foxg1a is required for hair cell development and regeneration in the zebrafish lateral line DOI Creative Commons
J. Bell, Emily M. Turner, Cole Biesemeyer

et al.

Biology Open, Journal Year: 2024, Volume and Issue: 13(9)

Published: Sept. 15, 2024

ABSTRACT Mechanosensory hair cells located in the inner ear mediate sensations of hearing and balance. If damaged, mammalian are unable to regenerate, resulting permanent sensory deficits. Aquatic vertebrates like zebrafish (Danio rerio) have a specialized class mechanosensory found lateral line system, allowing them sense changes water current. Unlike cells, can robustly regenerate following damage. In mammals, transcription factor Foxg1 functions promote normal development ear. Foxg1a is expressed organs larvae, but its function during regeneration has not been investigated. Our study demonstrates that mutation foxg1a results slower posterior primordium migration delayed neuromast formation. developing regenerating neuromasts, we find loss reduced cell numbers, as well decreased proliferation cells. specifically regulates Islet1-labeled These data suggest may be valuable target for investigation clinical regeneration.

Language: Английский

Molecular insights into electroreceptor ribbon synapses from differential gene expression in sturgeon lateral line organs DOI Creative Commons
Alexander S. Campbell, Martin Minařík, David Buckley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract In fishes and aquatic-stage amphibians, mechanosensory neuromasts are arranged in characteristic lines the skin of head trunk, with afferent innervation from anterior or posterior lateral line nerves. electroreceptive non-teleost jawed fields electrosensory ampullary organs flank some all cranial neuromast lines, innervated by nerve. Like hair cells found inner ear, electroreceptor form specialised ribbon synapses nerve terminals. Ribbon distinct other glutamatergic synapses, including photoreceptors: otoferlin is Ca 2+ sensor for synaptic vesicle exocytosis vesicles loaded glutamate vGlut3. We previously showed that genes encoding vGlut3 expressed as well a chondrostean ray-finned fish, Mississippi paddlefish ( Polyodon spathula ), suggesting very similar to those cells. this study, we selected seven additional synapse-related candidate our published dataset putatively organ-enriched late-larval paddlefish, examined their expression developing related chondrostean, sterlet sturgeon Acipenser ruthenus ). presynaptic cell adhesion molecule Nrxn3, calcium-independent synaptotagmin Syt14, high-affinity re-uptake transporter EAAT1 (GLAST), calmodulin regulator protein PCP4 (PEP-19) DSCAML1 were both organs. contrast, Cbln18 , secreted trans-synaptic scaffolding protein, was only Tulp1 tubby-related 1 (required development function photoreceptor synapses), Our results support being suggest further commonalities, but also differences, synapses.

Language: Английский

Citations

0
The sensory shark: high-quality morphological, genomic and transcriptomic data for the small-spotted catsharkScyliorhinus caniculareveal the molecular bases of sensory organ evolution in jawed vertebrates DOI Open Access

Hélène Mayeur,

Jake Leyhr, John F. Mulley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Abstract Cartilaginous fishes (chimaeras and elasmobranchs -sharks, skates rays) hold a key phylogenetic position to explore the origin diversifications of jawed vertebrates. Here, we report integrate reference genomic, transcriptomic morphological data in small-spotted catshark Scyliorhinus canicula shed light on evolution sensory organs. We first characterise general aspects genome, confirming high conservation genome organisation across cartilaginous fishes, investigate population genomic signatures. Taking advantage dense sampling data, also identify gene signatures for all major organs, including chondrichthyan specializations, evaluate expression between paralogs within families involved functions. Finally, combine these with 3D synchrotron imaging situ analyses chondrichthyan-specific traits more evolutionary trends systems. This approach brings light, among others, novel markers ampullae Lorenzini electro-sensory cells, duplication hotspot crystallin genes conserved vertebrates, new metazoan clade Transient-receptor potential (TRP) family. These resources results, obtained an experimentally tractable model, open avenues multiomics study

Language: Английский

Citations

2
The sensory shark: high-quality morphological, genomic and transcriptomic data for the small-spotted catshark Scyliorhinus canicula reveal the molecular bases of sensory organ evolution in jawed vertebrates DOI Creative Commons

Hélène Mayeur,

Jake Leyhr, John F. Mulley

et al.

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract Cartilaginous fishes (chondrichthyans: chimaeras and elasmobranchs -sharks, skates rays) hold a key phylogenetic position to explore the origin diversifications of jawed vertebrates. Here, we report integrate reference genomic, transcriptomic morphological data in small-spotted catshark Scyliorhinus canicula shed light on evolution sensory organs. We first characterise general aspects genome, confirming high conservation genome organisation across cartilaginous fishes, investigate population genomic signatures. Taking advantage dense sampling data, also identify gene signatures for all major organs, including chondrichthyan specializations, evaluate expression between paralogs within families involved functions. Finally, combine these with 3D synchrotron imaging situ analyses chondrichthyan-specific traits more evolutionary trends systems. This approach brings light, among others, novel markers ampullae Lorenzini electro-sensory cells, duplication hotspot crystallin genes conserved vertebrates, new metazoan clade transient-receptor potential (TRP) family. These resources results, obtained an experimentally tractable model, open avenues multiomics study

Language: Английский

Citations

2
Two opposing roles for Bmp signalling in the development of electrosensory lateral line organs DOI Creative Commons
Alexander S. Campbell, Martin Minařík, Roman Franěk

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 12, 2024

Abstract The lateral line system enables all fishes and aquatic-stage amphibians to detect local water movement via mechanosensory hair cells in neuromasts, many species weak electric fields electroreceptors (modified cells) ampullary organs. Both neuromasts organs develop from placodes. However, the molecular mechanisms underpinning organ formation are understudied relative as ancestral lineages of zebrafish (teleosts) Xenopus (frogs) independently lost electroreception. We identified Bmp5 a promising candidate differential RNA-seq an electroreceptive ray-finned fish, Mississippi paddlefish ( Polyodon spathula ; Modrell et al., 2017, eLife 6: e24197). In experimentally tractable relative, sterlet sturgeon Acipenser ruthenus ), we found that four other Bmp pathway genes expressed developing line, signalling is active. Furthermore, CRISPR/Cas9-mediated mutagenesis targeting G0-injected embryos resulted fewer Conversely, when was inhibited by DMH1 treatment shortly before primordia, supernumerary developed. These data suggest promotes development, whereas another ligand(s) prevents their overproduction. Taken together, this demonstrates two opposing roles for during formation.

Language: Английский

Citations

1
foxg1ais required for hair cell development and regeneration in the zebrafish lateral line DOI Open Access
J. Bell, Cole Biesemeyer,

Emily M Turner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Abstract Mechanosensory hair cells located in the inner ear mediate sensations of hearing and balance. If damaged, mammalian are unable to regenerate, resulting permanent sensory deficits. Aquatic vertebrates like zebrafish (Danio rerio) have a specialized class mechanosensory found lateral line system, allowing them sense changes water current. Unlike cells, can robustly regenerate following damage. In models, transcription factor Foxg1 functions promote normal development ear. Foxg1a is expressed organs larvae, but its function during regeneration has not been investigated. We find that loss results reduced cell regeneration, as well decreased cellular proliferation system. These data suggest may be valuable target for investigation clinical regeneration. Summary statement Our work demonstrates role developing regenerating new through proliferation.

Language: Английский

Citations

0
foxg1a is required for hair cell development and regeneration in the zebrafish lateral line DOI Creative Commons
J. Bell, Emily M. Turner, Cole Biesemeyer

et al.

Biology Open, Journal Year: 2024, Volume and Issue: 13(9)

Published: Sept. 15, 2024

ABSTRACT Mechanosensory hair cells located in the inner ear mediate sensations of hearing and balance. If damaged, mammalian are unable to regenerate, resulting permanent sensory deficits. Aquatic vertebrates like zebrafish (Danio rerio) have a specialized class mechanosensory found lateral line system, allowing them sense changes water current. Unlike cells, can robustly regenerate following damage. In mammals, transcription factor Foxg1 functions promote normal development ear. Foxg1a is expressed organs larvae, but its function during regeneration has not been investigated. Our study demonstrates that mutation foxg1a results slower posterior primordium migration delayed neuromast formation. developing regenerating neuromasts, we find loss reduced cell numbers, as well decreased proliferation cells. specifically regulates Islet1-labeled These data suggest may be valuable target for investigation clinical regeneration.

Language: Английский

Citations

0