From bone marrow mesenchymal stem cells to diseases: the crucial role of m6A methylation in orthopedics DOI Creative Commons
Jing Li, Chu Zhang, Wen Yin

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 6, 2025

Elucidating the molecular mechanisms underlying orthopedic diseases is crucial for guiding therapeutic strategies and developing innovative interventions. N6-methyladenosine (m6A)-an epitranscriptomic modification-has emerged as a key regulator of cellular fate tissue homeostasis. Specifically, m6A plays pivotal role in several RNA biological processes such precursor splicing, 3'-end processing, nuclear export, translation, stability. Recent advancements indicate that methylation regulates stem cell proliferation osteogenic differentiation by modulating various signaling pathways. Extensive research has shown abnormalities contribute significantly to onset progression osteoporosis (OP), osteoarthritis (OA), rheumatoid arthritis (RA), bone tumors. This review aims summarize proteases involved their functions. The detailed which marrow mesenchymal cells (BMSCs) through direct indirect ways are also discussed, with focus on specific Finally, this analyzes roles modification development multiple diseases, offering comprehensive understanding pathophysiology these conditions proposing new directions targets treatment strategies.

Language: Английский

Electroacupuncture regulates FTO/Nrf2/NLRP3 axis-mediated pyroptosis in cerebral ischemia–reperfusion injury DOI
Chenglong Li, Haisheng Ji, Wei Mao

et al.

Neuroreport, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Electroacupuncture (EA) demonstrates neuroprotective in cerebral ischemia–reperfusion (I/R) injury. N6-methyladenosine (m6A) is found to contribute the pathogenesis of neurological conditions recently. The objective this study investigate effects EA on m6A and related mechanism I/R After middle artery occlusion/reperfusion (MCAO/R) operation was used establish rat models with injury, applied Baihui (GV20) Dazhui (GV14) once daily for 7 consecutive days. Subsequently, modified Neurological Severity Score, 2,3,5-triphenyltetrazolium chloride staining, hematoxylin eosin staining were performed assess damage. To potential target, total RNA level relevant regulators (METTL3, METTL14, WTAP, FTO, ALKBH5) examined. In next step, Nrf2, NLRP3, IL-18, IL-1β, TUNEL-positive rates detected, while shRNA-FTO administered suppress FTO expression. improved neurobehavioral disorders, infarct volume, pathological damage induced by Mechanically, reduced selectively regulating but not METTL3, ALKBH5. Furthermore, could enhance Nrf2 rates, which reversed injection. Our findings indicate that may alleviate FTO/Nrf2/NLRP3 axis-mediated pyroptosis providing a more unified understanding EA. Specifically, intervention appears promote expression leading reduction level, activates subsequently suppresses NLRP3-mediated pyroptosis.

Language: Английский

Citations

0
A comprehensive review of m6 A methylation in coronary heart disease DOI
Mei-Ning Diao, Yingli Lv, Xin Hui

et al.

Journal of Molecular Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский

Citations

0
From bone marrow mesenchymal stem cells to diseases: the crucial role of m6A methylation in orthopedics DOI Creative Commons
Jing Li, Chu Zhang, Wen Yin

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 6, 2025

Elucidating the molecular mechanisms underlying orthopedic diseases is crucial for guiding therapeutic strategies and developing innovative interventions. N6-methyladenosine (m6A)-an epitranscriptomic modification-has emerged as a key regulator of cellular fate tissue homeostasis. Specifically, m6A plays pivotal role in several RNA biological processes such precursor splicing, 3'-end processing, nuclear export, translation, stability. Recent advancements indicate that methylation regulates stem cell proliferation osteogenic differentiation by modulating various signaling pathways. Extensive research has shown abnormalities contribute significantly to onset progression osteoporosis (OP), osteoarthritis (OA), rheumatoid arthritis (RA), bone tumors. This review aims summarize proteases involved their functions. The detailed which marrow mesenchymal cells (BMSCs) through direct indirect ways are also discussed, with focus on specific Finally, this analyzes roles modification development multiple diseases, offering comprehensive understanding pathophysiology these conditions proposing new directions targets treatment strategies.

Language: Английский

Citations

0