Composition and diversity of gut microbiota in diabetic retinopathy

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 23, 2022

Diabetic retinopathy (DR) is one of the most common complications type 2 diabetes mellitus. The current study investigates composition, structure, and function gut microbiota in DR patients explores correlation between clinical characteristics DR.A total 50 stool samples were collected from participants, including 25 healthy controls (HCs). 16S ribosomal RNA gene sequencing was used to analyze microbial composition these two groups. DNA extracted fecal using MiSeq platform.The structure different that HCs. richness higher than normal individuals. alterations microbiome associated with disrupted Firmicutes, Bacteroidetes, Synergistota, Desulfobacterota phyla. In addition, increased levels Bacteroides, Megamonas, Ruminococcus_torques_group, Lachnoclostridium, Alistipes, decreased Blautia, Eubacterium_ hallii_group, Collinsella, Dorea, Romboutsia, Anaerostipes, Fusicatenibacter genera observed Additionally, a stochastic forest model developed identify set biomarkers seven bacterial can differentiate those HC. communities exhibited varied functions groups because above-mentioned genera.The altered indicated could be as non-invasive biomarkers, improve diagnostic methods, putative therapeutic targets for DR.

Language: Английский

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Structural characterization and immunomodulatory activity of a polysaccharide from Dioscotea opposita

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130734 - 130734

Published: March 8, 2024

Language: Английский

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Stress and depression-associated shifts in gut microbiota: A pilot study of human pregnancy

Brain Behavior & Immunity - Health, Journal Year: 2024, Volume and Issue: 36, P. 100730 - 100730

Published: Jan. 26, 2024

Psychosocial stress and mood-related disorders, such as depression, are prevalent vulnerability to these conditions is heightened during pregnancy. induces consequences via several mechanisms including the gut microbiota-brain axis associated signaling pathways. Previous preclinical work indicates that prenatal alters maternal microbial composition impairs offspring development. Importantly, although fecal vaginal microenvironments undergo alterations across pregnancy, we lack consensus regarding which shifts adaptive or maladaptive in presence of depression. Clinical studies interrogating relationships have identified unique taxa but been limited study design.

Language: Английский

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Strategies adopted by Salmonella to survive in host: a review

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(12)

Published: Oct. 31, 2023

Language: Английский

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Type-2 Diabetes Mellitus and the Gut Microbiota: Systematic Review

Cureus, Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 30, 2023

The gut microbiota is a community situated in the gastrointestinal tract that consists of bacteria thriving and contributing to functions our body. It heavily influenced by what individuals eat, as quality, amount, frequency food consumed can favor inhibit specific bacteria. Type-2 diabetes mellitus (T2DM) common but detrimental condition arises from excessive hyperglycemia, leading either insulin resistance or damage B-cells produce pancreas. A poor diet high sugar fats leads this persists, it lead development T2DM. Both are greatly affected an individual consumes, there more involved relationship between T2DM? This paper aimed evaluate changes patients with T2DM impacts microbiota. Bacteroides, Proteobacteria, Firmicutes, Actinobacteria prevailed healthy control, their abundance varied greatly. There was also significant decrease like Lactobacilli spp. F. prausnitizii associated resistance. High levels BMI have been increased A. muciniphilia, which has decreased fat metabolism BMI. Metabolites such butyrates melatonin identified influencing progression Testosterone T2DM, males lower testosterone greater Gemella, Lachnospiraceae, Massiia. Identifying these how they impact body may treatment addressing dysfunction altered to. Future research should address methods diets, exercise, anti-diabetics affect see if influence sustained reduced hyperglycemia.

Language: Английский

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Gut microbiota composition is altered in postural orthostatic tachycardia syndrome and post-acute COVID-19 syndrome

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 9, 2024

Abstract Postural Orthostatic Tachycardia Syndrome (POTS) reflects an autonomic dysfunction, which can occur as a complication to COVID-19. Our aim was examine gastrointestinal symptoms and gut microbiota composition in patients with POTS post-acute COVID-19 syndrome (PACS), compared controls. ( n = 27), PACS 32) controls 39) delivered fecal samples completed 4-day food diary, irritable bowel syndrome-severity scoring system (IBS-SSS), visual analog scale for IBS (VAS-IBS). A total of 98 DNA aliquots were sequenced average depth 28.3 million (M) read pairs (Illumina 2 × 150 PE) per sample. Diversity taxonomic levels the microbiome, well functional abundances calculated groups, then There several differences between controls, whereas only abundance Ascomycota Firmicutes differed The clinical variables IBS-SSS, fatigue, bloating flatulence significantly correlated multiple individual taxa abundances, alpha diversity, abundances. We conclude that POTS, less extent PACS, are associated diversity at levels. Clinical both

Language: Английский

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Yi-Shen-Hua-Shi regulates intestinal microbiota dysbiosis and protects against proteinuria in patients with chronic kidney disease: a randomized controlled study

Pharmaceutical Biology, Journal Year: 2024, Volume and Issue: 62(1), P. 356 - 366

Published: May 9, 2024

Context Yi-Shen-Hua-Shi (YSHS) is a traditional Chinese medicine that treats chronic kidney disease (CKD). However, its efficacy in reducing proteinuria and underlying mechanisms unknown.

Language: Английский

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Integrating 16S rDNA and metabolomics to uncover the therapeutic mechanism of electroacupuncture in type 2 diabetic rats

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 6, 2025

This study aimed to investigate the impact of electroacupuncture (EA) on blood glucose levels, gut microbiota, short-chain fatty acids (SCFAs), and glucagon-like peptide-1 (GLP-1) in a rat model type 2 diabetes mellitus (T2DM). Forty Sprague-Dawley (SD) rats were randomly assigned five groups (n = 8/group) using random number table: normal control, T2DM model, (EA), EA + antibiotics (EA A), (A). The received standard diet saline gavage, while other fed high-fat emulsion. A additional antibiotic solution gavage. normal, immobilized restrained for 30 min, six times per week, 4 weeks. treatment at specific acupoints parameters continuous waves 10 Hz 1-2 mA. During intervention, water food consumption, body weight, fasting (FBG), oral tolerance test (OGTT) monitored. Pancreatic tissue was examined hematoxylin eosin (H&E) staining. Fecal microbial communities analyzed by 16S rDNA sequencing, (SCFAs) measured gas chromatography-mass spectrometry (GC-MS). Serum levels insulin (FINS), glycated hemoglobin (HbA1c), determined enzyme-linked immunosorbent assay (ELISA). significantly improved daily intake, weight (p < 0.01). also reduced FBG, area under curve OGTT, FINS, homeostasis assessment resistance (HOMA-IR) 0.05). ELISA results showed lower concentration HbA1c group overall morphology pancreatic islets, increased β-cell nuclei, alleviated hypertrophy. abundance operational taxonomic units (OTUs) than 0.05), upregulated Shannon, Chao1, Ace indices concentrations acetic acid, butyric GLP-1 Correlation analysis revealed negative associations between Lactobacillaceae (R -0.81, p 0.015) Lactobacillus -0.759, 0.029) with FBG. Peptostreptococcaceae Romboutsia negatively correlated 0.015), Enterobacteriaceae positively OGTT 0.762, 0.028). acid 0.487, 0.001), 0.586, 0.000), isovaleric 0.374, 0.017), valeric 0.535, caproic 0.371, 0.018). Antibiotics disrupted intestinal microbiota structure weakened therapeutic effects EA. effectively metabolism rats. hypoglycemic associated regulation SCFAs, GLP-1.

Language: Английский

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A systematic review on gut microbiota in type 2 diabetes mellitus

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 17, 2025

The gut microbiota play crucial roles in the digestion and degradation of nutrients, synthesis biological agents, development immune system, maintenance gastrointestinal integrity. Gut dysbiosis is thought to be associated with type 2 diabetes mellitus (T2DM), one world's fastest growing diseases. aim this systematic review identify differences composition diversity individuals T2DM. A search was conducted studies reporting on difference between T2DM healthy controls. Relevant were evaluated, their characteristics results extracted using a standardized data extraction form. assessed for risk bias findings reported narratively. 58 observational published 2010 2024 included. Beta commonly different individuals. Genera Lactobacillus, Escherichia-Shigella, Enterococcus, Subdoligranulum Fusobacteria found positively associated; while Akkermansia, Bifidobacterium, Bacteroides, Roseburia, Faecalibacteirum Prevotella negatively This demonstrates strong association dysbiosis, as evidenced by differential microbial abundances altered indices. Among these taxa, Escherichia-Shigella consistently T2DM, whereas Faecalibacterium prausnitzii appears offer protective effect against However, heterogeneity nature preclude establishment causative relationships. Future research should incorporate age, diet medication-matched controls, include functional analysis microbes. https://www.crd.york.ac.uk/prospero/, identifier CRD42023459937.

Language: Английский

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Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats

Journal of Neuroinflammation, Journal Year: 2025, Volume and Issue: 22(1)

Published: Feb. 2, 2025

Language: Английский

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