Tenascin-C from the tissue microenvironment promotes muscle stem cell self-renewal through Annexin A2 DOI Creative Commons

Mafalda Loreti,

Alessandra Cecchini,

Collin D. Kaufman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Skeletal muscle tissue self-repair occurs through the finely timed activation of resident stem cells (MuSC). Following perturbation, MuSC exit quiescence, undergo myogenic commitment, and differentiate to regenerate injured muscle. This process is coordinated by signals present in microenvironment, however precise mechanisms which microenvironment regulates are still poorly understood. Here, we identified Tenascin-C (TnC), an extracellular matrix (ECM) glycoprotein, as a key player promoting self-renewal function. We show that fibro-adipogenic progenitors (FAPs) primary cellular source TnC during repair, sense signaling cell surface receptor Annexin A2. provide vivo evidence required for efficient mice lacking exhibit regeneration phenotype premature aging. propose decline physiological aging contributes inefficient aged Taken together, our results highlight pivotal role repair healthy skeletal

Language: Английский

Analyzing Muscle Stem Cell Function Ex Vivo DOI
Julia von Maltzahn

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
The Role of Primary Cilia in Myoblast Proliferation and Cell Cycle Regulation during Myogenesis DOI Creative Commons
Zhichao Wu, Nuo Chen, Daisuke Takao

et al.

Cell Structure and Function, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The process of mammalian myogenesis is fundamental to understanding muscle development and holds broad relevance across multiple fields, from developmental biology regenerative medicine. This review highlights two key aspects: myoblast proliferation the role cilia in this process. Myoblasts, as precursor cells, must undergo tightly regulated cycles differentiation ensure proper growth function. Recent research has uncovered an essential for primary cilia, hair-like sensory organelles on cell surface, modulating signaling pathways crucial myogenesis. Cilium-mediated appears regulate various stages myogenesis, including control differentiation. Furthermore, formation disassembly during presumably enabling detailed, context-dependent regulation their functions. In particular, through cycle by important topic that requires further investigation. By examining interactions between myoblasts, aims provide new insights into molecular cellular mechanisms driving development, with potential applications muscle-related diseases advancing therapeutic strategies. Additionally, advancements imaging image analysis technologies have become indispensable studying these processes at level. also addresses technological current challenges.Key words: myoblast, proliferation, imaging.

Language: Английский

Citations

0
MESENCHYMAL STEM CELLS AND FIBROBLASTS IN AGE-RELATED REMODELING OF THE MUSCULOSKELETAL SYSTEM DOI
Н. Г. Плехова, Polina A. Krivolutskaya, Shumatov V.B.

et al.

Bulletin Biomedicine and sociology, Journal Year: 2024, Volume and Issue: unknown, P. 33 - 43

Published: Sept. 30, 2024

Mesenchymal stem/stromal cells (MSCs) and fibroblasts are present in normal tissues to maintain tissue homeostasis share a number of common features such as spindle-shaped morphology, localization connective tissue, multipotency. During inflammation, MSCs non-specifically respond injury via two mechanisms action: immunomodulation regeneration. Upon injury, activated, proliferate, differentiate. With age and, particular, degenerative diseases the musculoskeletal system (diseases muscles, joints bones), regenerative capacity is lost or redirected production other non-functional cell types adipocytes fibroblasts, which provide much structural framework almost all types. By performing an immunosuppressive role, contribute resolution prerequisite for successful repair. The aim review understanding opposite properties FB from standpoint age-related changes order develop approaches their complementary assistance key aging MSC fibroblast presented, it indicated that additional studies needed on cellular together generate dysfunctions individual niches terminal differentiated during aging. currently existing technologies therapy involving presented.

Language: Английский

Citations

0
Tenascin-C from the tissue microenvironment promotes muscle stem cell self-renewal through Annexin A2 DOI Creative Commons

Mafalda Loreti,

Alessandra Cecchini,

Collin D. Kaufman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Skeletal muscle tissue self-repair occurs through the finely timed activation of resident stem cells (MuSC). Following perturbation, MuSC exit quiescence, undergo myogenic commitment, and differentiate to regenerate injured muscle. This process is coordinated by signals present in microenvironment, however precise mechanisms which microenvironment regulates are still poorly understood. Here, we identified Tenascin-C (TnC), an extracellular matrix (ECM) glycoprotein, as a key player promoting self-renewal function. We show that fibro-adipogenic progenitors (FAPs) primary cellular source TnC during repair, sense signaling cell surface receptor Annexin A2. provide vivo evidence required for efficient mice lacking exhibit regeneration phenotype premature aging. propose decline physiological aging contributes inefficient aged Taken together, our results highlight pivotal role repair healthy skeletal

Language: Английский

Citations

0