Coactosin-like F-actin binding protein (Cotl1) plays a key role in adipocyte differentiation and obesity DOI Creative Commons
Eunkuk Park,

Hyoju Jeon,

Kang-Il Oh

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 17, 2025

Actin dynamics, mediated by various actin-binding proteins, plays an important role in adipocyte differentiation. We investigated the of coactosin-like F-actin binding protein (Cotl1) differentiation vitro and vivo. Cotl1 expression level was increased during mouse 3T3-L1 cells primary cultured adipose-derived stem (ADSCs) weight gain adipose tissues. However, deficient ADSCs inhibited differentiation, Cotl1-/- mice displayed resistance to high-fat diet (HFD)-induced gain, hepatic steatosis enlargement compared HFD-fed wild type (WT) mice. Ingenuity Pathway Analysis RNA-sequencing tissues HFD-WT HFD-Cotl1-/- predicted complicated relationships between Cotl1, adipocytes, obesity organization actin cytoskeleton. Particularly, peroxisome proliferator-activated receptor gamma (Pparg) emerged as a central player, with influencing Pparg expression, consequently regulating These findings suggest pivotal regulator terminal modulating adipogenic genes.

Language: Английский

Coactosin-like F-actin binding protein (Cotl1) plays a key role in adipocyte differentiation and obesity DOI Creative Commons
Eunkuk Park,

Hyoju Jeon,

Kang-Il Oh

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: April 17, 2025

Actin dynamics, mediated by various actin-binding proteins, plays an important role in adipocyte differentiation. We investigated the of coactosin-like F-actin binding protein (Cotl1) differentiation vitro and vivo. Cotl1 expression level was increased during mouse 3T3-L1 cells primary cultured adipose-derived stem (ADSCs) weight gain adipose tissues. However, deficient ADSCs inhibited differentiation, Cotl1-/- mice displayed resistance to high-fat diet (HFD)-induced gain, hepatic steatosis enlargement compared HFD-fed wild type (WT) mice. Ingenuity Pathway Analysis RNA-sequencing tissues HFD-WT HFD-Cotl1-/- predicted complicated relationships between Cotl1, adipocytes, obesity organization actin cytoskeleton. Particularly, peroxisome proliferator-activated receptor gamma (Pparg) emerged as a central player, with influencing Pparg expression, consequently regulating These findings suggest pivotal regulator terminal modulating adipogenic genes.

Language: Английский

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