barrier integrity immunological homeostasis through intricate interactions other cells, including neurons, microglia, pericytes lymphocytes. Due to their extensive intercellular crosstalks, astrocytes also implicated in pathogenesis CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson’s disease Alzheimer’s disease. Despite critical importance neurodegeneration neuroinflammation recognized, lack suitable vitro systems limits availability for modeling human brain pathologies. Here, we report time-efficient, reproducible generation from induced pluripotent stem cells (hiPSCs). Our hiPSC-derived expressed characteristic astrocyte markers, GFAP, S100b, ALDH1L1 AQP4. Furthermore, displayed spontaneous calcium transients responded inflammatory stimuli by secretion type A1 A2 astrocyte-related cytokines.

Language: Английский

Citations

0