Lymphomonocytic Extracellular Vesicles Influence Fibroblast Proliferation and Collagen Production in Systemic Sclerosis DOI Open Access
Giuseppe Argentino, Bianca Olivieri,

Matteo Morandi

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2699 - 2699

Published: March 17, 2025

Systemic sclerosis (SSc) is a chronic autoimmune disorder characterized by fibrosis, immune dysregulation, and vascular abnormalities. Extracellular vesicles (EVs), secreted cells, have been implicated in modulating fibroblast activity are actively involved SSc pathogenesis. This study aims to determine whether lymphomonocytic-derived EVs influence proliferation collagen synthesis SSc. Fibroblasts from healthy donors (HDFs) patients (SScHDFs) were exposed derived Jurkat U937 cell lines stimulated under pro-inflammatory conditions using tumor necrosis factor-α (TNFα) or phorbol 12-myristate 13-acetate + ionomycin (PMA IONO). Proliferation was assessed CCK-8 assays, while production quantified via ELISA. Our findings demonstrate that PMA IONO-stimulated cells significantly reduced dose-dependent manner. Notably, SScHDFs exhibited lower baseline diminished overall response EV treatment. Collagen markedly both types following exposure EVs, whereas TNFα-stimulated affected only HDFs. These suggest activated modulate function SSc, potentially contributing disease Further research warranted elucidate the molecular mechanisms underlying these effects explore therapeutic potential of targeting EV-mediated signaling

Language: Английский

Lymphomonocytic Extracellular Vesicles Influence Fibroblast Proliferation and Collagen Production in Systemic Sclerosis DOI Open Access
Giuseppe Argentino, Bianca Olivieri,

Matteo Morandi

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2699 - 2699

Published: March 17, 2025

Systemic sclerosis (SSc) is a chronic autoimmune disorder characterized by fibrosis, immune dysregulation, and vascular abnormalities. Extracellular vesicles (EVs), secreted cells, have been implicated in modulating fibroblast activity are actively involved SSc pathogenesis. This study aims to determine whether lymphomonocytic-derived EVs influence proliferation collagen synthesis SSc. Fibroblasts from healthy donors (HDFs) patients (SScHDFs) were exposed derived Jurkat U937 cell lines stimulated under pro-inflammatory conditions using tumor necrosis factor-α (TNFα) or phorbol 12-myristate 13-acetate + ionomycin (PMA IONO). Proliferation was assessed CCK-8 assays, while production quantified via ELISA. Our findings demonstrate that PMA IONO-stimulated cells significantly reduced dose-dependent manner. Notably, SScHDFs exhibited lower baseline diminished overall response EV treatment. Collagen markedly both types following exposure EVs, whereas TNFα-stimulated affected only HDFs. These suggest activated modulate function SSc, potentially contributing disease Further research warranted elucidate the molecular mechanisms underlying these effects explore therapeutic potential of targeting EV-mediated signaling

Language: Английский

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