Optimizing Factors in Murine Whole-Organ Cochlea Culture DOI Open Access
Andrea Tröger, Werner Bader, Timo Gottfried

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3908 - 3908

Published: April 21, 2025

In 2008, Hahn et al. presented a method for cultivating 3D organ culture of the cochlea. Although this is well established, it currently only applied to early postnatal animals. Given known differences in regeneration and repair abilities between adult mammalian cochleae, our goal was further develop optimize extend beyond animals include cochleae. After rapidly dissecting cochlea, opened placed neurotrophin-containing medium. The then maintained at 32 °C rotating bioreactor 24 h. combination mild hypothermia (32 °C), quick cochlea dissection, addition 10 ng/mL Brain-derived neurotrophic factor (Bdnf) 5 Neurotrophin 3 (Ntf3) medium ensures complete cell survival all cochlear types 10-day-old mice. modifications established incorporation neurotrophins (Bdnf Ntf3) into cultivation under hypothermic conditions °C). By introducing °C, can also be with This vitro model preserves similar those found vivo.

Language: Английский

The Blood-Labyrinth Barrier: Non-Invasive Delivery Strategies for Inner Ear Drug Delivery DOI Creative Commons
Zhangyi Yi, Xiaoying Wang,

Ge Yin

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 482 - 482

Published: April 7, 2025

The inner ear is a relatively isolated organ, protected by the blood-labyrinth barrier (BLB). This creates unique lymphatic fluid environment within ear, maintaining stable physiological state essential for mechano-electrical transduction process in hair cells while simultaneously restricting most drugs from entering fluid. Under pathological conditions, dysfunction of stria vascularis and disruption structure can lead to temporary or permanent hearing impairment. review describes function BLB, along with recent advancements modeling protective studies related BLB. emphasizes some newly developed non-invasive drug delivery strategies, including ultrasound therapy assisted microbubbles, ear-targeting peptides, sound therapy, route administration cerebrospinal conduit. We argue that intrinsic properties BLB be strategically utilized effective delivery.

Language: Английский

Citations

0
Optimizing Factors in Murine Whole-Organ Cochlea Culture DOI Open Access
Andrea Tröger, Werner Bader, Timo Gottfried

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3908 - 3908

Published: April 21, 2025

In 2008, Hahn et al. presented a method for cultivating 3D organ culture of the cochlea. Although this is well established, it currently only applied to early postnatal animals. Given known differences in regeneration and repair abilities between adult mammalian cochleae, our goal was further develop optimize extend beyond animals include cochleae. After rapidly dissecting cochlea, opened placed neurotrophin-containing medium. The then maintained at 32 °C rotating bioreactor 24 h. combination mild hypothermia (32 °C), quick cochlea dissection, addition 10 ng/mL Brain-derived neurotrophic factor (Bdnf) 5 Neurotrophin 3 (Ntf3) medium ensures complete cell survival all cochlear types 10-day-old mice. modifications established incorporation neurotrophins (Bdnf Ntf3) into cultivation under hypothermic conditions °C). By introducing °C, can also be with This vitro model preserves similar those found vivo.

Language: Английский

Citations

0