Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level DOI Creative Commons
Rahuljeet Chadha, Jason A. Guerrero, Lu Wei

et al.

ACS Central Science, Journal Year: 2024, Volume and Issue: unknown

Published: March 21, 2024

This outlook explores how two different molecular imaging approaches might be combined to gain insight into dynamic, subcellular metabolic processes. Specifically, we discuss matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI) and stimulated Raman scattering (SRS) microscopy, which have significantly pushed the boundaries of metabolomic analyses in their own right, could create comprehensive images. We first briefly summarize recent advances for each technique. then explore one overcome inherent limitations individual method, by envisioning orthogonal interchangeable workflows. Additionally, delve potential benefits adopting a complementary approach that combines both MSI SRS spectro-microscopy informing on specific chemical structures through functional-group-specific targets. Ultimately, integrating strengths modalities, researchers can achieve more understanding biological systems, enabling precise investigations. synergistic holds substantial promise expand our toolkit studying metabolites complex environments.

Language: Английский

Methods and applications for single-cell and spatial multi-omics DOI Open Access
Katy Vandereyken, Alejandro Sifrim, Bernard Thienpont

et al.

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 24(8), P. 494 - 515

Published: March 2, 2023

Language: Английский

Citations

606
Single-cell and spatial multi-omics in the plant sciences: Technical advances, applications, and perspectives DOI Creative Commons

Xiaole Yu,

Zhixin Liu, Xuwu Sun

et al.

Plant Communications, Journal Year: 2022, Volume and Issue: 4(3), P. 100508 - 100508

Published: Dec. 20, 2022

Plants contain a large number of cell types and exhibit complex regulatory mechanisms. Studies at the single-cell level have gradually become more common in plant science. Single-cell transcriptomics, spatial metabolomics techniques been combined to analyze development. These used study transcriptomes metabolomes tissues level, enabling systematic investigation gene expression metabolism specific during defined developmental stages. In this review, we present an overview significant breakthroughs multi-omics plants, discuss how these approaches may soon play essential roles research.

Language: Английский

Citations

58
Quantitative mass spectrometry imaging: therapeutics & biomolecules DOI Creative Commons
Joseph Holbrook,

Gabrielle E. Kemper,

Amanda B. Hummon

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(16), P. 2137 - 2151

Published: Jan. 1, 2024

In this feature article, we discuss quantitative mass spectrometry imaging of therapeutics and biomolecules conducted by recent studies for matrix-assisted laser desorption/ionization (MALDI) desorption electrospray ionization (DESI) techniques.

Language: Английский

Citations

15
A review of current trends of vitamin identification and quantification by chromatography from food samples DOI
Ionuț Avrămia,

Mircea-Adrian Oroian,

Radu-Cristian Oiţă

et al.

Journal of Food Composition and Analysis, Journal Year: 2024, Volume and Issue: 131, P. 106244 - 106244

Published: April 10, 2024

Language: Английский

Citations

9
Proteomic Insight Into Alzheimer's Disease Pathogenesis Pathways DOI Creative Commons

Taekyung Ryu,

Kyungdo Kim, Nicholas Asiimwe

et al.

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

ABSTRACT Alzheimer's disease (AD) is a leading cause of dementia, but the pathogenesis mechanism still elusive. Advances in proteomics have uncovered key molecular mechanisms underlying AD, revealing complex network dysregulated pathways, including amyloid metabolism, tau pathology, apolipoprotein E (APOE), protein degradation, neuroinflammation, RNA splicing, metabolic dysregulation, and cognitive resilience. This review examines recent proteomic findings from AD brain tissues biological fluids, highlighting potential biomarkers therapeutic targets. By examining landscape them, we aim to deepen our understanding support developing precision medicine strategies for more effective interventions.

Language: Английский

Citations

1
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) for in situ analysis of endogenous small molecules in biological samples DOI
Huihui Liu, Yu Pan, Caiqiao Xiong

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 157, P. 116809 - 116809

Published: Nov. 1, 2022

Language: Английский

Citations

38
A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids DOI Creative Commons
Jenny Leopold, Patricia Prabutzki,

Kathrin M. Engel

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(3), P. 546 - 546

Published: March 16, 2023

Matrix-assisted laser desorption and ionization (MALDI) is a widely used soft-ionization technique of modern mass spectrometry (MS). MALDI enables the analysis nearly all chemical compounds—including polar apolar (phospho)lipids—with minimum extent fragmentation. has some particular advantages (such as possibility to acquire spatially-resolved spectra) competitive with simultaneously developed ESI (electrospray ionization) MS. Although there are still methodological aspects that need be elucidated in more detail, it obvious careful selection an appropriate matrix plays most important role (lipid) analysis. Some lipid classes can detected exclusively if optimum used, determines sensitivity by which within mixture. Since is, thus, crucial for results, we provide here update on progress field since our original review this journal 2018. Thus, only development during last five years considered, lipids sorted according increasing complexity, starting free fatty acids ending cardiolipins phosphoinositides.

Language: Английский

Citations

22
Microscopy-Directed Imaging Mass Spectrometry for Rapid High Spatial Resolution Molecular Imaging of Glomeruli DOI
Allison B. Esselman, Nathan Heath Patterson, Lukasz G. Migas

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2023, Volume and Issue: 34(7), P. 1305 - 1314

Published: June 15, 2023

The glomerulus is a multicellular functional tissue unit (FTU) of the nephron that responsible for blood filtration. Each contains multiple substructures and cell types are crucial their function. To understand normal aging disease in kidneys, methods high spatial resolution molecular imaging within these FTUs across whole slide images required. Here we demonstrate workflow using microscopy-driven selected sampling to enable 5 μm pixel size matrix-assisted laser desorption/ionization mass spectrometry (MALDI IMS) all glomeruli human kidney tissues. Such entails large numbers pixels, increasing data acquisition times. Automating FTU-specific enables high-resolution analysis critical structures, while concurrently maintaining throughput. Glomeruli were automatically segmented coregistered autofluorescence microscopy data, segmentations translated into MALDI IMS measurement regions. This allowed high-throughput 268 from single section. Unsupervised machine learning used discover profiles glomerular subregions differentiate between healthy diseased glomeruli. Average spectra each analyzed Uniform Manifold Approximation Projection (UMAP) k-means clustering, yielding 7 distinct groups differentiated Pixel-wise clustering was applied glomeruli, showing unique localized glomerulus. Automated microscopy-driven, FTU-targeted maintains rapid assessment at cellular identification features associated with disease.

Language: Английский

Citations

22
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling DOI
Patric Bourceau, Benedikt Geier,

Vincent Suerdieck

et al.

Nature Protocols, Journal Year: 2023, Volume and Issue: 18(10), P. 3050 - 3079

Published: Sept. 6, 2023

Language: Английский

Citations

22
Advances in Mass Spectrometry-Based Single Cell Analysis DOI Creative Commons

Siheun Lee,

Hung M. Vu, Jung‐Hyun Lee

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(3), P. 395 - 395

Published: March 2, 2023

Technological developments and improvements in single-cell isolation analytical platforms allow for advanced molecular profiling at the level, which reveals cell-to-cell variation within admixture cells complex biological or clinical systems. This helps to understand cellular heterogeneity of normal diseased tissues organs. However, most studies focused on analysis nucleic acids (e.g., DNA RNA) mass spectrometry (MS)-based proteins metabolites a single cell lagged until recently. Undoubtedly, MS-based will provide deeper insight into mechanisms related health disease. review summarizes recent advances methods their applications biology medicine.

Language: Английский

Citations

21