Investigation of c-Fos/c-Jun Signaling Pathways in Periostracum Cicadae’s Inhibition of EMT in Gastric Tissue DOI Creative Commons
Hua Liang,

Xiaofei Jin,

Tao He

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 537 - 537

Published: April 7, 2025

Background/Objectives: Periostracum Cicadae (PC) is commonly used to treat chronic atrophic gastritis (CAG), but its underlying mechanisms are unclear. We investigated the therapeutic effects, active ingredients and molecular of PC on CAG. Methods: analyzed components in serum extract by UHPLC-Q-Orbitrap-MS/MS. Then, we rat cell models assess impact CAG employed network pharmacology bioinformatics predict key targets ingredients. Finally, confirmed hub through experiments docking. Results: A total 22 were identified extract-containing using UHPLC-Q-Orbitrap MS/MS. Network combined with docking revealed that protective effect was primarily mediated three compounds: (Z)-akuammidine, chicoric acid, columbianadin. And c-Fos/c-Jun signaling pathways crucial therapy. inhibited vitality, migration, invasion, multiplication MC cells (model for CAG), induced apoptosis, caused G0/G1 phase cycle arrest. The expression level tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) gastrin 17 (G17) rats increased, while pepsinogen I (PG I) II II) decreased. After 12 weeks administration, these conditions significantly improved. not only reduced levels antigen KI-67 (Ki67) protein p53 (P53) also enhanced SRY-box Transcription Factor (SOX2). Simultaneously, down-regulated N-cadherin Vimentin up-regulating E-cadherin. Conclusions: epithelial–mesenchymal transition (EMT) via pathway, thereby providing benefits Our study elucidates material basis treating CAG, experimental evidence support clinical application.

Language: Английский

Probiotics and prebiotics: new treatment strategies for oral potentially malignant disorders and gastrointestinal precancerous lesions DOI Creative Commons

Zhuwei Huang,

Julia Zhu,

Xiaobo Bu

et al.

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 8, 2025

Oral potentially malignant disorders (OPMDs) and gastrointestinal precancerous lesions (GPLs) are major public health concerns because of their potential to progress cancer. Probiotics, prebiotics, engineered probiotics can positively influence the prevention management OPMDs GPLs. This review aims comprehensively application status probiotics, prebiotics in GPLs, explore mechanisms action, anticipate future clinical use.

Language: Английский

Citations

0
Investigation of c-Fos/c-Jun Signaling Pathways in Periostracum Cicadae’s Inhibition of EMT in Gastric Tissue DOI Creative Commons
Hua Liang,

Xiaofei Jin,

Tao He

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 537 - 537

Published: April 7, 2025

Background/Objectives: Periostracum Cicadae (PC) is commonly used to treat chronic atrophic gastritis (CAG), but its underlying mechanisms are unclear. We investigated the therapeutic effects, active ingredients and molecular of PC on CAG. Methods: analyzed components in serum extract by UHPLC-Q-Orbitrap-MS/MS. Then, we rat cell models assess impact CAG employed network pharmacology bioinformatics predict key targets ingredients. Finally, confirmed hub through experiments docking. Results: A total 22 were identified extract-containing using UHPLC-Q-Orbitrap MS/MS. Network combined with docking revealed that protective effect was primarily mediated three compounds: (Z)-akuammidine, chicoric acid, columbianadin. And c-Fos/c-Jun signaling pathways crucial therapy. inhibited vitality, migration, invasion, multiplication MC cells (model for CAG), induced apoptosis, caused G0/G1 phase cycle arrest. The expression level tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) gastrin 17 (G17) rats increased, while pepsinogen I (PG I) II II) decreased. After 12 weeks administration, these conditions significantly improved. not only reduced levels antigen KI-67 (Ki67) protein p53 (P53) also enhanced SRY-box Transcription Factor (SOX2). Simultaneously, down-regulated N-cadherin Vimentin up-regulating E-cadherin. Conclusions: epithelial–mesenchymal transition (EMT) via pathway, thereby providing benefits Our study elucidates material basis treating CAG, experimental evidence support clinical application.

Language: Английский

Citations

0