Vertical Silica Nanochannels and o-Phenanthroline Chelator for the Detection of Trace Fe(II)

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(7), P. 7743 - 7752

Published: March 20, 2024

Herein, we develop a simple electrochemical sensor based on the confinement effect of vertically ordered mesoporous silica film (VMSF) and o-phenanthroline (Phen) chelating agent for ultrasensitive determination ferrous ions (Fe2+). Fe(Phen)32+ obtained from chelation between Fe2+ Phen, shows higher activity larger geometric dimensions compared with free Fe2+, which could be effectively preconcentrated into inner nanochannels VMSF through spatial electrostatic effects. Differing conventional anodic stripping voltammetry, enriched by convenient mechanical stirring further detected VMSF-modified indium tin oxide (VMSF/ITO), achieving an outstanding detection performance in terms linear range (1.0 nM–13.0 μM) limit (LOD, 0.66 nM). Moreover, established can accurately measure total iron distinguish different valence states (Fe2+ or Fe3+) help reducing agents. Thanks to antifouling ability VMSF, sensing platform consisting VMSF/ITO Phen enables precise complex real samples including tap water, pond FeSO4 tablets, even colored samples, avoid tedious sample pretreatment process applied wide scenarios.

Language: Английский

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Solid Electrochemiluminescence Sensor by Immobilization of Emitter Ruthenium(II)tris(bipyridine) in Bipolar Silica Nanochannel Film for Sensitive Detection of Oxalate in Serum and Urine

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(5), P. 390 - 390

Published: Feb. 20, 2024

Convenient and highly sensitive detection of oxalate ions in body fluids is crucial significance for disease prevention, diagnosis, monitoring treatment effectiveness. Establishing a simple solid-state electrochemiluminescence (ECL) sensing system desirable. In this work, solid ECL sensor was fabricated by immobilizing the commonly used emitter ruthenium(II)tris(bipyridine) (Ru(bpy)32+) on double-layered bipolar silica nanochannel array film (bp-SNA)-modified electrode, enabling serum or urine samples. Cost-effective readily available indium tin oxide (ITO) as supporting electrode. fabrication multiple negatively charged SNA (n-SNA)-modified ITO electrodes achieved through one-step Stöber solution growth method. Subsequently, positive outer layer (p-SNA) rapidly prepared using an electrochemical-assisted self-assembly The stable immobilization Ru(bpy)32+ electrode surface, facilitated electrostatic adsorption n-SNA repulsion p-SNA. Utilizing co-reactant Ru(bpy)32+, combined with enrichment p-SNA, constructed enabled ranging from 1 nM to 25 μM mM, limit (LOD) 0.8 nM. exhibited high selectivity good stability, making it suitable

Language: Английский

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Nanogold amplified electrochemiluminescence/electrochemistry in bipolar silica nanochannel array for ultrasensitive detection of SARS-CoV-2 pseudoviruses

Talanta, Journal Year: 2024, Volume and Issue: 277, P. 126319 - 126319

Published: May 27, 2024

Language: Английский

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Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 63 - 63

Published: Jan. 19, 2025

The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach fast and convenient biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains challenge. This study developed an immunosensor by engineering immunorecognition interface on outer surface mesoporous silica nanochannel film (SNF) confining Co3O4 nanocatalyst within SNF nanochannels to improve luminol efficiency. was grown indium tin oxide (ITO) electrode using simple Stöber solution growth method. A successfully confined through situ electrodeposition, confirmed X-ray photoelectron spectroscopy (XPS) electrochemical measurements. demonstrated excellent electrocatalytic activity, effectively enhancing H2O2 oxidation boosting signal conditions. Using interleukin-6 (IL-6) as proof-of-concept demonstration, epoxy functionalization enabled covalent immobilization capture antibodies, forming specific interface. IL-6 binding induced immunocomplex formation, which reduced allowed quantitative detection. showed linear range from 1 fg mL−1 10 ng mL−1, with limit (LOD) 0.64 mL−1. It also good selectivity anti-interference capabilities, enabling successful artificial GCF samples.

Language: Английский

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Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol

Biosensors, Journal Year: 2024, Volume and Issue: 14(8), P. 403 - 403

Published: Aug. 21, 2024

The convenient and sensitive detection of metabolites is great significance for understanding human health status drug development. Solid-phase electrochemiluminescence (ECL) enzyme electrodes show potential in metabolite based on the enzyme-catalyzed reaction product hydrogen peroxide (H2O2). Herein, a solid-phase ECL sensor was fabricated confined emitter an immobilized using electrostatic nanocage array, constructing platform cholesterol. cage nanochannel consists bipolar bilayer vertically aligned mesoporous silica film (bp-VMSF). upper layer bp-VMSF amino-modified, positively charged VMSF (p-VMSF), lower negatively (n-VMSF). most commonly used probe tris(bipyridine)ruthenium(II) (Ru(bpy)32+) fixed n-VMSF by adsorption from repulsion p-VMSF, generating significantly enhanced stable signals. successful preparation characterized scanning electron microscopy (SEM) electrochemical methods. After amino groups outer surface were derivatized with aldehyde, cholesterol oxidase (ChOx) molecules covalently immobilized. construction electrode cyclic voltammetry (CV) impedance spectroscopy (EIS). When corresponding substrate, cholesterol, present solution, signal Ru(bpy)32+ quenched H2O2, enabling high-sensitivity linear range detecting 0.05 mM to 5.0 mM, limit (LOD) 1.5 μM.

Language: Английский

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Electrochemical/Electrochemiluminescence Sensors Based on Vertically‐Ordered Mesoporous Silica Films for Biomedical Analytical Applications

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: June 14, 2024

Abstract Vertically‐ordered mesoporous silica films (VMSF, also named as isoporous membranes) have shown tremendous potential in the field of electroanalytical sensors due to their unique features terms controllable and ultrasmall nanopores, high molecular selectivity permeability, mechanical stability. This review will present recent progress on biomedical analytical applications VMSF, focusing small biomolecules, diseases‐related biomarkers, drugs cancer cells. Finally, conclusions with developments future perspective VMSF relevant fields be envisioned.

Language: Английский

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Simple fabrication of electrochemical sensor based on integration of dual signal amplification by the supporting electrode and modified nanochannel array for direct and sensitive detection of vitamin B2

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: March 15, 2024

Development of simple and reliable sensor for detecting vitamin content is great significance guiding human nutrition metabolism, overseeing the quality food or drugs, advancing treatment related diseases. In this work, a electrochemical was conveniently fabricated by modification carbon electrode with vertically-ordered mesoporous silica film (VMSF), enabling highly sensitive detection B 2 (VB ) based on dual enrichment analyte supporting nanochannels. The widely used glassy (GCE), preactivated using polarization, resulting GCE (p-GCE) exhibited improved potential resolution ability enhanced peak current VB . Stable VMSF p-GCE (VMSF/p-GCE) achieved without introducing another binding layer. effect nanochannels facilitated , linear concentration ranged from 20 nM to 7 μM μM. limit (LOD), determined signal-to-noise ratio three (S/N = 3), found be 11 nM. ultra-small endowed VMSF/p-GCE excellent anti-interference anti-fouling performance, along high stability. constructed demonstrated capability achieve direct in turbid samples including milk leachate compound tablet need complex sample pretreatment. can easily regenerated has reusability. advantages preparation, good regeneration endow small molecule samples.

Language: Английский

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Mesoporous carbon nanospheres-assisted amplified electrochemiluminescence for L-cysteine detection

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 115764 - 115764

Published: Jan. 1, 2025

Language: Английский

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Highly Sensitive Electrochemical Determination of Butylated Hydroxyanisole in Food Samples Using Electrochemical-Pretreated Three-Dimensional Graphene Electrode Modified with Silica Nanochannel Film

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(7), P. 569 - 569

Published: March 25, 2024

The sensitive detection of antioxidants in food is essential for the rational control their usage and reducing potential health risks. A simple three-dimensional (3D) electrode integrated with an anti-fouling/anti-interference layer possesses great direct electrochemical samples. In this work, a 3D sensor was developed by integrating graphene (3DG) vertically ordered mesoporous silica film (VMSF), enabling highly common antioxidant, butylated hydroxyanisole (BHA), polarization employed to pre-activate 3DG (p3DG), enhancing its hydrophilicity. Using p3DG as supporting electrode, stable modification VMSF achieved using assisted self-assembly (EASA) method, without need any adhesive agents (VMSF/p3DG). Taking BHA model analyte, VMSF/p3DG demonstrated high sensitivity, due enrichment nanochannels, towards BHA. Electrochemical linear range 0.1 μM 5 from 150 low limit (12 nM). Owing fouling resistance anti-interference capabilities VMSF, constructed can be directly applied complex

Language: Английский

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Nanochannel-confined platinum nanostructure for enhancement of luminol-dissolved oxygen electrochemiluminescence coupled with gated aptasensor for sensitive detection of carcinoembryonic antigen

Microchemical Journal, Journal Year: 2024, Volume and Issue: 206, P. 111413 - 111413

Published: Aug. 12, 2024

Language: Английский

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