Tracking tripartite interaction dynamics: isolation, integration, and influence of bacteriophages in the Paraburkholderia-Dictyostelium discoideum symbiosis system DOI Creative Commons
Susanne DiSalvo, Nicholas J. Maness, Andrew P. Braun

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 2, 2025

Introduction Bacteriophages influence interactions between bacterial symbionts and their hosts by exerting parasitic pressure on symbiont populations facilitating evolution through selection, gene exchange, prophage integration. Host organisms also modulate phage-bacteria interactions, with host-specific contexts potentially limiting or promoting phage access to driving alternative phenotypic evolutionary outcomes. Methods To better elucidate tripartite phage-bacteria-host in real-time, we expanded the Dictyostelium discoideum-Paraburkholderia symbiosis system include Paraburkholderia -specific phages. We isolated six environmental phages from soil samples using a multi-host enrichment approach. identified functional monocultures of one strains implemented These were evaluated across all three amoeba-associated species. Finally, treated infected amoeba lines select isolates assessed effects prevalence host fitness. Results The exhibited diverse plaquing characteristics virion morphologies, collectively targeting belonging each amoeba-symbiotic Following treatment experiments, observed that application some cases reduced infection alleviated fitness impacts, while others, no significant noted. Notably, able persist within symbiont-infected over multiple culture transfers, indicating potential long-term interactions. Discussion findings highlight variability phage-symbiont environment underscore complex nature lays foundation for future studies exploring dynamics systems, suggesting mechanisms may shape differential outcomes presenting valuable avenues investigation.

Language: Английский

The gut microbiota and its biogeography DOI
Giselle McCallum, Carolina Tropini

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 22(2), P. 105 - 118

Published: Sept. 22, 2023

Language: Английский

Citations

134
Fecal microbiota transplantation: current challenges and future landscapes DOI
Abbas Yadegar, Haggai Bar‐Yoseph, Tanya Monaghan

et al.

Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: 37(2)

Published: May 8, 2024

SUMMARYGiven the importance of gut microbial homeostasis in maintaining health, there has been considerable interest developing innovative therapeutic strategies for restoring microbiota. One such approach, fecal microbiota transplantation (FMT), is main "whole microbiome replacement" strategy and integrated into clinical practice guidelines treating recurrent

Language: Английский

Citations

45
Diabetes and gut microbiome DOI Creative Commons
K. Fliegerová, Tiziana Maria Mahayri, Hana Sechovcová

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Diabetes mellitus represents a significant global health problem. The number of people suffering from this metabolic disease is constantly rising and although the incidence heterogeneous depending on region, country, economic situation, lifestyle, diet level medical care, it increasing worldwide, especially among youths children, mainly due to lifestyle environmental changes. pathogenesis two most common subtypes diabetes mellitus, type 1 (T1DM) 2 (T2DM), substantially different, so each form characterized by different causation, etiology, pathophysiology, presentation, treatment. Research in recent decades increasingly indicates potential role gut microbiome initiation, development, progression disease. Intestinal microbes their fermentation products have an important impact host metabolism, immune system, nutrient digestion absorption, barrier integrity protection against pathogens. This review summarizes current evidence changes microbial populations both types mellitus. Attention focused abundance specific bacterial groups at taxonomic levels humans, shift also assessed relation geographic location, age, antidiabetic drug. causal relationship between bacteria still unclear, future studies applying new methodological approaches broader range microorganisms inhabiting digestive tract are urgently needed. would not only provide better understanding disease, but use beneficial species probiotics for treatment diabetes.

Language: Английский

Citations

2
The frontier of health: Exploring therapeutic potentials of the microbiome DOI

Mohammad Abavisani,

Sobhan Karbas Foroushan, Prashant Kesharwani

et al.

PharmaNutrition, Journal Year: 2025, Volume and Issue: unknown, P. 100435 - 100435

Published: Feb. 1, 2025

Language: Английский

Citations

2
Signals triggering prophage induction in the gut microbiota DOI

Caroline Henrot,

Marie‐Agnès Petit

Molecular Microbiology, Journal Year: 2022, Volume and Issue: 118(5), P. 494 - 502

Published: Sept. 27, 2022

Compared to bacteria of the gut microbiota, bacteriophages are still poorly characterised, and their physiological importance is far less known. Temperate phages probably a major actor in gut, as it estimated that 80% intestinal lysogens, meaning they carrying prophages. In addition, prophage induction rates higher than vitro. However, studies on signals leading have essentially focused genotoxic agents with poor relevance for this environment. review, we sum up recent findings about able trigger gut. Three categories at play: those originating from interactions between microbes, human or animal host physiology external intakes. These results highlight diversity factors influencing start unveil ways by which microbiota composition may be modulated.

Language: Английский

Citations

39
Bacteriophages and the Microbiome in Dermatology: The Role of the Phageome and a Potential Therapeutic Strategy DOI Open Access
Nicole Natarelli,

Nimrit Gahoonia,

Raja K. Sivamani

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2695 - 2695

Published: Jan. 31, 2023

Bacteriophages, also known as phages, are viruses that selectively target and infect bacteria. In addition to bacterial dysbiosis, dermatologic conditions such acne, psoriasis, atopic dermatitis characterized by a relative reduction in the abundance of phages overgrowth corresponding Phages often exhibit high specificity for their targeted bacteria, making phage-replacement therapy promising therapeutic strategy control pathogenic bacteria disease. Novel strategies regulating especially necessary light growing antibiotic resistance. this review, we aimed review medical literature assessing phage dysbiosis trials dermatology. Ultimately, studies have depicted results treatment but limited low sample sizes omission groups some trials. Additional work is validate efficacy proof-of-concept further determine optimal vehicles, administration mechanisms, dosing schedules. This provides framework assessment future

Language: Английский

Citations

32
An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns DOI Creative Commons
Amr Abd El‐Wahab, Shereen Basiouni, Hesham R. El‐Seedi

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 21, 2023

The primary contaminants in poultry are Salmonella enterica , Campylobacter j ejun i, Escherichia coli, and Staphylococcus aureus . Their pathogenicity together with the widespread of these bacteria, contributes to many economic losses poses a threat public health. With increasing prevalence bacterial pathogens being resistant most conventional antibiotics, scientists have rekindled interest using bacteriophages as antimicrobial agents. Bacteriophage treatments also been investigated an alternative antibiotics industry. Bacteriophages’ high specificity may allow them only target specific pathogen infected animal. However, tailor-made sophisticated cocktail different could broaden their antibacterial activity typical situations multiple clinical strains infections. Bacteriophages not be used terms reducing contamination animals but also, under industrial conditions, they can safe disinfectants reduce on food-contact surfaces or carcasses. Nevertheless, bacteriophage therapies developed sufficiently for use. Problems resistance, safety, specificity, long-term stability must addressed particular. This review highlights benefits, challenges, current limitations applications

Language: Английский

Citations

28
Phage-microbe dynamics after sterile faecal filtrate transplantation in individuals with metabolic syndrome: a double-blind, randomised, placebo-controlled clinical trial assessing efficacy and safety DOI Creative Commons
Koen Wortelboer, Patrick A. de Jonge, Torsten P. M. Scheithauer

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 12, 2023

Bacteriophages (phages) are bacterial viruses that have been shown to shape microbial communities. Previous studies faecal virome transplantation can decrease weight gain and normalize blood glucose tolerance in diet-induced obese mice. Therefore, we performed a double-blind, randomised, placebo-controlled pilot study which 24 individuals with metabolic syndrome were randomised filtrate (FFT) from lean healthy donor (n = 12) or placebo 12). The primary outcome, change metabolism, secondary outcomes, safety longitudinal changes within the intestinal bacteriome phageome, assessed baseline up 28 days. All included subjects completed analyses. While overall metabolism not significantly different between both groups, FFT is well-tolerated without any serious adverse events. phage virion composition altered two days after as compared placebo, coincides more virulent phage-microbe interactions. In conclusion, provide evidence gut phages be safely administered transiently alter microbiota of recipients.

Language: Английский

Citations

25
Analysis and culturing of the prototypic crAssphage reveals a phage-plasmid lifestyle DOI Creative Commons
Danica T. Schmidtke, Angela Shang-Mei Hickey, Ivan Liachko

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Summary The prototypic crAssphage ( Carjivirus communis ) is one of the most abundant, prevalent, and persistent gut bacteriophages, yet it remains uncultured its lifestyle uncharacterized. For last decade, has escaped plaque-dependent culturing efforts, leading us to investigate alternative lifestyles that might explain widespread success. Through genomic analyses culturing, we find uses a phage-plasmid persist extrachromosomally. Plasmid-related genes are more highly expressed than those implicated in phage maintenance. Leveraging this finding, use plaque-free approach measure replication culture with Phocaeicola vulgatus, dorei, Bacteroides stercoris , revealing broad host range. We demonstrate persists hosts without causing major cell lysis events or integrating into chromosomes. ability switch between plasmid within wide range contributes prolific nature human microbiome.

Language: Английский

Citations

13
Integrative metagenomic analysis reveals distinct gut microbial signatures related to obesity DOI Creative Commons

Xinliang Hu,

Yu Chong, Yuting He

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: April 5, 2024

Abstract Obesity is a metabolic disorder closely associated with profound alterations in gut microbial composition. However, the dynamics of species composition and functional changes microbiome obesity remain to be comprehensively investigated. In this study, we conducted meta-analysis metagenomic sequencing data from both obese non-obese individuals across multiple cohorts, totaling 1351 fecal metagenomes. Our results demonstrate significant decrease richness diversity bacteriome virome patients. We identified 38 bacterial including Eubacterium sp. CAG:274, Ruminococcus gnavus , eligens Akkermansia muciniphila, 1 archaeal species, Methanobrevibacter smithii that were significantly altered obesity. Additionally, observed abundance five viral families: Mesyanzhinovviridae Chaseviridae Salasmaviridae Drexlerviridae Casjensviridae . Functional analysis indicated distinct signatures as primary driver for function enrichment obesity, muciniphila bicirculans siraeum drivers healthy control group. our suggest antibiotic resistance genes virulence factors may influence development Finally, demonstrated vOTUs achieved diagnostic accuracy an optimal area under curve 0.766 distinguishing controls. findings offer comprehensive generalizable insights into features potential guide microbiome-based diagnostics.

Language: Английский

Citations

10