From Flies to Humans: Conserved Roles of CEBPZ, NOC2L, and NOC3L in rRNA Processing and Tumorigenesis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Abstract NOC1, NOC2, and NOC3 are conserved nucleolar proteins essential for regulating ribosomal RNA (rRNA) maturation, a process critical cellular homeostasis. in Drosophila yeast, enhances activity to sustain rRNA processing, whereas its depletion leads impaired polysome formation, reduced protein synthesis, apoptosis. These genes have vertebrate homologs called CEBPZ, NOC2L, NOC3l. In this study, we demonstrated that the RNA-regulatory functions of CEBPZ vertebrates, showed accumulation 45S-pre rRNA, with consequent reduction synthesis. Gene Ontology bioinformatic analyses NOC3L tumors highlight significant correlation between their processes regulate 60S maturation. Comparative analysis expression tumor databases revealed exhibit contrasting patterns across types. This dual role suggests overexpression promotes growth, may exert tumor-suppressive effects, uncovering unexpected regulatory exerted by these cancer.

Language: Английский

Nucleolar origins: challenging perspectives on evolution and function

Open Biology, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

The nucleolus, once considered a mere ‘ribosome factory’, is now recognized as dynamic hub influencing nearly every aspect of cellular life, from genome organization to stress response and ageing. Despite being hallmark eukaryotic cells, recent discoveries reveal that even prokaryotes exhibit nucleolus-like structures, hinting at ancient origins for nucleolar functions. This review explores the evolutionary journey tracing its roots back early life examining structural functional diversity across domains. We highlight key proteins play vital roles not only in ribosome production but also regulating cell cycle, DNA repair stress, linking activity directly health disease. Dysfunctions processes are implicated cancer, ribosomopathies neurodegenerative disorders, positioning nucleolus critical target innovative therapeutic strategies. As advanced imaging molecular techniques unlock deeper insights into both canonical mysterious non-canonical roles, stands model how microenvironments can evolve meet complex biological demands. By addressing open questions surrounding evolution diverse functions, ideas presented here aim contribute ongoing discussion, challenging traditional paradigms suggesting new avenues uncovering fundamental principles drive life.

Language: Английский