Detection of recombinant Spike protein in the blood of individuals vaccinated against SARS‐CoV‐2: Possible molecular mechanisms

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2023, Volume and Issue: 17(6)

Published: Aug. 31, 2023

Abstract Purpose The SARS‐CoV‐2 pandemic prompted the development and use of next‐generation vaccines. Among these, mRNA‐based vaccines consist injectable solutions mRNA encoding for a recombinant Spike, which is distinguishable from wild‐type protein due to specific amino acid variations introduced maintain in prefused state. This work presents proteomic approach reveal presence Spike vaccinated subjects regardless antibody titer. Experimental design Mass spectrometry examination biological samples was used detect fragments who received Results PP‐Spike fragment found 50% analyzed, its independent IgG minimum maximum time at detected after vaccination 69 187 days, respectively. Conclusions clinical relevance presented method allows evaluate half‐life molecule “PP” consider risks or benefits continuing administer additional booster doses vaccine. valuable support complement level monitoring represents first detection subjects.

Language: Английский

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The profile of blood microbiome in new-onset type 1 diabetes children

iScience, Journal Year: 2024, Volume and Issue: 27(7), P. 110252 - 110252

Published: June 13, 2024

Blood microbiome signatures in patients with type 1 diabetes (T1D) remain unclear. We profile blood using 16S rRNA gene sequencing 77 controls and 64 children new-onset T1D, compared it the gut oral microbiomes. The of T1D is characterized by increased diversity perturbed microbial features, a significant increase potentially pathogenic bacteria controls. Thirty-six representative genera were identified random forest analysis, providing strong discriminatory power for an AUC 0.82. PICRUSt analysis suggested that capable inducing inflammation more likely to enter bloodstream T1D. overlap implied potential translocation from cavity bloodstream. Our study raised necessity further mechanistic investigations into roles

Language: Английский

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The Diagnostic Potential of the Human Blood Microbiome: Are We Dreaming or Awake?

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(13), P. 10422 - 10422

Published: June 21, 2023

Human blood has historically been considered a sterile environment. Recently, thriving microbiome dominated by Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes phyla was detected in healthy blood. The localization of these microbes is restricted to some cell populations, particularly the peripheral mononuclear cells erythrocytes. It hypothesized that originates from skin-oral-gut axis. In addition, many studies have evaluated potential dysbiosis as prognostic marker cardiovascular diseases, cirrhosis, severe liver fibrosis, acute pancreatitis, type 2 diabetes, chronic kidney diseases. present review aims summarize current findings most recent evidence field.

Language: Английский

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The Bacterial and pathogenic landscape of African buffalo (Syncerus caffer) whole blood and serum from Kenya

Animal Microbiome, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 12, 2025

African buffalo (Syncerus caffer) is a significant reservoir host for many zoonotic and parasitic infections in Africa. These include range of viruses pathogenic bacteria, such as tick-borne rickettsial organisms. Despite the considerations mammalian blood sterile environment, microbiome sequencing could become crucial agnostic biosurveillance. This study investigated clinically healthy wild buffaloes Kenya to determine its applicability testing bacteria apparently animals. Whole serum samples were collected from 46 buffalos Meru National Park (30), Buffalo Springs (6) Shaba (10) Reserves upper eastern Kenya. Total deoxyribonucleic acid (DNA) was extracted these subjected amplicon-based targeting 16 S rRNA gene. The operational taxonomic units (OTU) identified species levels by mapping generated V12 V45 regions gene SILVA database. OTU tables used infer microbial abundance each sample type at individual animal level. sequences corresponding OTUs also generate phylogenetic trees thus evolution interest. Here, we demonstrate that harbor their blood. We report diversity Anaplasma Mycoplasma By both whole triplicate animal, provide evidence differences detecting types. Diverse including some potential pathogens, can be found buffalo. Agnostic surveillance pathogens achieved through sequencing. However, question being asked wildlife will impact choice using or

Language: Английский

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Blood microbiome and cardiometabolic disease: insights, therapeutic strategies, and future directions

Frontiers in Bacteriology, Journal Year: 2025, Volume and Issue: 4

Published: March 3, 2025

Cardiometabolic diseases (CMDs), particularly cardiovascular disease (CVD), type 2 diabetes mellitus (T2DM), and chronic kidney (CKD), emerged as primary contributors to global morbidity mortality. In addition traditional factors, recent studies demonstrated that blood microbiomes may also promote the development or progression of these CMDs. Traditionally, was considered sterile; however, notion a sterile environment has been challenged by findings demonstrating presence microbiome in both healthy states. Although there tremendous expansion human research, with hundreds projects underway globally not received same level attention its gut oral counterparts. The circulating is an emerging trend drawn high interest biomedical field, given potential generate predictive biomarkers means screen for pathogens. This comprehensive review explores latest advancements emphasizing biomarker identification, diagnostic tools, treatment modalities, prevention We delve into existing challenges present future-oriented strategy using advanced methods. Deciphering microbiome’s role could lead classification patient subgroups, enabling precision microbiota-based therapies.

Language: Английский

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Blood Bacterial Microbiota of the American Bison (Bison bison) in Northern Mexico: A Reference for Health and Conservation

Ruminants, Journal Year: 2025, Volume and Issue: 5(1), P. 10 - 10

Published: March 4, 2025

The emerging field of study blood microbiota reveals the presence bacteria in healthy animals. In endangered species such as American bison (Bison bison), analysis this is crucial for conservation, changes these communities or development pathogens may affect their health and compromise herd viability. Here, we analyzed compared bacterial adult juvenile Mexico (Janos, Chihuahua), identifying those taxa with potential pathogenicity individuals. Blood samples were collected from 12 bison. V3–V4 region 16S rRNA gene was amplified, next-generation sequencing subsequently performed on Illumina NovaSeq platform. observed individuals (Firmicutes, Proteobacteria, Bacteroidota, Actinobacteria, Fusobacteriaceae, Lachnospiraceae, Oscillospiraceae, Ruminococcaceae) have been previously reported to be abundant rumen feces most notable difference Mycoplasma wenyonii, which significantly enriched juveniles adults. New technologies can practically applied improve management conservation vulnerable

Language: Английский

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Isolation and Characterization of the Microbiota from the Left Ventricle in NIH Mice (Mus musculus): An In Vitro Study

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Aim: The aim of this study was to characterize the hematic microbiota left ventricle NIH strain mice via culture, Gram staining and catalase assays. Materials Methods: An in vitro conducted Department Pharmacology Faculty Medicine at Autonomous University Chiapas Laboratory Experimental Microbiology Technological Institute Tuxtla Gutierrez, Mexico, between November 2019 February 2020. Thirty were used, distributed into groups maintained under sterile conditions. Blood samples obtained puncture inoculated different culture media (blood agar, Sabouraud agar brain-heart agar) for isolation characterization microbiota. Results: results revealed growth microorganisms on all media. On blood 22, 22 29 colony-forming units (CFUs) recorded A, B C, respectively. 65, 44 78 CFUs observed, 26, 131 10 observed. Taxonomic presence gram-positive gram-negative bacteria, as well fungi, with a predominance Streptococcus Bacillus samples. Conclusion: This existence native mice, which are predominantly gram-negative, diverse fungi.

Language: Английский

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Tracking the origin of bacterial DNA in blood: Indication of localized and sporadic transfer from other body sites

Gut Microbes Reports, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: April 1, 2025

Language: Английский

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Insights into the blood, gut, and oral microbiomes in Chinese patients with myocardial infarction: a case-control study

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 19, 2025

Emerging evidence suggests that changes in the blood microbes might be associated with cardiovascular disease, especially myocardial infarction (MI). However, some researchers are questioning whether a true "blood microbiome" actually exists. They hypothesized these may translocate into bloodstream from gut or oral cavities. To test this hypothesis, we analyzed microbial composition, diversity, and potential role disease progression by comparing blood, gut, microbiota profiles cohort of MI patients healthy controls. In study, 144 samples, including fecal, saliva, were collected twenty-four These samples using 16 S rRNA sequencing to characterize across three distinct compartments. Differential analyses conducted find key differential for MI. Spearman's rank correlation analysis was used study association between clinical indicators. Our findings revealed striking shifts compartments compared observed significant enrichment phyla Armatimonadota Caldatribacteriota, alongside genera Bacillus, Pedobacter, Odoribacter. The showed notable increase Proteobacteria, Verrucomicrobiota, Cyanobacteria, Synergistota, Crenarchaeota, as well Eubacterium_coprostanoligenes_group, Rothia, Akkermansia, Lachnospiraceae_ NK4A136_ group, Eubacterium_ruminantium_group. Meanwhile, uniquely enriched phylum Elusimicrobiota Streptococcus, Granulicatella. signatures highlight compartment-specific alterations play pathophysiology Additionally, LEfSe identified 64 taxa differed Of these, eight unique eighteen thirty-eight microbiota, all which demonstrated associations markers Functional pathways predicted via KEGG annotation, but no statistically differences found controls any microbiome This demonstrates patients, identifying specific bacterial strongly infarction. patterns detected provide compelling existence stable core microbiome, highlighting its importance contributor health progression.

Language: Английский

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Microbiome and fragmentation pattern of blood cell-free DNA and fecal metagenome enhance colorectal cancer micro-dysbiosis and diagnosis analysis: a proof-of-concept study

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT Colorectal cancer (CRC) is the third most common cancer, and it can be prevented by performing early screening. As a hallmark of human microbiome plays important roles in occurrence development CRC. Recently, blood has been proposed as an effective diagnostic tool for various diseases, yet its performance on CRC deserves further exploration. In this study, 133 feces 120 samples are collected, including healthy individuals, adenoma patients, patients. The cfDNA fecal genome subjected to shotgun metagenome sequencing. After removing sequences, microbial sequences analyzed. Based differential microbes functions, random forest (RF) models constructed diagnosis. results show that alterations signatures captured under low coverage (even at 3×). RF based markers achieve high area curve (AUC) values patients (0.8849) (0.9824). When fragmentation pattern combined with KEGG markers, higher AUC obtained. Furthermore, compared microbiome, shows different community composition, whereas their changes pathways similar. Pathogenic bacteria Fusobacterium nucleatum ( F. ) increased gradually from group groups. Additionally, positive correlation Cumulatively, integration promising IMPORTANCE cell-free DNA enter used diagnosis, potential colorectal association gut not explored. account less than 1% total sequences. among Machine learning these characteristics accuracy, especially when they integrated patterns. great difference between microbiomes indicates may originate organs. Therefore, study provides new insights into composition functions proposes non-invasive tool.

Language: Английский

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