Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158616 - 158616
Published: Dec. 1, 2024
npj Biological Physics and Mechanics., Journal Year: 2025, Volume and Issue: 2(1)
Published: Feb. 4, 2025
Abstract The cancer metastatic cascade includes a series of mechanical barrier-crossing events, involving the physical movement cells from their primary location to distant organ. This review describes changes that influence tumour proliferation, progression, and metastasis. We identify potential signatures at every step discuss some latest mechanobiology-based therapeutic interventions highlight importance interdisciplinary approaches in diagnosis treatment.
Language: Английский
Citations
4
Biomedical Engineering Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100143 - 100143
Published: Jan. 1, 2025
Language: Английский
Citations
3
Bulletin of Mathematical Biology, Journal Year: 2025, Volume and Issue: 87(3)
Published: Feb. 12, 2025
Abstract Extracellular matrix (ECM) is a key component of the cellular microenvironment and critical in multiple disease developmental processes. Representing ECM cell-ECM interactions challenging multiscale problem as they span molecular-level details to tissue-level dynamics. While several computational frameworks exist for modeling, often focus on very detailed modeling individual fibers or represent only single aspect ECM. Using PhysiCell agent-based platform, we developed framework intermediate detail with ability capture bidirectional interactions. We small region ECM, an element, three variables describing its local microstructure: anisotropy, density, overall fiber orientation. To spatially model use array elements. Cells remodel microstructure turn, impacts motility. demonstrate utility this reusability core interaction through examples invasion, wound healing, basement membrane degradation, leader-follower collective migration. Despite relative simplicity framework, it able broad range interest community. Furthermore, representing are accessible simple programming interfaces. This allows them impact cell behaviors, such proliferation death, without requiring custom code each interaction, particularly PhysiCell’s grammar, enabling rapid diverse cell-matrix biology. make available free open source software package at https://github.com/PhysiCell-Models/collective-invasion .
Language: Английский
Citations
1
Current Opinion in Structural Biology, Journal Year: 2024, Volume and Issue: 89, P. 102919 - 102919
Published: Sept. 21, 2024
Language: Английский
Citations
8
Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12
Published: Jan. 6, 2025
Many mammalian cells, including endothelial cells and fibroblasts, align elongate along the orientation of extracellular matrix (ECM) fibers in a gel when cultured vitro. During cell elongation, clusters focal adhesions (FAs) form near poles elongating cells. FAs are mechanosensitive that grow under mechanical tension exerted by cells' pulling on ECM shrink is released. In this study, we use mathematical modeling to study hypothesis reciprocity between sufficient for directing shape changes orientation. We show preferentially stabilized fibers, where can generate higher than directions perpendicular fibers. present hybrid computational model coupling three approaches: first, cellular Potts (CPM) describes an individual contractile cell; second, molecular dynamics (MD) represent as network cross-linked, deformable fibers; third, set ordinary differential equations (ODEs) cell's FAs, terms balance assembly mechanoresponsive disassembly. The resulting shows suffices stiffness-dependent spreading, local remodeling, ECM-alignment-dependent elongation. These combined effects explain how morphology influenced structure mechanics.
Language: Английский
Citations
0
Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13
Published: April 9, 2025
Metastasis, the leading cause of death in cancer patients, arises when cells disseminate from a primary solid tumour to distant organs. Growth and invasion often involve collective cell migration, which is profoundly influenced by cell-cell interactions extracellular matrix (ECM). The ECM's biochemical composition mechanical properties, such as stiffness, regulate behaviour migration dynamics. Mathematical modelling serves pivotal tool for studying predicting these complex dynamics, with hybrid discrete-continuous models offering powerful approach combining agent-based representations continuum descriptions surrounding microenvironment. In this study, we investigate impact ECM modulated via ribose-induced collagen cross-linking, on spheroid growth invasion. We employed model implemented PhysiCell simulate successfully replicating three-dimensional vitro experiments. incorporates detailed cell-ECM interactions, remodelling, proliferation. Our simulations align experimental observations two breast lines, non-invasive MCF7 invasive HCC 1954, under varying stiffness conditions. results demonstrate that increased due cross-linking inhibits cells, whereas remain largely unaffected. Furthermore, our show higher degradation not only enables but also facilitates formation multicellular protrusions. Conversely, increasing maximum speed can reach enhances while promoting single-cell This understanding interplay between proliferation, paving way future studies incorporating additional characteristics microenvironmental
Language: Английский
Citations
0
Gigabyte, Journal Year: 2024, Volume and Issue: 2024
Published: Oct. 16, 2024
The extracellular matrix, composed of macromolecules like collagen fibres, provides structural support to cells and acts as a barrier that metastatic degrade spread beyond the primary tumour. While agent-based frameworks, such PhysiCell, can simulate spatial dynamics tumour evolution, they only implement circles (2D) or spheres (3D). To model matrix network we require new type agent represented by line segments cylinders Here, present PhysiMeSS, an addon introducing describe fibres their physical interactions with other fibres. PhysiMeSS implementation is available at https://github.com/PhysiMeSS/PhysiMeSS in official PhysiCell repository. We provide examples describing possibilities this framework. This tool may help tackle important biological questions, diseases linked dysregulation processes leading cancer metastasis.
Language: Английский
Citations
3
Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 11, 2025
Coordinated cell rotation along a curved matrix interface can sculpt epithelial tissues into spherical morphologies. Subsequently, radially-oriented invasion of multicellular strands or branches occur by local remodeling the confining matrix. These symmetry-breaking transitions emerge from dynamic reciprocity between cells and matrix, but remain poorly understood. Here, we show that spheroids collectively transition circumferential orbiting to radial via bi-directional interactions with surrounding curvature. Initially, exhibit an ellipsoidal shape become rounded as occurs. However, gradually reorient coordinated towards outward strand due accumulation contractile tractions at discrete sites. Remarkably, initial ellipsoid morphology predicts subsequent 2-4 roughly aligned major axis. We then perturb collective migration using osmotic pressure, showing be arrested reversed. also investigate in "mosaic" spheroids, small fraction "leader" weakened cell-cell adhesions impede still invade Finally, establish minimal self-propelled particle model elucidate how is mediated crosstalk cell-matrix adhesion boundary. Altogether, this work elucidates tissue morphogenesis governed interplay behaviors curvature cell-matrix, relevance for embryonic development tumor progression.
Language: Английский
Citations
0
Journal of Differential Equations, Journal Year: 2025, Volume and Issue: 428, P. 721 - 746
Published: Feb. 14, 2025
Language: Английский
Citations
0