Springer series on biofilms, Journal Year: 2024, Volume and Issue: unknown, P. 259 - 282
Published: Jan. 1, 2024
Language: Английский
Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(5)
Published: Oct. 1, 2024
Abstract Vertebrate embryos are protected from bacterial infection by various maternally derived factors, yet little is known about the defence mechanisms in elasmobranchs. This study aimed to characterize intracapsular environment of freshly laid eggs oviparous catshark ( Scyliorhinus torazame ) investigating microbial abundance and microbiota understand its potential contribution embryonic defence. The egg capsule elasmobranchs tightly sealed until pre‐hatching, after which seawater flows into capsule, exposing surrounding seawater. We found that early were highly vulnerable environmental pathogens, suggesting somehow before pre‐hatching. Indeed, exhibited significantly low density, maintained Furthermore, microbiome inside just oviposition differed markedly those rearing adult oviducal gland epithelia; these predominantly populated an unidentified genus Sphingomonadaceae. Overall, this provides compelling evidence cloudy incubated a clean potentially plays significant role development
Language: Английский
Citations
0
Solid State Nuclear Magnetic Resonance, Journal Year: 2024, Volume and Issue: 134, P. 101975 - 101975
Published: Oct. 25, 2024
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 25, 2024
ABSTRACT Pseudomonas aeruginosa frequently causes chronic lung infection in individuals with muco-obstructive airway diseases (MADs). Chronic P. infections are difficult to treat, primarily owing antibiotic treatment failure, which is often observed the absence of antimicrobial resistance. In MADs, forms biofilm-like aggregates within luminal mucus. While contribution mucin hyperconcentration towards tolerance has been described, mechanism for driven and influence have not fully elucidated. this study, we investigated flagellar motility aggregate formation as it relates diseased mucus environment. We found that loss resulted increased aggregation multiple classes antibiotics. Further, differential roles motAB motCD stators, power flagellum. Additionally, control fliC expression was important a strain constitutively expressing unable form highly susceptible treatment. Lastly, demonstrate neutrophil elastase, an abundant immune mediator biomarker infection, promotes by impairing motility. Collectively, these results highlight key role deepens our understanding how MADs environment . IMPORTANCE Antibiotic recalcitrance primary driver mortality. Mechanisms drive poorly understood. phenotypes related adaptation Loss drives promoting formation. Regulation appears be step inability turn off flagellin poor susceptibility. These deepen tolerant opens novel avenues targets infections.
Language: Английский
Citations
0
BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)
Published: Nov. 11, 2024
The interactions between bacterial pathogens and host cells are characterized by a multitude of complexities, leading to wide range heterogeneous outcomes. Despite extensive research, we still have limited understanding how motility in complex environments impacts their ability tolerate antibiotics adhere mammalian cell surfaces. challenge lies unraveling the complexity these developing quantitative microscopy approaches predict behavior populations.
Language: Английский
Citations
0
Springer series on biofilms, Journal Year: 2024, Volume and Issue: unknown, P. 259 - 282
Published: Jan. 1, 2024
Language: Английский
Citations
0